Densitometric determination of catecholamine metabolites and 5-hydroxy-indoleacetic acid after two-dimensional thin-layer chromatography on cellulose

1972 ◽  
Vol 37 ◽  
pp. 157-164 ◽  
Author(s):  
K. Breebaart ◽  
A.M.F.H. Haan ◽  
S.K. Wadman
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Indoleacetic Acid ◽  
Two Dimensional ◽  
Catecholamine Metabolites ◽  
Layer Chromatography

scholarly journals The New TLC Method for Separation and Determination of Multicomponent Mixtures of Plant Extracts

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Elżbieta Matysik ◽  
Anna Woźniak ◽  
Roman Paduch ◽  
Robert Rejdak ◽  
Beata Polak ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Gradient System ◽  
Two Dimensional ◽  
Phase Gradient ◽  
One Dimensional ◽  
Dominant Component ◽  
Phase Condition ◽  
Layer Chromatography

The new mode of two-dimensional gradient thin layer chromatography (MGD-2D TLC) has been presented. Short distance development of sample in the first dimension leads to formation of the preconcentrated narrow zones. They are consecutively separated in the second dimension with the mobile phase gradient in several steps of development until the eluent reaches the further end of the chromatographic plate. The use of the above-mentioned technique allows isolating and then identifying the compounds of various polarity from the multicomponent mixture. The practical application of two-dimensional gradient thin layer chromatography has been performed for isolation of the two plant (JuniperusandThymus) oils components as the examples of test mixtures. The experiments have been carried out with the use of silica gel plates as well as a normal phase condition. The results of solute separation with isocratic one-dimensional thin layer chromatography system have been compared with those of two-dimensional gradient system. It has been observed that application of the latter mode leads to almost triplicated number of zones in comparison with the former one. It is purposeful to apply the proposed mode to control the purity of the dominant component or components of the mixture.

scholarly journals Identification of amino acid thiohydantoins directly by thin-layer chromatography and indirectly by gas-liquid chromatography after hydrolysis

1975 ◽  
Vol 147 (3) ◽  
pp. 435-438 ◽  
Author(s):  
M Rangarajan ◽  
A Darbre
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Indirect Method ◽  
Gas Liquid Chromatography ◽  
Two Dimensional ◽  
Layer Chromatography

A method is described for the identification of amino acid thiohydantoins by two-dimensional t.l.c. An indirect method for the determination of amino acid thiohydantoins is described which, after hydrolysis, the corresponding amino acids are determined by g.l.c.

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