A longitudinal study of HCV RNA detected by in situ-RT-PCR in liver biopsy specimens during progression from chronic HCV liver disease to hepatocellular cancer, HCC

Abstract Background.—Histopathologic features of early recurrent hepatitis C after orthotopic liver transplantation (OLTx) may be modified by immunosuppressive therapy or complicated by other conditions. Hepatitis C virus (HCV) RNA level usually increases after OLTx, but its correlation to histologic findings is not clear. Objective.—To evaluate the histologic findings of early recurrent hepatitis C in liver allografts and its correlation to serum HCV RNA level. Methods.—We studied 14 patients who underwent OLTx for chronic HCV infection. Thirty liver biopsy specimens and HCV RNA levels of 22 corresponding plasma samples obtained during the first 6 months following OLTx were analyzed. The control group (9 patients, 25 biopsy specimens) was chosen at random from patients with chronic liver disease other than HCV who were undergoing OLTx, and all tested negative for HCV RNA by polymerase chain reaction after OLTx. Results.—Statistically significant pathological features of early recurrent HCV infection were the number of acidophilic bodies, piecemeal necrosis, lymphocyte predominance in the portal tracts, and fibrous septum. These findings and histologic activity index scores increased with time after OLTx. The HCV RNA levels determined by branched DNA assay showed no significant correlation with histologic features. However, patients with higher histologic activity index scores tended to have higher RNA levels. Conclusions.—Liver biopsy specimens are helpful for the diagnosis or confirmation of early recurrent hepatitis C in liver allografts, but serial biopsy specimens are sometimes required for definite diagnosis. The HCV RNA levels are usually higher in patients who display signs of more severe liver damage.

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Demonstration and Distribution of HCV RNA Sequences by in situ Hybridization and HCV-Related Proteins by Immunohistochemistry in the Liver Tissue of Patients with Chronic HCV Infection

Pathobiology ◽

10.1159/000163956 ◽

1995 ◽

Vol 63 (5) ◽

pp. 239-248 ◽

Cited By ~ 10

Author(s):

Domenico Sansonno ◽

Vito Cornacchiulo ◽

Anna Rina Iacobelli ◽

Pietro Gatti ◽

Maria Di Stasi ◽

...

Keyword(s):

In Situ Hybridization ◽

Liver Tissue ◽

Hcv Infection ◽

Hcv Rna ◽

Rna Sequences ◽

Chronic Hcv Infection ◽

Chronic Hcv ◽

Related Proteins

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Phenotypic and functional characteristics of lymphocytes isolated from liver biopsy specimens from patients with active liver disease

Hepatology ◽

10.1002/hep.1840150512 ◽

1992 ◽

Vol 15 (5) ◽

pp. 816-823 ◽

Cited By ~ 38

Author(s):

Kensaku Hata ◽

David H. van Thiel ◽

Ronald B. Herberman ◽

Theresa L. Whiteside

Keyword(s):

Liver Disease ◽

Liver Biopsy ◽

Functional Characteristics ◽

Biopsy Specimens

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Detection and Localization by In Situ Molecular Biology Techniques and Immunohistochemistry of Hepatitis C Virus in Livers of Chronically Infected Patients

Journal of Histochemistry & Cytochemistry ◽

10.1177/002215549804600510 ◽

1998 ◽

Vol 46 (5) ◽

pp. 653-660 ◽

Cited By ~ 18

Author(s):

Francine M. Walker ◽

Marie-Christine Dazza ◽

Marie-Christine Dauge ◽

Olivier Boucher ◽

Christophe Bedel ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Interferon Α ◽

Rt Pcr ◽

Biopsy Specimens ◽

Bile Ductule ◽

Liver Biopsies ◽

Cytoplasmic Signals ◽

Detection And Localization

Hepatitis C virus (HCV) detection in the livers of chronically infected patients remains a debatable issue. We used immunohistochemistry, in situ hybridization (ISH) alone or after microwave heating with FITC-labeled probes, RT-PCR with unlabeled primers followed by ISH (RT-PCR-ISH), and in situ RT-PCR with FITC-labeled primers (in situ RT-PCRd) to localize the virus in 38 liver biopsy specimens from 21 chronically infected HCV patients treated with interferon-α (IFN-α). Biopsies were taken at the beginning and end of IFN-α treatment and 1 year later. Results were compared with that of HCV-PCR in serum. RT-PCR-ISH and in situ RT-PCRd showed HCV signal in all liver biopsies even in responders with seronegative HCV PCR. This signal was intranuclear, diffuse, or peripheral, in hepatocytes, bile ductule cells, and lymphocytes. Cytoplasmic signals were occasionally observed. Whereas the percentage of labeled hepatocytes remained constant, the number of labeled lymphoid follicles decreased after INF-α therapy. Immunohistochemistry resulted in the same pattern of positivity but it was weaker and inconstant. This study indicates the persistency of HCV latency in IFN-α responders 1 year after IFN-α treatment cessation, a finding that certainly deserves confirmation.

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HCV Seroreactivity and Detection of HCV RNA in Hepatitis Cases

Annals of the National Academy of Medical Sciences (India) ◽

10.1055/s-0040-1712789 ◽

2018 ◽

Vol 54 (02) ◽

pp. 074-077

Author(s):

Mahantesh B Nagamoti ◽

Chidanand S Patil ◽

Jyoti M Nagamoti

Keyword(s):

Polymerase Chain Reaction ◽

Liver Biopsy ◽

Hcv Rna ◽

Western Region ◽

Liver Pathology ◽

Rt Pcr ◽

Chain Reaction ◽

Polymerase Chain ◽

Cellular Carcinoma ◽

Routine Investigations

ABSTRACT Background: Hepatitis C virus (HCV) known to be associated with wide variety of liver pathology. It is less studied in India as compared to western region. Methods: Suspected patients sera screened for HCV by ELISA and conﬁrmed with reverse transcription polymerase chain reaction (RT-PCR) along with routine investigations and liver proﬁle. All HCV positive patients were undergone liver biopsy. Results: All 24 HCV ELISA reactive and two ELISA indeterminate sera are conﬁrmed by RT- PCR. The liver biopsy of these patients showed normal picture (19.2%), Acute hepatitis (11.5%), Chronic hepatitis (23.7%), Cirrhosis (34.72%), Hepato-cellular carcinoma (HCC) (15.38%). ALT levels were not signiﬁcant. Conclusion: All the suspected HCV cases need to be conﬁrmed for HCV by RT-PCR.

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Quantitative measurement of the histological features of alpha-1 antitrypsin deficiency-associated liver disease in biopsy specimens

PLoS ONE ◽

10.1371/journal.pone.0256117 ◽

2021 ◽

Vol 16 (8) ◽

pp. e0256117

Author(s):

George Marek ◽

Amy Collinsworth ◽

Chen Liu ◽

Mark Brantly ◽

Virginia Clark

Keyword(s):

Liver Disease ◽

Liver Biopsy ◽

Periodic Acid ◽

Therapeutic Interventions ◽

Periodic Acid Schiff ◽

Biopsy Specimens ◽

Antitrypsin Deficiency ◽

Alpha 1 Antitrypsin Deficiency ◽

Alpha 1 Antitrypsin ◽

Relationship Of

Background Pathological mutations in Alpha-1 Antitrypsin (AAT) protein cause retention of toxic polymers in the hepatocyte endoplasmic reticulum. The risk for cirrhosis in AAT deficiency is likely directly related to retention of these polymers within the liver. Polymers are classically identified on liver biopsy as inclusion bodies by periodic acid schiff staining after diastase treatment and immunohistochemistry. However, characterization of the polymer burden within a biopsy sample is limited to a semi-quantitative scale as described by a pathologist. Better methods to quantify polymer are needed to advance our understanding of pathogenesis of disease. Therefore, we developed a method to quantify polymer aggregation from standard histologic specimens. In addition, we sought to understand the relationship of polymer burden and other histologic findings to the presence of liver fibrosis. Methods Liver samples from a well-categorized AATD cohort were used to develop histo-morphometric tools to measure protein aggregation. Results Whole-slide morphometry reliably quantifies aggregates in AATD individuals. Despite very low levels of inclusions present (0–0.41%), accumulation of globules is not linear and is associated with higher fibrosis stages. Immunohistochemistry demonstrates that fibrosis is associated with polymer accumulation and not total AAT. A proportion of patients were found to be “heavy accumulators” with a polymer burden above the upper 25% of normal distribution. Males had significantly more liver inclusions and polymer than females. These measurements also highlight interrelated phenotypes of hepatocellular degeneration and autophagy in AATD liver disease. Conclusion Quantitative inclusion analysis measures AAT accumulation in liver biopsy specimens. Quantification of polymer may identify individuals at risk for progressive disease and candidates for therapeutic interventions. Furthermore, these methods may be useful for evaluating efficacy of drugs targeting accumulation of AAT.

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Lymphocyte populations in liver biopsy specimens from patients with chronic liver disease.

Gut ◽

10.1136/gut.18.6.472 ◽

1977 ◽

Vol 18 (6) ◽

pp. 472-475 ◽

Cited By ~ 51

Author(s):

J Sanchez-Tapias ◽

H C Thomas ◽

S Sherlock

Keyword(s):

Liver Disease ◽

Liver Biopsy ◽

Chronic Liver Disease ◽

Chronic Liver ◽

Biopsy Specimens ◽

Lymphocyte Populations

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Interest of the in situ hybridization after reverse transcription polymerase chain reaction (RT-PCR-ISH) for HCV RNA detection in liver of interferon ? treated patients with chronic hepatitis C comparison with PCR in serum . Department of Pathology Bichat-Claude Bernard Hospital, Department of Pathology and Hepatology Beaujon Hospital, INSERM U24, and INSERM U13, Paris, France

Hepatology ◽

10.1016/0270-9139(95)95747-2 ◽

1995 ◽

Vol 22 (4) ◽

pp. A506

Keyword(s):

Polymerase Chain Reaction ◽

Chronic Hepatitis ◽

Hcv Rna ◽

Rt Pcr ◽

Chain Reaction ◽

Rna Detection ◽

Hospital Department ◽

Claude Bernard ◽

Polymerase Chain

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Hepatic histology in hepatitis C virus carriers coinfected with hepatitis G virus

Gut ◽

10.1136/gut.42.1.103 ◽

1998 ◽

Vol 42 (1) ◽

pp. 103-106 ◽

Cited By ~ 17

Author(s):

J Petrik ◽

L Guella ◽

D G D Wight ◽

G M Pearson ◽

J Hinton ◽

...

Keyword(s):

Hepatitis C Virus ◽

Liver Disease ◽

Hepatitis C ◽

Liver Biopsy ◽

Chronic Liver Disease ◽

Chronic Liver ◽

Hcv Rna ◽

Hepatitis G Virus ◽

Histological Features ◽

Hepatitis G

Background—A novel flavivirus has been described recently and designated hepatitis G virus (HGV). The virus is transmitted by the parenteral route but it is uncertain whether it is associated with chronic liver disease because liver biopsy is difficult to justify in this group.Aims—To examine histological features of liver biopsy in patients infected with hepatitis C virus (HCV) according to the presence or absence of HCV and HGV RNA.Methods—One hundred and thirty one consecutive HCV carriers undergoing staging liver biopsy were studied retrospectively. In each, HCV RNA and HGV RNA were detected by reverse transcription polymerase chain reaction on serum samples collected at the time of biopsy. The presence of each RNA was correlated with histological features blind to the RNA results; individual histological features of inflammation or fibrosis were scored separately.Results—Nineteen patients were positive for both HGV and HCV RNA in serum, 91 were positive for HCV RNA alone, two were positive for HGV RNA alone, and 19 were negative for both RNA species. Neither age nor sex differed between the groups; a greater proportion of intravenous drug users were HGV RNA positive, but this was not statistically significant. There was no effect of HGV coinfection on the stage of fibrosis or any other histological parameter except steatosis; patients with HCV and HGV RNA had a higher mean score for fat than those patients with HCV RNA alone (p<0.05).Conclusions—HGV coinfection has no important effects on histological features in chronic HCV carriers. It is unlikely that HGV infection causes chronic liver disease.

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