A growth chamber for the production of 14C-labeled salt marsh plants and its application to smooth cordgrass, Spartina alterniflora Loisel

1974 ◽  
Vol 14 (3) ◽  
pp. 251-259 ◽  
Author(s):  
Randolph L. Ferguson ◽  
Richard B. Williams

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Catherine M. Van Dongen ◽  
Logan M. Rakes ◽  
Joseph W. Moriarty ◽  
Sam Mason ◽  
Matthias Leu




2000 ◽  
Vol 66 (9) ◽  
pp. 3814-3822 ◽  
Author(s):  
Charles R. Lovell ◽  
Yvette M. Piceno ◽  
Joseph M. Quattro ◽  
Christopher E. Bagwell

ABSTRACT N2 fixation by diazotrophic bacteria associated with the roots of the smooth cordgrass, Spartina alterniflora, is an important source of new nitrogen in many salt marsh ecosystems. However, the diversity and phylogenetic affiliations of these rhizosphere diazotrophs are unknown. Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified nifH sequence segments was used in previous studies to examine the stability and dynamics of the Spartina rhizosphere diazotroph assemblages in the North Inlet salt marsh, near Georgetown, S.C. In this study, plugs were taken from gel bands from representative DGGE gels, thenifH amplimers were recovered and cloned, and their sequences were determined. A total of 59 sequences were recovered, and the amino acid sequences predicted from them were aligned with sequences from known and unknown diazotrophs in order to determine the types of organisms present in the Spartina rhizosphere. We recovered numerous sequences from diazotrophs in the γ subdivision of the division Proteobacteria(γ-Proteobacteria) and from various anaerobic diazotrophs. Diazotrophs in the α-Proteobacteria were poorly represented. None of the Spartina rhizosphere DGGE band sequences were identical to any known or previously recovered environmental nifH sequences. TheSpartina rhizosphere diazotroph assemblage is very diverse and apparently consists mainly of unknown organisms.



2011 ◽  
Vol 93 (4) ◽  
pp. 415-419 ◽  
Author(s):  
Pedro N. Carvalho ◽  
Pedro Nuno R. Rodrigues ◽  
Rafael Evangelista ◽  
M. Clara P. Basto ◽  
M. Teresa S.D. Vasconcelos
Keyword(s):  


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