Immobilization of viral antigens on filter paper for a [125I]staphylococcal protein a immunoassay: A rapid and sensitive technique for detection of herpes simplex virus antigens and antiviral antibodies

1979 ◽  
Vol 29 (4) ◽  
pp. 369-386 ◽  
Author(s):  
Patrick H. Cleveland ◽  
Douglas D. Richman ◽  
Michael N. Oxman ◽  
M. Gary Wickham ◽  
Perry S. Binder ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Filter Paper ◽  
Protein A ◽  
Staphylococcal Protein A ◽  
Sensitive Technique ◽  
Staphylococcal Protein ◽  
Viral Antigens ◽  
Virus Antigens ◽  
Simplex Virus

scholarly journals Immunoelectron microscopic localization of herpes simplex virus antigens in infected cells using the unlabeled antibody-enzyme method.

1979 ◽  
Vol 27 (11) ◽  
pp. 1455-1461 ◽  
Author(s):  
B L Hansen ◽  
G N Hansen ◽  
B F Vestergaard
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Reaction Products ◽  
Viral Antigens ◽  
Infected Cells ◽  
Viral Morphogenesis ◽  
Simplex Virus ◽  
Unlabeled Antibody ◽  
Hsv 1

Subcellular localization of viral antigens was demonstrated during viral morphogenesis using herpes simplex virus type 1 (HSV-1) infected monolayers of rabbit cornea cells. The localization was done by immunoelectron microscopy employing the peroxidase-antiperoxidase (PAP) immunocytochemical technique and the postembedding staining method. The localization of viral antigens was followed at time intervals during infection from 2 to 19 hr. After exposure of sections to either polyspecific antibodies against total HSV-1 antigens or monospecific antibodies against HSV-1 antigen No. 8, specific immunological reaction products were identified both in the cytoplasm and nucleus after 2 hr. The distribution and quantity of reaction products varied in the infected cells during the viral morphogenesis. The present results on the subcellular distribution of the HSV-1 antigens are related to current biochemical findings.

HERPES-SIMPLEX-VIRUS ANTIGENS IN NORMAL CERVICAL CELLS

The Lancet ◽  
1976 ◽  
Vol 307 (7959) ◽  
pp. 597 ◽  
Author(s):  
AlexanderS. Pacsa ◽  
Lajos Kummerländer ◽  
Bela Pejtsik ◽  
Maria Simon ◽  
Kalman Pali
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Antigens ◽  
Cervical Cells ◽  
Simplex Virus

SB-restricted presentation of influenza and herpes simplex virus antigens to human T-Iymphocyte clones

Nature ◽  
1983 ◽  
Vol 301 (5902) ◽  
pp. 716-718 ◽  
Author(s):  
David D. Eckels ◽  
Phil Lake ◽  
Jonathan R. Lamb ◽  
Armead H. Johnson ◽  
Stephen Shaw ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Antigens ◽  
Simplex Virus

scholarly journals PREVENTION OF CELL-TO-CELL SPREAD OF HERPES SIMPLEX VIRUS BY LEUKOCYTES

1973 ◽  
Vol 137 (3) ◽  
pp. 706-720 ◽  
Author(s):  
Donald Louis Lodmell ◽  
Akira Niwa ◽  
Kozaburo Hayashi ◽  
Abner Louis Notkins
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Spread ◽  
Viral Antigens ◽  
Antiviral Antibody ◽  
Infected Cells ◽  
Immunological Destruction ◽  
Simplex Virus ◽  
The Relationship ◽  
Cell Spread

Antibody to herpes simplex virus (HSV) plus complement destroyed HSV-infected cells but did not stop the spread of the infection. Studies on the relationship between the time of appearance of viral antigens on the cell surface, immunological destruction of the cells by antiviral antibody and complement, and transfer of the virus to adjacent cells showed that the virus spread from infected to uninfected cells before the infected cells were susceptible to immunological destruction. Incubation of infected monolayers with leukocytes, however, stopped the spread of the virus by nonspecifically damaging both infected and uninfected cells and by presumably breaking intercellular bridges. When leukocytes were removed from infected monolayers, viral plaques developed. If, however, antiviral antibody and complement were added to monolayers before the leukocytes were removed, the development of plaques was prevented. These findings suggest that both antibody and leukocytes are needed to cure HSV infections.

A sensitive enzyme-linked immunosorbent assay for the detection of herpes simplex virus antigens

1987 ◽  
Vol 17 (1-2) ◽  
pp. 159-174 ◽  
Author(s):  
J.M. Middeldorp ◽  
A.M. Hooymans ◽  
A.J.H.F. Kocken ◽  
A.M. van Loon ◽  
J.A. Emsbroek ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Antigens ◽  
Simplex Virus

scholarly journals Efficiency of Reconstitution of Immunoglobulin G from Blood Specimens Dried on Filter Paper and Utility in Herpes Simplex Virus Type-Specific Serology Screening

2002 ◽  
Vol 9 (6) ◽  
pp. 1338-1342 ◽  
Author(s):  
Wayne R. Hogrefe ◽  
Carolyn Ernst ◽  
Xin Su
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Immunoglobulin G ◽  
Herpes Simplex Virus Type ◽  
Filter Paper ◽  
Serum Samples ◽  
Blood Samples ◽  
The Mean ◽  
Simplex Virus ◽  
Hsv 1

ABSTRACT The performance of studies using sera from remote locations is greatly facilitated if whole-blood samples dried on filter paper are shown to be compatible with the serologic assay being employed. Since dried blood samples do not require immediate refrigeration, occupy little space, and are easily transported, they may be used for evaluating the seroprevalence of herpes simplex virus type 1 (HSV-1) and HSV-2 in geographic locations where laboratory resources are limited. We evaluated the utility of dried blood samples for the detection of type-specific HSV antibodies. The efficiency of using immunoglobulin G (IgG) eluted from dried blood samples was found to be consistent with measurement of IgG concentrations in most corresponding serum samples. The ratio of the mean IgG concentration for all dried blood samples to the mean IgG concentration for the corresponding sera was 1:29. When the 1:29 ratio was applied to each of the 22 pairs of samples, there was a deviation of less than 15% between concentrations in the dried blood sample and in the corresponding serum sample in 19 of the pairs. No positive or negative bias was detected for the IgG eluted from dried blood. The presence of HSV-1 and HSV-2 antibodies was determined in the paired dried blood and serum samples, and no differences in the HSV serostatuses were detected for 43 of the 44 pairs. One pair's serostatus varied, with the serum sample being weakly positive for HSV-1 and the dried blood sample results being equivocal. The detection of HSV antibodies was generally consistent for dried blood samples stored frozen for over 1 year or at room temperature for 30 days, although decreased reactivities were found in a few samples.

Sign in / Sign up

Export Citation Format

Share Document