An enzyme-linked immunosorbent assay for antistriational antibodies associated with myasthenia gravis and thymoma: Comparison with indirect immunofluorescence

1983 ◽  
Vol 59 (3) ◽  
pp. 301-314 ◽  
Author(s):  
Jon A. Carrano ◽  
Nigel R. Swanson ◽  
Roger L. Dawkins
Keyword(s):  
Myasthenia Gravis ◽  
Immunosorbent Assay ◽  
Indirect Immunofluorescence ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals Comparative Analysis of BIOCHIP Mosaic-Based Indirect Immunofluorescence with Enzyme-Linked Immunosorbent Assay for Diagnosing Myasthenia Gravis

Diagnostics ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2098
Author(s):  
Caterina Maria Gambino ◽  
Luisa Agnello ◽  
Bruna Lo Sasso ◽  
Concetta Scazzone ◽  
Rosaria Vincenza Giglio ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Myasthenia Gravis ◽  
Immunosorbent Assay ◽  
Indirect Immunofluorescence ◽  
Simultaneous Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cohen’S Kappa ◽  
Cohen's Kappa ◽  
First Time ◽  
Anti Acetylcholine

Background: The detection of anti-acetylcholine receptor (AChR) and anti-muscle-specific tyrosine kinase (MuSK) antibodies is useful in myasthenia gravis (MG) diagnosis and management. BIOCHIP mosaic-based indirect immunofluorescence is a novel analytical method, which employs the simultaneous detection of anti-AChR and anti-MuSK antibodies in a single miniature incubation field. In this study, we compare, for the first time, the BIOCHIP MG mosaic with conventional enzyme-linked immunosorbent assay (ELISA) in the diagnosis of MG. Methods: A total of 71 patients with MG diagnosis were included in the study. Anti-AChR and anti-MuSK antibodies were measured separately by two different ELISA and simultaneously by BIOCHIP. The results were then compared. Results: The overall concordance between ELISA and BIOCHIP for anti-AChR reactivity was 74%. Cohen’s kappa was 0.51 (95% CI 0.32–0.71), which corresponds to 90% of the maximum possible kappa (0.57), given the observed marginal frequencies. The overall concordance for anti-MuSK reactivity was 84%. Cohen’s kappa was 0.11 (95% CI 0.00–0.36), which corresponds to 41% of the maximum possible kappa (0.27). Conclusion: The overall concordance among assays is not optimal.

scholarly journals Paraneoplastic pemphigus: A trait d’union between dermatology and oncology

2015 ◽  
Vol 1 (2) ◽  
Author(s):  
Dario Didona ◽  
Biagio Didona ◽  
Antonio G Richetta ◽  
Carmen Cantisani ◽  
Elisa Moliterni ◽  
...  
Keyword(s):  
Autoimmune Disease ◽  
Clinical Features ◽  
Immunosorbent Assay ◽  
Indirect Immunofluorescence ◽  
Laboratory Tests ◽  
Diagnostic Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Paraneoplastic Pemphigus ◽  
Pathological Evaluation

<p style="margin: 0cm 0cm 8pt; text-align: justify; line-height: 150%;">Paraneoplastic pemphigus is a rare autoimmune disease of the skin associated with neoplasm. Nowadays, the pathogenesis of paraneoplastic pemphigus is not fully understood. Due to its rarity, various criteria have been proposed for the diagnosis. For this reason, several diagnostic methods have been considered useful for the diagnosis of paraneoplastic pemphigus including indirect immunofluorescence, direct immune of fluorescence, immunoprecipitation, immunoblotting, and enzyme-linked immunosorbent assay (ELISA). However, the polymorphic clinical features and the various results of laboratory tests and pathological evaluation present a challenge for the clinicians.</p>

scholarly journals Enzyme-Linked Immunosorbent Assay Screening Then Indirect Immunofluorescence Confirmation of Antinuclear Antibodies

2011 ◽  
Vol 135 (5) ◽  
pp. 678-684 ◽  
Author(s):  
Susan S. Copple ◽  
Allen D. Sawitzke ◽  
Andrew M. Wilson ◽  
Anne E. Tebo ◽  
Harry R. Hill
Keyword(s):  
Immunosorbent Assay ◽  
Indirect Immunofluorescence ◽  
Antinuclear Antibodies ◽  
Enzyme Linked Immunosorbent Assay
Sign in / Sign up

Export Citation Format

Share Document