scholarly journals Paraneoplastic pemphigus: A trait d’union between dermatology and oncology

2015 ◽  
Vol 1 (2) ◽  
Author(s):  
Dario Didona ◽  
Biagio Didona ◽  
Antonio G Richetta ◽  
Carmen Cantisani ◽  
Elisa Moliterni ◽  
...  
Keyword(s):  
Autoimmune Disease ◽  
Clinical Features ◽  
Immunosorbent Assay ◽  
Indirect Immunofluorescence ◽  
Laboratory Tests ◽  
Diagnostic Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Paraneoplastic Pemphigus ◽  
Pathological Evaluation

<p style="margin: 0cm 0cm 8pt; text-align: justify; line-height: 150%;">Paraneoplastic pemphigus is a rare autoimmune disease of the skin associated with neoplasm. Nowadays, the pathogenesis of paraneoplastic pemphigus is not fully understood. Due to its rarity, various criteria have been proposed for the diagnosis. For this reason, several diagnostic methods have been considered useful for the diagnosis of paraneoplastic pemphigus including indirect immunofluorescence, direct immune of fluorescence, immunoprecipitation, immunoblotting, and enzyme-linked immunosorbent assay (ELISA). However, the polymorphic clinical features and the various results of laboratory tests and pathological evaluation present a challenge for the clinicians.</p>

Clinical and Immunologic Characterization in 26 Indian Pemphigus Patients

2013 ◽  
Vol 17 (5) ◽  
pp. 321-331 ◽  
Author(s):  
Daisuke Tsuruta ◽  
Amrinder J. Kanwar ◽  
Keshavamurthy Vinay ◽  
Shunpei Fukuda ◽  
Hiroshi Koga ◽  
...  
Keyword(s):  
Clinical Features ◽  
Immunosorbent Assay ◽  
Indirect Immunofluorescence ◽  
Pemphigus Vulgaris ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pemphigus Foliaceus ◽  
Lower Sensitivity ◽  
Before And After ◽  
Desmoglein 3

Background: Pemphigus shows geographically variable characteristics. Objective: To study the clinical and immunologic characteristics of Indian pemphigus patients before and after treatment. Methods: Twenty-six Indian pemphigus patients were analyzed with regard to age, gender, clinical features, treatments and response, the results of histopathology, direct and indirect immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), and immunoblot analyses. Results: There were 22 pemphigus vulgaris (PV) and 4 pemphigus foliaceus (PF) patients. Direct and indirect IF was positive in 95.8% and 56% of patients, respectively. Indices of ELISA were lower in our study. Immunoblot assays detected the 130 kDa desmoglein-3 in 10 PV patients and the 160 kDa desmoglein-1 in 1 PV patient; 190 kDa periplakin was unexpectedly detected in 8 patients. Conclusion: Indian pemphigus patients showed several unique characteristics, including younger population, predominance of PV, low ELISA indices, lower sensitivity of indirect IF and immunoblotting, and the presence of the 190 kDa periplakin in nearly one-third of patients.

scholarly journals SARS-CoV-2 IgG Antibodies Seroprevalence and Sera Neutralizing Activity in MEXICO: A National Cross-Sectional Study during 2020

2021 ◽  
Vol 9 (4) ◽  
pp. 850
Author(s):  
José Esteban Muñoz-Medina ◽  
Concepción Grajales-Muñiz ◽  
Angel Gustavo Salas-Lais ◽  
Larissa Fernandes-Matano ◽  
Constantino López-Macías ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Neutralizing Antibodies ◽  
Herd Immunity ◽  
Cross Sectional Study ◽  
Molecular Techniques ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Neutralizing Activity

Until recently, the incidence of COVID-19 was primarily estimated using molecular diagnostic methods. However, the number of cases is vastly underreported using these methods. Seroprevalence studies estimate cumulative infection incidences and allow monitoring of transmission dynamics, and the presence of neutralizing antibodies in the population. In February 2020, the Mexican Social Security Institute began conducting anonymous unrelated sampling of residual sera from specimens across the country, excluding patients with fever within the previous two weeks and/or patients with an acute respiratory infection. Sampling was carried out weekly and began 17 days before Mexico’s first officially confirmed case. The 24,273 sera obtained were analyzed by chemiluminescent-linked immunosorbent assay (CLIA) IgG S1/S2 and, later, positive cases using this technique were also analyzed to determine the rate of neutralization using the enzyme-linked immunosorbent assay (ELISA). We identified 40 CLIA IgG positive cases before the first official report of SARS-CoV-2 infection in Mexico. The national seroprevalence was 3.5% in February and 33.5% in December. Neutralizing activity among IgG positives patients during overall study period was 86.1%. The extent of the SARS-CoV-2 infection in Mexico is 21 times higher than that reported by molecular techniques. Although the general population is still far from achieving herd immunity, epidemiological indicators should be re-estimated based on serological studies of this type.

Outlooks of synthetic mycobacterial antigens in serological diagnostics of leprosy

2020 ◽  
Vol 18 (4) ◽  
pp. 164-168
Author(s):  
A.G. Korolyova-Ushakova ◽  
◽  
E.V. Baranova ◽  
S.G. Ignatov ◽  
P.V. Solov’ev ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Biopsy Specimen ◽  
Histological Study ◽  
Mycobacterium Leprae ◽  
Diagnostic Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
Standard Methods ◽  
Skin Biopsy Specimen ◽  
Mycobacterial Antigens ◽  
Serological Diagnostics

Despite the fact that the incidence of leprosy in Russia is sporadic, the number of newly identified patients has increased in recent years. In 2017–2018, 4 new cases of leprosy were registered in Russia. The standard methods of research for diagnosis, in addition to the clinical picture, are bacterioscopic study of the skin scarificates and histological study of the skin biopsy specimen. Currently, additional methods are being developed and used to confirm the diagnosis of leprosy, namely, modern serological and genetic diagnostic methods. To use methods such as enzyme-linked immunosorbent assay (ELISA) and membrane immunochromatographic analysis (leprosy LF serotest), it is appropriate to use domestic synthetic mycobacterial antigens (SMA). Key words: Mycobacterium leprae, leprosy, synthetic mycobacterial antigens, PGL-1(phenolic glycolipid-1), LAM (lipoarabinomannan), serodiagnostics, enzyme-linked immunosorbent assay (ELISA), lateral flow (LF) test, BSA (bovine serum albumin)

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