A flow cytometric method for measuring lymphocyte proliferation directly from tissue culture plates using Ki-67 and propidium iodide

1987 ◽  
Vol 105 (1) ◽  
pp. 97-105 ◽  
Author(s):  
M. Palutke ◽  
D. KuKuruga ◽  
P. Tabaczka
Keyword(s):  
Tissue Culture ◽  
Propidium Iodide ◽  
Lymphocyte Proliferation ◽  
Ki 67 ◽  
Flow Cytometric Method ◽  
Flow Cytometric

A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells

Cytometry ◽  
1994 ◽  
Vol 17 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Francis Belloc ◽  
Patrice Dumain ◽  
Michel R. Boisseau ◽  
Claudine Jalloustre ◽  
Josy Reiffers ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Propidium Iodide ◽  
Cell Cycle Analysis ◽  
Hoechst 33342 ◽  
Flow Cytometric Method ◽  
Flow Cytometric

scholarly journals Optimization of the method for isolation of epithelial cells from the non-glandular part of the rat stomach for flow cytometry

2020 ◽  
Vol 90 (5) ◽  
pp. 517-525
Author(s):  
Gordana Joksić ◽  
◽  
Jelena Filipović Tričković ◽  
Mileva Mićić ◽  
Ivana Joksić ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Epithelial Cells ◽  
Propidium Iodide ◽  
Cellular Proliferation ◽  
Immunohistochemical Detection ◽  
Proliferating Cells ◽  
Ki 67 ◽  
Tert Butyl ◽  
Flow Cytometric ◽  
Propidium Iodide Staining

Traditional methods in cell proliferation studies are based on immunohistochemical detection of proliferating cells in the target tissue. Since they are time consuming, optimization of novel, more efficient methods is important for large scale proliferation studies. In this study, we aimed to optimize the isolation of single epithelial rat forestomach cells for flow cytometry. As a marker of cellular proliferation we used the Ki-67 antibody to detect this nuclear protein expressed in proliferating cells. We also performed immunohistochemical detection of Ki-67 positive cells and propidium iodide staining to validate the results. 3-tert- butyl -4-hydroxyanisole was used as the positive control to ensure cellular proliferation. The results showed that isolation of epithelial cells with collagenase, trypsin and cell strainer ensures great cell viability (>95%) and the purity of the samples. Flow cytometry and immunostaining with the Ki-67 antibody indicated that 3-tert- butyl-4-hydroxyanisole treatment leads to a significant increase in proliferation. A significant positive correlation was observed between the results obtained by immunohistochemistry and flow cytometry, but the flow cytometric data had a smaller measurement error, suggesting the equal sensitivity and greater accuracy of this method. Propidium iodide staining showed that the percentage of cells in the G2+S phase of the cell cycle correlated positively with the percentage of Ki-67 positive cells assessed by flow cytometry, indicating that Ki-67 positive cells reflect an active dividing cell pool. We conclude that the isolation of forestomach epithelial cells described is a simple and reliable method for obtaining viable cells for use in flow cytometry. Compared to immunohistochemistry, flow cytometric detection of the Ki-67 antigen is equally sensitive, but much faster and provides more accurate results.

A sensitive flow cytometric method for measuring the oxidative burst

1997 ◽  
Vol 202 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Michael A Model ◽  
Mark A KuKuruga ◽  
Robert F Todd
Keyword(s):  
Oxidative Burst ◽  
Flow Cytometric Method ◽  
Flow Cytometric

scholarly journals Flow cytometric evaluation of DNA stainability with propidium iodide after histone H1 extraction

Cytometry ◽  
1989 ◽  
Vol 10 (6) ◽  
pp. 726-730 ◽  
Author(s):  
Maria Chiara Giangarè ◽  
Ennio Prosperi ◽  
Guido Pedrali-Noy ◽  
Giovanni Bottiroli
Keyword(s):  
Propidium Iodide ◽  
Histone H1 ◽  
Flow Cytometric ◽  
Flow Cytometric Evaluation

scholarly journals Flow cytometric method for the routine follow‐up of red cell populations after bone marrow transplantation

1997 ◽  
Vol 97 (1) ◽  
pp. 141-145 ◽  
Author(s):  
E. C. M. Hendriks ◽  
A. J. M. De Man ◽  
Y. C. M. Van Berkel ◽  
S. Stienstra ◽  
T. De Witte
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Marrow Transplantation ◽  
Cell Populations ◽  
Red Cell ◽  
Flow Cytometric Method ◽  
Flow Cytometric

scholarly journals Aberrant Ki-67 expression in normal bone marrow revealed by multiparameter flow cytometric analysis

Cytometry ◽  
1991 ◽  
Vol 12 (1) ◽  
pp. 50-63 ◽  
Author(s):  
Dirk R. Van Bockstaele ◽  
Jar Lan ◽  
Hans-W. Snoeck ◽  
Marcel L. Korthout ◽  
Robrecht F. De Bock ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometric Analysis ◽  
Normal Bone Marrow ◽  
Ki 67 ◽  
Normal Bone ◽  
Flow Cytometric

A novel EGFP-CEM-NKr flow cytometric method for measuring antibody dependent cell mediated-cytotoxicity (ADCC) activity in HIV-1 infected individuals

2006 ◽  
Vol 315 (1-2) ◽  
pp. 1-10 ◽  
Author(s):  
Wannee Kantakamalakul ◽  
Kovit Pattanapanyasat ◽  
Surat Jongrakthaitae ◽  
Vatcharain Assawadarachai ◽  
Silawun Ampol ◽  
...  
Keyword(s):  
Adcc Activity ◽  
Flow Cytometric Method ◽  
Flow Cytometric ◽  
Dependent Cell ◽  
Hiv 1

scholarly journals A 5-color flow cytometric method for extended 8-part leukocyte differential

2017 ◽  
Vol 92 (6) ◽  
pp. 498-507 ◽  
Author(s):  
Julien Guy ◽  
Orianne Wagner-Ballon ◽  
Olivier Pages ◽  
François Bailly ◽  
Jessica Borgeot ◽  
...  
Keyword(s):  
Color Flow ◽  
Flow Cytometric Method ◽  
Flow Cytometric

scholarly journals Chromosome doubling in Cattleya tigrina A. Rich

2019 ◽  
Vol 15 (11) ◽  
Author(s):  
Thays Saynara Alves Menezes-Sá ◽  
Maria de Fátima Arrigoni-Blank ◽  
Andréa Santos da Costa ◽  
Janay De Almeida Santos-Serejo ◽  
Arie Fitzgerald Blank ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Propidium Iodide ◽  
Chromosome Doubling ◽  
Flow Cytometric Analysis ◽  
Leaf Tissue ◽  
Stomatal Index ◽  
Chromosome Duplication ◽  
Flow Cytometric ◽  
Young Leaves ◽  
Commercial Value

Chromosome doubling induction in orchids may benefit their production for resulting in flowers of higher commercial value, larger size and higher content of substances that intensify the color and fragrance when compared with diploid orchids. This work aimed to induce and confirm artificial polyploidization, using flow cytometry and stomatal analysis. Explants were treated with colchicine at concentrations of 0, 2.5, 7.5, and 12.5 mM, for 24 and 48 hours and with oryzalin, at concentrations of 0, 10, 30, and 50 μM, for three and six days. For the flow cytometric analysis, a sample of leaf tissue was removed from each plant, crushed to release the nuclei and stained with propidium iodide. In addition to flow cytometry, the ploidy of the antimitotic treated plants was evaluated by stomata analysis. Young leaves were used where the density, functionality and stomatal index were evaluated. Colchicine provided induction of satisfactory polyploidy in C. tigrina at all concentrations and times of exposure, obtaining a greater number of polyploid individuals in the concentration of 12.5 mM for 48 hours. Oryzalin did not induce chromosome duplication at the tested concentrations.

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