An evaluation of the role of cyclic AMP as an intracellular second messenger in Malpighian tubules of the house cricket, Acheta domesticus

1991 ◽  
Vol 37 (8) ◽  
pp. 563-573 ◽  
Author(s):  
G.M. Coast ◽  
O. Cusinato ◽  
I. Kay ◽  
G.J. Goldsworthy
Keyword(s):  
Cyclic Amp ◽  
Second Messenger ◽  
Acheta Domesticus ◽  
Malpighian Tubules ◽  
House Cricket

scholarly journals THE EFFECTS OF ACHETA DIURETIC PEPTIDE ON ISOLATED MALPIGHIAN TUBULES FROM THE HOUSE CRICKET ACHETA DOMESTICUS

1994 ◽  
Vol 187 (1) ◽  
pp. 225-243 ◽  
Author(s):  
G M Coast ◽  
I Kay
Keyword(s):  
Cyclic Amp ◽  
Unit Length ◽  
Phosphodiesterase Inhibitor ◽  
Fluid Secretion ◽  
Acheta Domesticus ◽  
Malpighian Tubules ◽  
Membrane Receptors ◽  
Dependent Manner ◽  
House Cricket ◽  
Dose Dependent

Acheta diuretic peptide (Acheta-DP) is a corticotropin-releasing factor (CRF)-related peptide found in head extracts of the house cricket Acheta domesticus. The peptide causes a dose-dependent increase in fluid secretion by cricket Malpighian tubules isolated in vitro, and the apparent EC50 is 1.3 nmol l-1, which is within the physiological range for a peptide hormone. The CRF antagonist alpha-helical CRF(9-41) blocks the action of Acheta-DP in a dose-dependent manner, and the IC50 is estimated to be in the micromolar range. Addition of Acheta-DP to isolated Malpighian tubules is followed by a rapid and marked increase in the level of intracellular cyclic AMP. This precedes any change in voltage or fluid secretion, which strongly suggests that cyclic AMP is the intracellular mediator of Acheta-DP activity. Consistent with this, diuretic activity is potentiated by the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, and there is a close relationship between the dose­response curves for cyclic AMP production and for fluid secretion. However, exogenous 8-bromo-cyclic AMP does not mimic all the effects of Acheta-DP, and the peptide may have a dual action on isolated tubules. Fluid secretion by tubules dosed repeatedly with Acheta-DP returns to near basal levels after 3­5 h. This cannot be explained by degradation of the peptide, but might be due in part to oxygen and/or metabolite deficiency. However, tubules that are refractory to Acheta-DP can be stimulated by forskolin, 8-bromo-cyclic AMP and extracts of corpora cardiaca, which is indicative of a homologous desensitization of membrane receptors for the diuretic peptide. Differences in the rate of secretion by morphologically distinct regions of cricket Malpighian tubules have been assessed. In unstimulated tubules, the rate of secretion per unit length by the short distal segment is about twice that of the main tubule. However, diuretic peptides (Acheta-DP and achetakinin-I) have little effect on distal tubule secretion, but evoke a two- to fourfold increase in fluid secretion by the main tubule segment.

Serotonin triggers cAMP and PKA-mediated intracellular calcium waves in Malpighian tubules of Rhodnius prolixus

2014 ◽  
Vol 307 (7) ◽  
pp. R828-R836 ◽  
Author(s):  
Paula Gioino ◽  
Brendan G. Murray ◽  
Juan P. Ianowski
Keyword(s):  
Second Messenger ◽  
Fluid Secretion ◽  
Rhodnius Prolixus ◽  
Malpighian Tubules ◽  
Insect Vector ◽  
Excess Water ◽  
Intracellular Ca ◽  
Single Meal ◽  
Hematophagous Insect

Rhodnius prolixus is a hematophagous insect vector of Chagas disease capable of ingesting up to 10 times its unfed body weight in blood in a single meal. The excess water and ions ingested with the meal are expelled through a rapid postprandial diuresis driven by the Malpighian tubules. Diuresis is triggered by at least two diuretic hormones, a CRF-related peptide and serotonin, which were traditionally believed to trigger cAMP as an intracellular second messenger. Recently, calcium has been suggested to act as a second messenger in serotonin-stimulated Malpighian tubules. Thus, we tested the role of calcium in serotonin-stimulated Malpighian tubules from R. prolixus. Our results show that serotonin triggers cAMP-mediated intracellular Ca2+ waves that were blocked by incubation in Ca2+-free saline containing the cell membrane-permeant Ca2+ chelator BAPTA-AM, or the PKA blocker H-89. Treatment with 8-Br-cAMP triggered Ca2+ waves that were blocked by H-89 and BAPTA-AM. Analysis of the secreted fluid in BAPTA-AM-treated tubules showed a 75% reduction in fluid secretion rate with increased K+ concentration, reduced Na+ concentration. Taken together, the results indicate that serotonin triggers cAMP and PKA-mediated Ca2+ waves that are required for maximal ion transport rate.

Membrane Dynamics in the Malpighian Tubules of the House Cricket, Acheta Domesticus

2002 ◽  
Vol 185 (1) ◽  
pp. 43-56 ◽  
Author(s):  
S.R. Hazelton ◽  
V.R. Townsend ◽  
B.E. Felgenhauer ◽  
J.H. Spring
Keyword(s):  
Membrane Dynamics ◽  
Acheta Domesticus ◽  
Malpighian Tubules ◽  
House Cricket

THE ROLE OF LIPID IN THE NUTRITION OF THE HOUSE CRICKET, ACHETA DOMESTICUS L. (ORTHOPTERA: GRYLLIDAE)

1965 ◽  
Vol 43 (1) ◽  
pp. 87-98 ◽  
Author(s):  
J. E. S. Meikle ◽  
J. E. McFarlane
Keyword(s):  
Acheta Domesticus ◽  
Active Principle ◽  
Wheat Germ Oil ◽  
Drastic Reduction ◽  
House Cricket ◽  
Total Absence ◽  
Preoviposition Period ◽  
Absolute Effect ◽  
Reproductive Effect

Experiments were carried out to determine the active principle(s) in wheat-germ oil responsible for the reproductive effect exerted by this substance on the house cricket, Acheta domesticus L., and also to investigate the physiological effects of the substance(s). The active principle is vitamin E. It exerts an absolute effect on the last nymphal stadium of the male, but the female appears to be unaffected. Spermatogenic activity is disrupted in the absence of the vitamin, resulting in drastic reduction or a total absence of sperm cells in the testes. Withholding the vitamin from insects causes the preoviposition period to be lengthened and fewer eggs to be laid.

Excretion in the house cricket (Acheta domesticus): Fine structure of the Malpighian tubules

1988 ◽  
Vol 20 (3) ◽  
pp. 443-460 ◽  
Author(s):  
Shelia R. Hazelton ◽  
Stephen W. Parker ◽  
Jeffrey H. Spring
Keyword(s):  
Fine Structure ◽  
Acheta Domesticus ◽  
Malpighian Tubules ◽  
House Cricket

scholarly journals Effect of Manduca Sexta Diuretic Hormone and related Peptides on isolated Malpigfflan Tubules of the House Cricket Acheta Domesticus (L.)

1992 ◽  
Vol 162 (1) ◽  
pp. 331-338
Author(s):  
GEOFFREY M. COAST ◽  
TIMOTHY K. HAYES ◽  
IAIN KAY ◽  
JUM-SOOK CHUNG
Keyword(s):  
Cyclic Amp ◽  
Manduca Sexta ◽  
Pieris Rapae ◽  
Fluid Secretion ◽  
Acheta Domesticus ◽  
Malpighian Tubules ◽  
In Vivo Studies ◽  
Methionine Residues

Previously, a corticotropin releasing factor (CRF)-like diuretic peptide (Manduca-DH) has been isolated from Manduca sexta and shown to stimulate fluid excretion in vivo in post-eclosion Pieris rapae adults and in pre-wandering postfeeding Manduca sexta larvae. However, Manduca- DH was reported to have no effect on Malpighian tubules in vitro. Manduca-DH and [Nle2,11]-Manduca-DH were synthesized in Texas and assayed in London on isolated Malpighian tubules of Acheta domesticus. Manduca- DH stimulated fluid secretion by about 60% of the maximum response achievable with extracts of corpora cardiaca and increased the production of cyclic AMP. In combination with 10−4 mol l−1 3-isobutyl-l-methyl xanthine (IBMX), Manduca-DH stimulated maximal secretion. A number of CRF-related peptides also stimulated fluid secretion and cyclic AMP production in cricket tubules, and the CRF antagonist α-helical-CRF[9-14] blocked the stimulation of fluid secretion by Manduca-DH. [Nle2,11]-Manduca-DH was more active than Manduca-DH in both assays, suggesting that methionine residues in the natural peptide may become oxidized. Taken in conjunction with previous in vivo studies, the present findings suggest that a Manduca-DH-Mke diuretic peptide is the hormone controlling post-eclosion diuresis in butterflies, and Manduca-DH was shown to stimulate both fluid secretion and cyclic AMP production in Malpighian tubules from 1–12 h posteclosion Pieris rapae adults. The function of the peptide in Manduca sexta is discussed.

THE FORMATION OF THE CUTICLE IN THE HOUSE CRICKET, ACHETA DOMESTICUS (L.), AND THE ROLE OF OENOCYTES

1967 ◽  
Vol 45 (2) ◽  
pp. 181-190 ◽  
Author(s):  
Bernard J. R. Philogène ◽  
J. E. McFarlane
Keyword(s):  
Acheta Domesticus ◽  
Chitin Synthesis ◽  
House Cricket ◽  
Phenolic Metabolism

The cuticle of the house cricket, Acheta domesticus (L.), is made up of three layers: an epicuticle, an exocuticle, and an endocuticle. The epicuticle is laid down first and the endocuticle only after ecdysis. The possible functions of oenocytes in the formation of the cuticle concern the secretion of substances necessary for the wax layer of the epicuticle, a contribution to polysaccharide material involved in chitin synthesis and a role in the phenolic metabolism before sclerotization.

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