Distribution of cutworms in vegetable crops, their harm and the effectiveness of insecticides tested against them

In this article, from the tunnels that infect the underground part of vegetable crops: turnip moth (Agrotis segetum Den.et Schiff), cut worm (A. exclamationis. L.), turn black-C (Xestia c-nigrum L.), wild turn (Euxoa agricola V.), gamma turn (Mamestra suasa Schiff.), tobacco turn (Agrotis obesa B.) and epsolon turn (A. ipsilon Rotl) encounter was detected. Underground cutworms damage to tomatoes is estimated at 6-10% in Navoi, Bukhara and Khorezm regions, 12-20% in the Republic of Karakalpakstan and Surkhandarya, Jizzakh and Syrdarya regions, and 20% in other regions such as Samarkand, Kashkadarya, Andijan, Fergana and Namangan. Up to 31% of underground tunnels were found to be damaged. The damage of the aboveground part cutworms was determined by 18% in Navoi, 15% in Jizzakh, 15-18% in Bukhara and Khorezm regions, 12-15% in Sirdarya, Surkhondarya, Karakalpakstan, 25-30% in the remaining regions. Against worms of months in tomatoes, Deltasis, 2,5% concentration of emulsion (deltametrin), 50% concentration of emulsion in Kurarfon (Profenofos) and Torpedo Jet, 14% soluble powder (Indoxicarb+emamectin benzoate), 2,5% concentration of emulsion in Defentox (deltametrin), when their preparations were tested, 87-93% efficiency was achieved. Contraindications: the drug Kapito 9,3% suspension concentrate (Chlorantraniliprol) is used on the account of 0,45 l/ha to 89,5%, the drug Emafos 42% soluble powder (emamectin benzoate +chlorpyrifos) to 0,6 l/ha. When used on account of 91, 3%, Koragen, 200 soluble powder (chlorantraniliprol) preparation, when applied to 0,2 l/H, 91,6% efficiency was achieved

Effective Use of Emamectin Benzoate for the Management of Spodoptera frugiperda (J. E. Smith) in Maize

Emamectin benzoate has been largely used for controling lepidopterans larvae in grains and fiber crops and, therefore, selection of resistant populations to this pesticide is a real risk if ignored the insect resistance management (IRM) strategies. We studied the susceptibility of Spodoptera frugiperda (Smith, 1797) populations in the state of Mato Grosso - Brazil to Emamectin benzoate as well as a field study about Mode of Action rotation including this compound. Low variations in susceptibility (resistance ratios, 1,01-7,31 fold) were detected among the evalueted S. frugiperda populations. The field study reinforced our monitorings outcome that Emamectin Benzoate controls S. frugiperda and its use in IRM in Mato Grosso is practicable. Susceptibility monitoring should be continuous in order to detect possible increases of tolerance rates not only for this pesticide but also for all compounds used for this pest management aiming to apply early mitigation actions.

Sub-lethal doses of Nucleopolyhedrosis Virus and synthetic ınsecticides alter the biological parameters of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

Resistance management is very important for devising control strategies of polyphagous insect-pests like Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). Considering the importance of resistance management, demographic features of selected and unselected populations of H. armigera were studied in 6 different treatments viz. emamectin benzoate, Helicoverpa armigera Nucleopolyhedrosis Virus (HaNPV), emamectin benzoate+HaNPV, spinetoram, spinetoram+HaNPV and control. Higher values for fecundity, intrinsic rate, the finite rate of increase (λ) were recorded in the control of selected as compared to the rest of treatment. Similarly, higher values for these population parameters viz. oviposition days, fecundity, intrinsic rate, the finite rate of increase were calculated in the unselected control. Similarly, net reproductive rate (R0) for selected and unselected control was higher as compared to the rest of the treatments. It may happen because these kinds of selection pressures can result in decreased fitness of the test insect thus decreased fitness of H. armigera in different treatments was observed as compared to the control. Additionally, quicker development of susceptible insects was observed because susceptible insects were growing without any stressor (xenobiotics) as compared to the rest which contributed to their faster development.

Compatibility of entomopathogenic nematodes with insecticides against the cabbage white butterfly, Pieris rapae L. (Lepidoptera: Pieridae)

Background Compatibility of entomopathogenic nematodes (EPNs) with insecticides is a crucial mainstay of integrated pest management (IPM) programs. This study was designed to evaluate the joint action of EPN species and insecticides when employed to deter 3rd and 4th larval instars of cabbage white butterfly, Pieris rapae L. (Lepidoptera: Pieridae) under laboratory conditions. EPNs [Steinernema carpocapsae (All strain), S. feltiae (Filipjev), Heterorhabditis bacteriophora (HP88), and H. bacteriophora (Ar-4)], at concentrations of 50, 100, and 125 IJs/larva, were tested with 3 insecticides (lambda-cyhalothrin, emamectin benzoate, and indoxacarb) at LC25 and LC50 values. Additionally, expression profiles of 2 detoxification genes (CYP6AE120 and PrGSTs1) when the 4th instar larvae were treated by H. bacteriophora (HP88) and lambda-cyhalothrin were examined. Results Data indicated that statistically significant mortality of 2 larval instars of P. rapae was observed in vitro among EPN species and pesticide concentrations. At concentration of 50 IJs/larva, LT50 values were 2.385 and 3.92 days for S. carpocapsae (All strain) and H. bacteriophora(Ar-4), respectively, on 3rd instar larvae; also, these values were 3.506 and 3.107 days for S. feltiae and H. bacteriophora (Ar-4), respectively, on 4th instar larvae Lambda-cyhalothrin was the most toxic insecticide, followed by emamectin benzoate and indoxacarb at LC25 and LC50, respectively. An additive effect was observed between EPN species with LC25 and LC50 of the tested insecticides, except for lambda-cyhalothrin at LC50 with H. bacteriophora (Ar-4), and indoxacarb, with all EPNs showing antagonistic effects on mortality of 3rd instar larvae after 3 days post-treatment. The interaction between the tested pesticides at LC25 and LC50 and EPN species, showed an additive effect, excluding lambda-cyhalothrin at LC25 with S. carpocapsae (All strain) and LC25 of indoxacarb with H. bacteriophora (Ar-4), which showed potentiation effects. The interaction of S. feltiae (Filipjev) with tested insecticides at LC50 exhibited an antagonistic effect on the mortality of 4th instar P. rapae larvae after 3 days post-treatment. The expression of both CYP6AE120 and PrGSTs1 was significantly up-regulated with lambda-cyhalothrin, followed by H. bacteriophora (HP88) compared to control. Conclusions The findings suggested that combining EPNs and the pesticide concentrations can be a practical strategy for managing P. rapae and could pave the way to using new control technologies in protecting organic farm vegetables from lepidopteran pests.

An Accidental Emamectin Benzoate Poisoning In Child: A Case Report

Background: Emamectin Benzoate has high GABA (Gamma Amino Butyric Acid) receptor affinity and increase chloride membrane permeability. It is the 4'-deoxy-4'-epi-methyl-amino benzoate salt of avermectin B1 (abamectin), obtained from natural fermentation products of Streptomyces Avermitilis.Report: This case report describes the accidental poisoning of Emamectin Benzoate 5% w/ws in a female child. The child was brought to the emergency department(ED) with complaints of nausea, vomiting and abdominal pain. She consumed a packet of “LURA” (5% w/w Soluble Granule (SG) Emamectin Benzoate) supposing it as a packet of “JALJEERA” (a commonly used beverage) since there was no proper labelling. The patient was hemodynamically stable and underwent vigorous gastric lavage with normal saline, activated charcoal and coconut oil. Her blood report was normal for serum electrolytes and renal function. She was admitted in medical ward for symptomatic management and observation. She was given antiemetics, IV fluids and antacids and discharged in 2 days. In follow up after 1 week, she did not have any complain, her gastrointestinal symptoms had completely resolved and she was doing very well.Conclusion: In absence of specific antidote, vigorous gastric lavage with both activated charcoal and coconut oil improves the outcome in Emamectin Benzoate poisoning.

Field testing of synthetic inseticides on fall armyworm (Spodoptera frugiferda J.E. Smith) in corn plant

Fall armyworm is a new pest in Indonesia that is currently of concern because of its widespread attack on corn production areas. FAW infestation in young plants will cause damage up to 90% and even corn plants can die. The study on the control of the fall armyworm (FAW) caused by Spodoptera frugiperda was conducted at the Bajeng Technology Research and Assessment Installation, Gowa, which took place from March-August 2020. The treatments were arranged in a randomized block design with 6 treatments repeated 4 times. The treatments consist of 4 synthetic insecticide treatments derived from 4 active ingredients (emamectin benzoate, spinoteram, carbofuran, chlorantraniliprole + tiamethoxam), one vegetable pesticide (nano pesticide; a.i. citronella, geraniol and citronellol) and one control treatment without pesticides. The treatment application was carried out 2 weeks after planting (WAP) then repeated every 2 weeks until the plants were 8 WAP. The treatment was carried out 2 times in the fegetative phase, 2 times in the generative phase. The plot area of each treatment is 5 x 5 m, the spacing is 70 x 20 cm. The results showed that the most effective insecticide to control FAW was Spinetoram followed by Emamectin Benzoate and Chlorantraniliprole. The average percentage of attacks in the spinetoram treatment was: 24.2% at 4 WAP, then 24.9% at 6 WAP, 29.1% and 37.1% at 10 WAP. The highest average yield was obtained in the insecticide treatment Spinetoram (10.7 t/ha) followed by Emamectin Benzoate (9.3 t/ha) and Chlorantraniliprole (8.9 t/ha), while the lowest yield was obtained in the control treatment (5.2 t/ha).