A functional analysis of the rel gene in Escherichia coli

1969 ◽  
Vol 45 (2) ◽  
pp. 195-203 ◽  
Author(s):  
Niels Fiil
Keyword(s):  
Escherichia Coli ◽  
Functional Analysis

scholarly journals Visualization and functional analysis of the oligomeric states of Escherichia coli heat shock protein 70 (Hsp70/DnaK)

2011 ◽  
Vol 17 (3) ◽  
pp. 313-327 ◽  
Author(s):  
Andrea D. Thompson ◽  
Steffen M. Bernard ◽  
Georgios Skiniotis ◽  
Jason E. Gestwicki
Keyword(s):  
Escherichia Coli ◽  
Functional Analysis ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70

scholarly journals Comprehensive Functional Analysis of Escherichia coli Ribosomal RNA Methyltransferases

2020 ◽  
Vol 11 ◽  
Author(s):  
Philipp Pletnev ◽  
Ekaterina Guseva ◽  
Anna Zanina ◽  
Sergey Evfratov ◽  
Margarita Dzama ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Functional Analysis ◽  
Ribosomal Rna

Functional analysis of the lysis genes of Staphylococcus aureus phage P68 in Escherichia coli

Microbiology ◽  
2005 ◽  
Vol 151 (7) ◽  
pp. 2331-2342 ◽  
Author(s):  
Marian Takáč ◽  
Angela Witte ◽  
Udo Bläsi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Functional Analysis ◽  
Transmembrane Domains ◽  
Class I ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Reading Frame ◽  
Double Stranded Dna ◽  
A Cell

Double-stranded DNA phages of both Gram-positive and Gram-negative bacteria typically use a holin–endolysin system to achieve lysis of their host. In this study, the lysis genes of Staphylococcus aureus phage P68 were characterized. P68 gene lys16 was shown to encode a cell-wall-degrading enzyme, which causes cell lysis when externally added to clinical isolates of S. aureus. Another gene, hol15, was identified embedded in the −1 reading frame at the 3′ end of lys16. The deduced Hol15 protein has three putative transmembrane domains, and thus resembles class I holins. An additional candidate holin gene, hol12, was found downstream of the endolysin gene lys16 based on two predicted transmembrane domains of the encoded protein, which is a typical trait of class II holins. The synthesis of either Hol12 or Hol15 resulted in growth retardation of Escherichia coli, and both hol15 and hol12 were able to complement a phage λ Sam mutation. The hol15 gene has a dual start motif beginning with the codons Met1-Lys2-Met3…. Evidence is presented that the hol15 gene encodes a lysis inhibitor (anti-holin) and a lysis effector (actual holin). As depolarization of the membrane converted the anti-holin to a functional holin, these studies suggested that hol15 functions as a typical dual start motif class I holin. The unusual arrangement of the P68 lysis genes is discussed.

Structure of the chromosomal insertion site for pSAM2: functional analysis in Escherichia coli

1998 ◽  
Vol 28 (2) ◽  
pp. 333-342 ◽  
Author(s):  
Alain Raynal ◽  
Karine Tuphile ◽  
Claude Gerbaud ◽  
Tatjana Luther ◽  
Michel Guerineau ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Functional Analysis ◽  
Insertion Site
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