Increased hypersensitivity induced in tobacco by systemic infection by potato virus Y

Virology ◽  
1968 ◽  
Vol 34 (3) ◽  
pp. 509-520 ◽  
Author(s):  
R.E. Davis ◽  
A.Frank Ross
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Systemic Infection

scholarly journals Potato virus Y (PVY) Isolates from Physalis peruviana are Unable to Systemically Infect Potato or Pepper and Form a Distinct New Lineage Within the PVYC Strain Group

2017 ◽  
Vol 107 (11) ◽  
pp. 1433-1439 ◽  
Author(s):  
Kelsie J. Green ◽  
Mohamad Chikh-Ali ◽  
Randall T. Hamasaki ◽  
Michael J. Melzer ◽  
Alexander V. Karasev
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Polyclonal Antibodies ◽  
Systemic Infection ◽  
Fruit Production ◽  
Genome Sequences ◽  
Physalis Peruviana ◽  
Geographical Separation ◽  
Leaf Deformation ◽  
Cape Gooseberry

Poha, or cape gooseberry (Physalis peruviana L.), is a plant species cultivated in Hawaii for fresh fruit production. In 2015, an outbreak of virus symptoms occurred on poha farms in the South Kohala District of the island of Hawaii. The plants displayed mosaic, stunting, and leaf deformation, and produced poor fruit. Initial testing found the problem associated with Potato virus Y (PVY) infection. Six individual PVY isolates, named Poha1 to Poha6, were collected from field-grown poha plants and subjected to biological and molecular characterization. All six isolates induced mosaic and vein clearing in tobacco, and three of them exhibited O-serotype while the other three reacted only with polyclonal antibodies and had no identifiable serotype. Until now, PVY isolates have been broadly divided into pepper or potato adapted; however, these six PVY isolates from poha were unable to establish systemic infection in pepper and in four tested potato cultivars. Whole-genome sequences for the six isolates were determined, and no evidence of recombination was found in any of them. Phylogenetic analysis placed poha PVY isolates in a distinct, monophyletic “Poha” clade within the PVYC lineage, suggesting that they represented a novel, biologically and evolutionarily unique group. The genetic diversity within this poha PVYC clade was unusually high, suggesting a long association of PVYC with this solanaceous host or a prolonged geographical separation of PVYC in poha in Hawaii.

scholarly journals Resistance levels to two strains of Potato virus Y (PVY) in transgenic potatoes cv. Achat

2001 ◽  
Vol 19 (3) ◽  
pp. 348-350 ◽  
Author(s):  
André N. Dusi ◽  
César Carvalho ◽  
Antônio Carlos Torres ◽  
Antonio Carlos de Ávila
Keyword(s):  
Transgenic Plants ◽  
Optical Density ◽  
Potato Virus ◽  
Partial Resistance ◽  
Potato Virus Y ◽  
Systemic Infection ◽  
Transgenic Potato ◽  
Extreme Resistance ◽  
Density Values ◽  
Transgenic Potatoes

Two transgenic potato clones of cv. Achat, denominated 1P and 63P were challenged with two Potato virus Y strains (PVY O and PVY N), under greenhouse conditions, to be evaluated for resistance to these strains. Optical density values of the Elisa readings of samples from the transgenic plants were compared to readings from samples of the inoculated non-transformed plants. Clone 1P was extremely resistant to both PVY strains, reflected by not being systemically infected. Clone 63P, however, presented partial resistance to both PVY strains as local or systemic infection was delayed in some days. These results confirm the previously reported extreme resistance to PVY of clone 1P.

StPIP1, a PAMP-induced peptide in potato, elicits plant defenses and is associated with disease symptom severity in a compatible interaction with Potato virus Y

2021 ◽  
Author(s):  
Max M Combest ◽  
Natalia Moroz ◽  
Kiwamu Tanaka ◽  
Conner J Rogan ◽  
Jeffrey C Anderson ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Plant Defense ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Systemic Infection ◽  
Transgenic Potato ◽  
Defense Responses ◽  
Marker Genes ◽  
Plant Defense Responses ◽  
Callose Deposition

Abstract The role of small secreted peptides in plant defense responses to viruses has seldom been investigated. Here, we report a role for potato (Solanum tuberosum) PIP1, a gene predicted to encode a member of the pathogen-associated molecular pattern (PAMP)-induced peptide (PIP) family, in the response of potato to Potato virus Y (PVY) infection. We show that exogenous application of synthetic StPIP1 to potato leaves and nodes increased the production of reactive oxygen species and the expression of plant defense-related genes, revealing that StPIP1 triggers early defense responses. In support of this hypothesis, transgenic potato plants that constitutively overexpress StPIP1 had higher levels of leaf callose deposition and, based on measurements of viral RNA titers, were less susceptible to infection by a compatible PVY strain. Interestingly, systemic infection of StPIP1-overexpressing lines with PVY resulted in clear rugose mosaic symptoms that were absent or very mild in infected non-transgenic plants. A transcriptomics analysis revealed that marker genes associated with both pattern-triggered immunity and effector-triggered immunity were induced in infected StPIP1 overexpressors but not in non-transgenic plants. Together, our results reveal a role for StPIP1 in eliciting plant defense responses and in regulating plant antiviral immunity.

scholarly journals Potato Virus Y (PVY) Isolates from Solanum betaceum Represent Three Novel Recombinants Within the PVYN Strain Group and Are Unable to Systemically Spread in Potato

2020 ◽  
Vol 110 (9) ◽  
pp. 1588-1596
Author(s):  
Kelsie J. Green ◽  
Cassandra N. Funke ◽  
Jeffrey Chojnacky ◽  
Robert A. Alvarez-Quinto ◽  
Jose B. Ochoa ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Systemic Infection ◽  
Rt Pcr ◽  
Small Tree ◽  
Whole Genomes ◽  
Specific Strain ◽  
Slight Growth ◽  
Solanum Betaceum ◽  
Tree Tomato

Tamarillo, or tree tomato (Solanum betaceum), is a perennial small tree or shrub species cultivated in subtropical areas for fresh fruit and juice production. In Ecuador, tamarillo orchards are affected by several viruses, with one previously identified as potato virus Y (PVY); however, the specific strain composition of PVY in tamarillo was not determined. In 2015 and 2016, eight tamarillo plants exhibiting symptoms of leaf drop, mosaic, and mottled fruit were sampled near Tumbaco and Quito, Ecuador. These tamarillo PVY isolates were able to systemically infect tobacco, Nicotiana benthamiana, naranjilla, and tamarillo. Seven of the eight PVY isolates from tamarillo exhibited N-serotype, while one of the PVY isolates studied, Tam15, had no identifiable serotype. One isolate, Tam17, had N-serotype but produced asymptomatic systemic infection in tobacco. In tamarillo, four tamarillo isolates induced mosaic and slight growth retardation and were unable to systemically infect pepper or potato. Tamarillo, on the other hand, was unable to support systemic infection of PVY isolates belonging to the PVYO and PVYEu-N strains. The whole genomes of eight PVY isolates were sequenced from a series of overlapping RT-PCR fragments. Phylogenetically, tamarillo PVY isolates were found to belong to the large PVYN lineage, in a new tamarillo clade. Recombination analysis revealed that these tamarillo PVY isolates represent at least three novel recombinant types not reported before. The combination of the biological and molecular properties found in these eight PVY isolates suggested the existence of a new tamarillo strain of PVY that may have coevolved with S. betaceum.

scholarly journals A Model to Explain Temperature Dependent Systemic Infection of Potato Plants by Potato virus Y

2017 ◽  
Vol 33 (2) ◽  
pp. 206-211 ◽  
Author(s):  
Kyung San Choi ◽  
Francisco del Toro ◽  
Francisco Tenllado ◽  
Tomas Canto ◽  
Bong Nam Chung
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Systemic Infection ◽  
Temperature Dependent ◽  
Potato Plants

scholarly journals Resistance Response of the Recently Discovered Species Nicotiana mutabilis to Potato virus Y (PVY) and Tomato spotted wilt virus (TSWV) Compared to Other Sources of Resistance

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1617
Author(s):  
Anna Depta ◽  
Teresa Doroszewska ◽  
Anna Czubacka
Keyword(s):  
Potato Virus ◽  
Tomato Spotted Wilt Virus ◽  
Potato Virus Y ◽  
Susceptibility Gene ◽  
Systemic Infection ◽  
Sources Of Resistance ◽  
Resistance Response ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Das Elisa

Nicotiana mutabilis is a recently discovered species within the genus Nicotiana. The aim of the present study was to evaluate its resistance to Potato virus Y (PVY) and Tomato spotted wilt virus (TSWV). Molecular analysis was performed to detect the Va gene determining susceptibility to PVY and the SCAR marker associated with resistance to TSWV. Resistance tests were carried out under greenhouse conditions through artificial inoculation with one TSWV and two PVY isolates. In order to confirm the presence of the viruses in plants, DAS-ELISA tests were performed using antibodies against PVY and TSWV. The results indicated the absence of the PVY susceptibility gene and the presence of the TSWV resistance gene in the genome of N. mutabilis. This species was considered tolerant to the two PVY isolates tested because, despite the positive DAS-ELISA results, the infected plants showed vein clearing and chlorotic spots but no vein necrosis. As a result of TSWV inoculation, N. mutabilis showed a hypersensitive response; however, after four months, 30% of the inoculated plants showed systemic infection. This species extends the genetic variation in the genus Nicotiana and, because of its tolerance to PVY and partial resistance to TSWV, it may be a potential source of resistance to these viruses.

scholarly journals Resistance to potato virus A and potato virus Y in potato cultivars grown in Finland

1996 ◽  
Vol 5 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Jari P. T. Valkonen ◽  
Jukka P. Palohuhta
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Phenotypic Expression ◽  
Leaf Tissue ◽  
Systemic Infection ◽  
Potato Cultivars ◽  
Extreme Resistance ◽  
Hypersensitive Resistance ◽  
Potato Virus A ◽  
Das Elisa

Phenotypic expression of resistance to potato Y potyvirus (PVY) and potato A potyvirus (PVA) was tested in 24 potato cultivars and an advanced breeding line using graft-inoculation under controlled conditions in the glasshouse. Resistance phenotypes were determined based on symptom expression and systemic infection detected with DAS-ELISA. Tubers were harvested from the PVA-inoculated plants and tested for PVA with ELISA. Sixteen potato cultivars expressed hypersensitive resistance (HR) to the strain group YO of PVY. Ute expressed extreme resistance (ER) to PVY (strain groups YO and YN) and PVA, and eight cultivars (Amazone, Bintje, Fambo, Posmo, Record, Rosamunda, Saturna and Van Gogh) expressed ER to PVA. These cultivars produced no PVA-infected tubers (tubers of Record were not tested). Matilda and Nicola expressed HR to PVA. The tubers of graft-inoculated Matilda produced no PVA-infected shoots, whereas shoots from Nicola tubers developed necrosis and severe mosaic symptoms and were PVA-infected based on results from ELISA. Comparison with purified PVA antigen (using ELISA) indicated that the secondarily infected shoots of the 14 PVA-susceptible cultivars contained 206-804 ng of PVA antigen per gram of leaf tissue.

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