Establishment and characterization of rat cell lines transformed by restriction endonuclease fragments of adenovirus 12 DNA

Virology ◽  
1977 ◽  
Vol 82 (2) ◽  
pp. 462-471 ◽  
Author(s):  
Kazuko Shiroki ◽  
Hiroshi Handa ◽  
Hiroto Shimojo ◽  
Shoki Yano ◽  
Setsuko Ojima ◽  
...  
1984 ◽  
Vol 49 (1) ◽  
pp. 162-170 ◽  
Author(s):  
D T Rowe ◽  
P E Branton ◽  
S P Yee ◽  
S Bacchetti ◽  
F L Graham

2014 ◽  
Author(s):  
Naoto Nishimura ◽  
Saishu Yoshida ◽  
Masashi Higuchi ◽  
Hideji Yako ◽  
Hiroki Ueharu ◽  
...  
Keyword(s):  

2018 ◽  
Vol 69 (10) ◽  
pp. 2889-2894
Author(s):  
Ion Virgil Corlan ◽  
Adelina Cheveresan ◽  
Delia Berceanu Vaduva ◽  
Cristian Nica ◽  
Alin Faur ◽  
...  

The present study was aimed to evaluate the confluence percentage of three oral cell lines, namely primary gingival keratinocytes (PGK), primary gingival fibroblasts (HGF) and tongue squamous cell carcinoma (SCC-4). All cells have been monitored at different passages for 21 days. Evaluation of confluence percentage reveals the fact that primary gingival keratinocytes and tongue squamous cell carcinoma at small passages requires a period of about two weeks to reach a confluence of approximately 80% while for the gingival fibroblasts a period of about three times smaller is satisfactory.


Author(s):  
Youfeng Yang ◽  
Christopher J. Ricketts ◽  
Cathy D. Vocke ◽  
J. Keith Killian ◽  
Hesed M. Padilla‐Nash ◽  
...  

2013 ◽  
Vol 28 (3) ◽  
pp. 267-273 ◽  
Author(s):  
Marica Gemei ◽  
Rosa Di Noto ◽  
Peppino Mirabelli ◽  
Luigi Del Vecchio

In colorectal cancer, CD133+ cells from fresh biopsies proved to be more tumorigenic than their CD133– counterparts. Nevertheless, the function of CD133 protein in tumorigenic cells seems only marginal. Moreover, CD133 expression alone is insufficient to isolate true cancer stem cells, since only 1 out of 262 CD133+ cells actually displays stem-cell capacity. Thus, new markers for colorectal cancer stem cells are needed. Here, we show the extensive characterization of CD133+ cells in 5 different colon carcinoma continuous cell lines (HT29, HCT116, Caco2, GEO and LS174T), each representing a different maturation level of colorectal cancer cells. Markers associated with stemness, tumorigenesis and metastatic potential were selected. We identified 6 molecules consistently present on CD133+ cells: CD9, CD29, CD49b, CD59, CD151, and CD326. By contrast, CD24, CD26, CD54, CD66c, CD81, CD90, CD99, CD112, CD164, CD166, and CD200 showed a discontinuous behavior, which led us to identify cell type-specific surface antigen mosaics. Finally, some antigens, e.g. CD227, indicated the possibility of classifying the CD133+ cells into 2 subsets likely exhibiting specific features. This study reports, for the first time, an extended characterization of the CD133+ cells in colon carcinoma cell lines and provides a “dictionary” of antigens to be used in colorectal cancer research.


2013 ◽  
Vol 698 (1-3) ◽  
pp. 131-136 ◽  
Author(s):  
Frank Wunder ◽  
Annette Woermann ◽  
Andreas Geerts ◽  
Markus Milde

Sign in / Sign up

Export Citation Format

Share Document