Prostaglandin E2 production by renal inner medullary tissue slices: Effect of metabolic inhibitors

1977 ◽  
Vol 14 (4) ◽  
pp. 679-687 ◽  
Author(s):  
Ceil A. Herman ◽  
Terry V. Zenser ◽  
Bernard B. Davis
Keyword(s):  
Prostaglandin E2 ◽  
Metabolic Inhibitors ◽  
Tissue Slices ◽  
Medullary Tissue

scholarly journals The Physiology of Sugar-Cane IV. Effects of Inhibitors on Sugar Accumulation in Storage Tissue Slices

1960 ◽  
Vol 13 (3) ◽  
pp. 221 ◽  
Author(s):  
RL Bieleski
Keyword(s):  
Sugar Cane ◽  
Metabolic Inhibitors ◽  
Magnesium Ion ◽  
Sugar Accumulation ◽  
Tissue Slices ◽  
Storage Tissue ◽  
Cupric Ion ◽  
Mercuric Ion ◽  
Rate Of Accumulation

Various metabolic inhibitors, at pH 5� 5, affected sugar accumulation in immature sugar-cane storage tissues. The rate of accumulation was reduced by lO-5M mercuric ion, lO-<M p-chloromercuribenzoate, cyanide, and cupric ion, and 2 X lO-3M phloridzin. Accumulation was completely inhibited and sugar leakage induced by lO-5M dinitrophenol, lO-<M mercuric ion, and 10-3M p-chloromercuribenzoate, cyanide, cupric ion, azide, arsenate, and iodoacetate. The effects of 10-5M dinitrophenol and 10-4M cyanide were reversible, but that of 10-3M cyanide was irreversible. Only slight effects were produced by borate, phosphate, and magnesium ion.

Effects of endogenous pyrogen and prostaglandin E2 on hypothalamic neurons in guinea pig brain slices

1987 ◽  
Vol 63 (1) ◽  
pp. 175-180 ◽  
Author(s):  
T. Ono ◽  
A. Morimoto ◽  
T. Watanabe ◽  
N. Murakami
Keyword(s):  
Prostaglandin E2 ◽  
Neuronal Activity ◽  
Neuronal Response ◽  
Brain Slices ◽  
Direct Effects ◽  
Endogenous Pyrogen ◽  
Tissue Slices ◽  
Thermoresponsive Neurons ◽  
The Individual ◽  
Guinea Pig Brain

To investigate the direct effects of endogenous pyrogen (EP) and prostaglandin E2 (PGE2) on the activity of neurons in the preoptic and anterior hypothalamic (PO-AH) region, single-unit activity was recorded from brain tissue slices prepared from the PO-AH region of guinea pigs. When EP was applied into the perfusate 18% of warm-responsive neurons decreased their activity, and 23% of warm-responsive neurons increased their activity. Most of the thermally insensitive neurons did not respond to EP. PGE2 inhibited 29% of warm-responsive neurons and facilitated 15% of them. Moreover, when EP and PGE2 were applied to the same neurons at different times, the same directions of changes in neuronal activity were observed in 72% of total neurons examined. These results suggest that EP and PGE2 change the neuronal activity of the thermoresponsive neurons in the PO-AH region involved in fever induction. However, by these results, the direction of neuronal response induced by these substances could not be generally categorized based on the thermoresponsiveness of the individual neuron.

In vitro effects of various metabolic inhibitors on the formation of inactive renin and the loss of renin in rabbit uterine tissue

1981 ◽  
Vol 96 (2) ◽  
pp. 281-288 ◽  
Author(s):  
Jørgen Jørgensen
Keyword(s):  
Energy Metabolism ◽  
De Novo ◽  
Post Partum ◽  
Metabolic Inhibitors ◽  
Kidney Cortex ◽  
Uterine Tissue ◽  
Tissue Slices ◽  
Inactive Renin ◽  
A Minor

Abstract. A pronounced formation of renin occurs during incubation of non-pregnant uterine tissue slices in vitro. The synthesized renin appears in an enzymatically inactive form, which can be activated by acidification. Prior to incubation only a small fraction of inactive renin is present. The formation of inactive renin is blocked by puromycin and by inhibition of energy metabolism, indicating a de novo synthesis. A similar pattern of inhibition prevails the modest formation of inactive renin in post-partum uterus. The marked loss of active renin seen during incubation of post-partum uterine tissue is partly prevented by an inhibition of energy metabolism. Potent inhibitors are iodoacetate and chloroquine. These findings are in accordance with lysosomal engagement in the inactivation of renin. Incubated kidney cortex tissue shows only a minor loss of renin during incubation. This loss is uninfluenced by attempts to block it.

Effect of Some Metabolic Inhibitors on Vinblastine-Induced Ribosomal-Granular Material Complexes

1971 ◽  
Vol 29 ◽  
pp. 548-549
Author(s):  
Awtar Krishan ◽  
Dora Hsu
Keyword(s):  
Granular Material ◽  
Rna Synthesis ◽  
Culture Medium ◽  
Actinomycin D ◽  
Metabolic Inhibitors ◽  
Protein And Rna Synthesis ◽  
Apparent Reduction ◽  
Plant Alkaloids ◽  
In Cells

Cells exposed to antitumor plant alkaloids, vinblastine and vincristine sulfate have large proteinacious crystals and complexes of ribosomes, helical polyribosomes and electron-dense granular material (ribosomal complexes) in their cytoplasm, Binding of H3-colchicine by the in vivo crystals shows that they contain microtubular proteins. Association of ribosomal complexes with the crystals suggests that these structures may be interrelated.In the present study cultured human leukemic lymphoblasts (CCRF-CEM), were incubated with protein and RNA-synthesis inhibitors, p. fluorophenylalanine, puromycin, cycloheximide or actinomycin-D before the addition of crystal-inducing doses of vinblastine to the culture medium. None of these compounds could completely prevent the formation of the ribosomal complexes or the crystals. However, in cells pre-incubated with puromycin, cycloheximide, or actinomycin-D, a reduction in the number and size of the ribosomal complexes was seen. Large helical polyribosomes were absent in the ribosomal complexes of cells treated with puromycin, while in cells exposed to cycloheximide, there was an apparent reduction in the number of ribosomes associated with the ribosomal complexes (Fig. 2).

Automated 3-D cell population analysis in thick tissue sections using laser-scanning confocal-microscopy data

1993 ◽  
Vol 51 ◽  
pp. 562-563
Author(s):  
Hakan Ancin
Keyword(s):  
Laser Scanning ◽  
Population Analysis ◽  
Three Dimensional ◽  
Large Data ◽  
Laser Scanning Confocal Microscopy ◽  
Tissue Slices ◽  
Scanning Confocal Microscopy ◽  
Tissue Sections ◽  
Spatially Adaptive ◽  
Microscopy Data

This paper presents methods for performing detailed quantitative automated three dimensional (3-D) analysis of cell populations in thick tissue sections while preserving the relative 3-D locations of cells. Specifically, the method disambiguates overlapping clusters of cells, and accurately measures the volume, 3-D location, and shape parameters for each cell. Finally, the entire population of cells is analyzed to detect patterns and groupings with respect to various combinations of cell properties. All of the above is accomplished with zero subjective bias.In this method, a laser-scanning confocal light microscope (LSCM) is used to collect optical sections through the entire thickness (100 - 500μm) of fluorescently-labelled tissue slices. The acquired stack of optical slices is first subjected to axial deblurring using the expectation maximization (EM) algorithm. The resulting isotropic 3-D image is segmented using a spatially-adaptive Poisson based image segmentation algorithm with region-dependent smoothing parameters. Extracting the voxels that were labelled as "foreground" into an active voxel data structure results in a large data reduction.

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