Localization of serum-derived α2 macroglobulin in cultured cells and decrease after moloney sarcoma virus transformation

Cell ◽  
1977 ◽  
Vol 12 (3) ◽  
pp. 609-617 ◽  
Author(s):  
Ira Pastan ◽  
Mark Willingham ◽  
Wayne Anderson ◽  
Maria Gallo
Keyword(s):  
Cultured Cells ◽  
Α2 Macroglobulin ◽  
Sarcoma Virus ◽  
Moloney Sarcoma Virus ◽  
Virus Transformation

Cellular Moloney murine sarcoma (c-mos) sequences are hypermethylated and transcriptionally silent in normal and transformed rodent cells

1982 ◽  
Vol 2 (1) ◽  
pp. 42-51
Author(s):  
S Gattoni ◽  
P Kirschmeier ◽  
I B Weinstein ◽  
J Escobedo ◽  
D Dina
Keyword(s):  
Cell Lines ◽  
Blot Hybridization ◽  
Cell Types ◽  
Single Copy ◽  
Transformed Cell Line ◽  
Murine Sarcoma Virus ◽  
Sarcoma Virus ◽  
Blotting Technique ◽  
Moloney Sarcoma Virus ◽  
Murine Sarcoma

Moloney murine sarcoma virus carries an oncogenic sequence (v-mos) which is homologous to a single copy gene (c-mos) present in the normal cells of several vertebrate species. Because of the possible significance of c-mos sequences in normal development and malignant transformation induced by physical or chemical agents, we have examined the state of integration, methylation, and transcriptional activity of c-mos sequences in a variety of normal rodent tissues, normal cell lines, or cell lines transformed by radiation or chemical carcinogens. DNA-DNA hybridization, utilizing the Southern blotting technique and a plasmid-derived DNA probe representing the v-mos sequence, gave no evidence for rearrangements of the c-mos sequence in the DNAs obtained from these diverse cell types. Parallel studies employing the restriction enzyme isoschizomers HpaII and MspI indicated that in all of these cell types the c-mos sequences were heavily methylated. In addition, analysis of cellular RNAs by blot hybridization with the v-mos probe failed to detect evidence of transcription of the c-mos sequences in any of these cell types. This was in contrast to a Moloney sarcoma virus-transformed cell line in which we found that the integrated v-mos sequence was both undermethylated and extensively transcribed. Thus, it would appear that c-mos sequences do not play a role in the transformation of rodent cells by chemical or physical agents, although the possible role of other endogenous onc sequences remains to be determined.

Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells

1984 ◽  
Vol 4 (12) ◽  
pp. 2929-2931 ◽  
Author(s):  
K Blochlinger ◽  
H Diggelmann
Keyword(s):  
Selectable Marker ◽  
Dna Transfer ◽  
Direct Selection ◽  
Regulatory Sequences ◽  
Hygromycin B ◽  
Dominant Marker ◽  
Dna Coding ◽  
Sarcoma Virus ◽  
Moloney Sarcoma Virus ◽  
Selection For

The DNA coding sequence for the hygromycin B phosphotransferase gene was placed under the control of the regulatory sequences of a cloned long terminal repeat of Moloney sarcoma virus. This construction allowed direct selection for hygromycin B resistance after transfection of eucaryotic cell lines not naturally resistant to this antibiotic, thus providing another dominant marker for DNA transfer in eucaryotic cells.

scholarly journals Heat shock induces protein tyrosine phosphorylation in cultured cells.

1989 ◽  
Vol 108 (6) ◽  
pp. 2029-2035 ◽  
Author(s):  
P A Maher ◽  
E B Pasquale
Keyword(s):  
Heat Shock ◽  
Tyrosine Phosphorylation ◽  
Cultured Cells ◽  
Protein Tyrosine Phosphorylation ◽  
Animal Cells ◽  
Protein Tyrosine ◽  
Rous Sarcoma ◽  
Wide Range ◽  
Sarcoma Virus ◽  
Molecular Masses

We examined the effect of heat shock on protein tyrosine phosphorylation in cultured animal cells using antiphosphotyrosine antibodies in immunoblotting and immunofluorescence microscopy experiments. Heat shock significantly elevated the level of phosphotyrosine in proteins in most of the cultured cells examined, including fibroblasts, epithelial cells, nerve cells, and muscle cells, but not in Rous sarcoma virus-transformed fibroblasts. The increase in protein tyrosine phosphorylation induced by heat shock occurred in proteins with a wide range of molecular masses and was dependent on the temperature and duration of the heat shock.

scholarly journals THE EFFECT OF RIFAMPICIN, AND TWO DERIVATIVES, ON CELLS INFECTEDWITH MOLONEY SARCOMA VIRUS

1971 ◽  
Author(s):  
Melvin. Calvin ◽  
Urs R. Joss ◽  
Adeline J. Hackett ◽  
RobertB. Owens
Keyword(s):  
Sarcoma Virus ◽  
Moloney Sarcoma Virus
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