The cycle of the seminiferous epithelium in the Australian Swamp buffalo

Classically, the male germinal epithelium is depicted as synchronously developing uninucleate spermatids conjoined by intercellular bridges. Recently, binucleate and multinucleate spermatids from human and mouse testis have been reported. The present paper describes certain developmental events in one type of binucleate spermatid in the seminiferous epithelium of the mouse.Testes of adult mice (ABP Jax) were removed from the animals after cervical dislocation and placed into 2.5% glutaraldehyde/Millonig's phosphate buffer (pH 7.2). Testicular capsules were gently split and separated, exposing the tubules. After 15 minutes the tissue was carefully cut into cubes (approx. 1mm), fixed for an additional 45 minutes and processed for electron microscopy.

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Immunodistribution of RFamide-related peptide-3 (RFRP-3) during the seminiferous epithelium cycle in a desert rodent Psammomys obesus

Tissue and Cell ◽

10.1016/j.tice.2020.101484 ◽

2020 ◽

pp. 101484

Author(s):

Nassima Djouahra ◽

Elara N. Moudilou ◽

Jean-Marie Exbrayat ◽

Sadjia Hammouche

Keyword(s):

Seminiferous Epithelium ◽

Psammomys Obesus ◽

Related Peptide ◽

Desert Rodent

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Differential expression of gap-junction gene connexin 31 in seminiferous epithelium of rat testes

FEBS Letters ◽

10.1016/s0014-5793(99)00714-0 ◽

1999 ◽

Vol 453 (3) ◽

pp. 243-248 ◽

Cited By ~ 24

Author(s):

Bobo W Mok ◽

William S.B Yeung ◽

John M Luk

Keyword(s):

Gap Junction ◽

Differential Expression ◽

Seminiferous Epithelium

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Expression of beta-nerve growth factor and its receptor in rat seminiferous epithelium: specific function at the onset of meiosis.

The Journal of Cell Biology ◽

10.1083/jcb.117.3.629 ◽

1992 ◽

Vol 117 (3) ◽

pp. 629-641 ◽

Cited By ~ 63

Author(s):

M Parvinen ◽

M Pelto-Huikko ◽

O Söder ◽

R Schultz ◽

A Kaipia ◽

...

Keyword(s):

Plasma Membrane ◽

Nerve Growth Factor ◽

Growth Factor ◽

Sertoli Cells ◽

Seminiferous Epithelium ◽

Receptor Protein ◽

Tyrosine Kinase Receptor ◽

Specific Expression ◽

Nerve Growth ◽

Ngf Receptor

beta-Nerve growth factor (NGF) is expressed in spermatogenic cells and has testosterone-downregulated low-affinity receptors on Sertoli cells suggesting a paracrine role in the regulation of spermatogenesis. An analysis of the stage-specific expression of NGF and its low affinity receptor during the cycle of the seminiferous epithelium in the rat revealed NGF mRNA and protein at all stages of the cycle. Tyrosine kinase receptor (trk) mRNA encoding an essential component of the high-affinity NGF receptor was also present at all stages. In contrast, expression of low affinity NGF receptor mRNA was only found in stages VIIcd and VIII of the cycle, the sites of onset of meiosis. The low-affinity NGF receptor protein was present in the plasma membrane of the apical Sertoli cell processes as well as in the basal plasma membrane of these cells at stages VIIcd to XI. NGF was shown to stimulate in vitro DNA synthesis of seminiferous tubule segments with preleptotene spermatocytes at the onset of meiosis while other segments remained nonresponsive. We conclude that NGF is a meiotic growth factor that acts through Sertoli cells.

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