Methyl isocyanate induced morphological changes in the seminiferous epithelium of rats maintained on normal or protein deficient diets

1994 ◽  
Vol 52 (5) ◽  
Author(s):  
M. Bose ◽  
K.D. Vachhrajani ◽  
B.S. Jha ◽  
K.K. Dutta
Keyword(s):  
Morphological Changes ◽  
Seminiferous Epithelium ◽  
Methyl Isocyanate

scholarly journals Spermiogenesis, Stages of Seminiferous Epithelium and Variations in Seminiferous Tubules during Active States of Spermatogenesis in Yangzhou Goose Ganders

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 570
Author(s):  
Muhammad Faheem Akhtar ◽  
Ejaz Ahmad ◽  
Sheeraz Mustafa ◽  
Zhe Chen ◽  
Zhendan Shi ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Morphological Changes ◽  
Seminiferous Epithelium ◽  
Poultry Meat ◽  
Seminiferous Tubules ◽  
Germplasm Resources ◽  
High Productivity ◽  
The Past ◽  
The World ◽  
Tubular Diameter

The past three decades revolutionized the goose industry in the world. China holds the world’s largest goose breeds stock by 95% of the global total. To optimize the goose industry and cope with ever increasing poultry meat and egg demands, there is a dire need to focus on reproduction, as most geese breeds exhibit poor reproductive performance. The present study was conducted with the aim to add a contribution in the goose industry and research by the histological visualizing step wise development of germ cells during spermatogenesis by microscopy and a histological technique. Yangzhou goose is a synthetic breed developed by using local goose germplasm resources of China. It is popular in the Chinese goose industry due to high productivity and adaptability. This research evaluated the steps of spermiogenesis and stages along with morphological changes in the seminiferous epithelium in Yangzhou goose ganders. For the assessment of various stages of the seminiferous epithelium cycle, testis sections were embedded in molten paraffin wax. The initial steps of spermiogenesis were depicted by changes in acrosomic granules, whereas further stages were identified by nuclear morphological changes. Ten steps of spermiogenesis and nine stages of seminiferous epithelium were identified. Four types of spermatogonia Ad, Ap1, Ap2 and B were recognized. The results depicted a clear variation in the diameter of seminiferous tubules (ST), epithelium height (EH), luminal tubular diameter (LD), number of seminiferous tubules per field and the Johnsen score. Microscopy indicated that the stages of seminiferous epithelium were similar to other birds and mammals and the ST diameter, EH, LD and Johnsen score are positively correlated while the number of seminiferous tubules per field is negatively correlated with the ST diameter, EH, LD and Johnsen score. Fertility in Yangzhou ganders can further be improved by visualizing the histological development of germs cells in testis tissues during spermatogenesis after onset of breeding season and maturity. Our results suggest that Yangzhou ganders reach complete sexual maturity at 227 days of age.

Spermatogenesis recovery in the mouse after iron injury

2003 ◽  
Vol 22 (5) ◽  
pp. 275-279 ◽  
Author(s):  
Maria De Lourdes Pereira ◽  
Fernando Garciae Costa
Keyword(s):  
Sertoli Cells ◽  
Morphological Changes ◽  
Recovery Period ◽  
Seminiferous Epithelium ◽  
Seminiferous Tubules ◽  
Prosthetic Devices ◽  
Transmission Electron ◽  
Normal Functional ◽  
Iron Nickel ◽  
Cytological Features

Alloys used as prosthetic devices for bone/joint replacement include some heavy metals such as chromium, iron, nickel, or titanium. Unfortunately, due to the aggressive nature of the physiological environment, corrosion of these alloys promotes the release of metal ions into the surrounding tissues causing systemic toxic effects. Our previous preliminary studies have demonstrated that iron induced several morphological changes within mice seminiferous epithelium. The aim of the present work was to investigate, over a one-month period, the possibility of recovery of mice seminiferous epithelium, previously damaged by iron. Male Charles River mice were dosed subcutaneously with 0.5 mL of an iron suspension of 538 mg/L9-10 10 mg/L (n-5) every 72 hours during two weeks, followed by a recovery period of 30 days. Fragments of the seminiferous tubules were fixed in glutaraldehyde and prepared for light and transmission electron microscopy. Regeneration of spermatogenesis was noted after a one-month period, as illustrated by the presence of normal germ cells, in the usual position within the seminiferous tubules. These germinal elements and the Sertoli cells have shown normal cytological features. These results strongly suggest that the deleterious effects induced by iron are reversible. The presence of residual bodies within Sertoli cells cytoplasm indicates that they are able to perform a normal functional activity in a recovered spermatogenesis.

scholarly journals 14-3-3 and its binding partners are regulators of protein–protein interactions during spermatogenesis

2009 ◽  
Vol 202 (3) ◽  
pp. 327-336 ◽  
Author(s):  
Shengyi Sun ◽  
Elissa W P Wong ◽  
Michelle W M Li ◽  
Will M Lee ◽  
C Yan Cheng
Keyword(s):  
Cell Adhesion ◽  
Protein Interactions ◽  
Protein Complexes ◽  
Morphological Changes ◽  
Regulatory Protein ◽  
Cell Movement ◽  
Cytoskeletal Proteins ◽  
Seminiferous Epithelium ◽  
Protein Protein Interactions ◽  
Binding Partners

During spermatogenesis, spermiation takes place at the adluminal edge of the seminiferous epithelium at stage VIII of the epithelial cycle during which fully developed spermatids (i.e. spermatozoa) detach from the epithelium in adult rat testes. This event coincides with the migration of preleptotene/leptotene spermatocytes across the blood–testis barrier from the basal to the apical (or adluminal) compartment. At stage XIV of the epithelial cycle, Pachytene spermatocytes (diploid, 2n) differentiate into diplotene spermatocytes (tetraploid, 4n) in the apical compartment of the epithelium, which begin meiosis I to be followed by meiosis II to form spermatids (haploid, 1n) at stage XIV of the epithelial cycle. These spermatids, in turn, undergo extensive morphological changes and traverse the seminiferous epithelium until they differentiate into elongated spermatids. Thus, there are extensive changes at the Sertoli–Sertoli and Sertoli–germ cell interface via protein ‘coupling’ and ‘uncoupling’ between cell adhesion protein complexes, as well as changes in interactions between integral membrane proteins and their peripheral adaptors, regulatory protein kinases and phosphatases, and the cytoskeletal proteins. These precisely coordinated protein–protein interactions affect cell adhesion and cell movement. In this review, we focus on the 14-3-3 protein family, whose members have different binding partners in the seminiferous epithelium. Recent studies have illustrated that 14-3-3 affects protein–protein interactions in the seminiferous epithelium, and regulates cell adhesion possibly via its effects on intracellular protein trafficking and cell-polarity proteins. This review provides a summary on the latest findings regarding the role of 14-3-3 family of proteins and their potential implications on spermatogenesis. We also highlight research areas that deserve attentions by investigators.

scholarly journals THE EFFECTS OF CHRONIC STRESS ON THE MORPHOLOGICAL CHARACTERISTICS OF TESTICULAR TISSUES OF WISTAR RATS

2017 ◽  
pp. 66-69
Author(s):  
K. A. Kidun ◽  
E. K. Solodova ◽  
T. S. Ugolnik
Keyword(s):  
Basement Membrane ◽  
Chronic Stress ◽  
Reproductive System ◽  
Wistar Rats ◽  
Morphological Changes ◽  
Morphological Characteristics ◽  
Male Reproductive System ◽  
Seminiferous Epithelium ◽  
Seminiferous Tubules ◽  
Unfavorable Effect

Stress has an unfavorable effect on male reproductive system. The aim of the work was to study the morphological changes in testicular tissues of Wistar rats in chronic stress according to Ortiz. We detected hyperemia of vessels, reduction of the diameter of seminiferous tubules, exfoliation of the seminiferous epithelium from the basement membrane, and also destructive changes in the seminiferous epithelium including development of necrosis in the testis of the rats after stress.

Morphological Changes in the Rat Granulosa Cell Surface During Differentiation Induced by Estradiol and Gonadotropins

1977 ◽  
Vol 35 ◽  
pp. 484-485
Author(s):  
P. Bagavandoss ◽  
JoAnne S. Richards ◽  
A. Rees Midgley
Keyword(s):  
Cell Surface ◽  
Granulosa Cell ◽  
Morphological Changes ◽  
Follicular Development ◽  
Female Rats ◽  
Functional Changes ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

During follicular development in the mammalian ovary, several functional changes occur in the granulosa cells in response to steroid hormones and gonadotropins (1,2). In particular, marked changes in the content of membrane-associated receptors for the gonadotropins have been observed (1).We report here scanning electron microscope observations of morphological changes that occur on the granulosa cell surface in response to the administration of estradiol, human follicle stimulating hormone (hFSH), and human chorionic gonadotropin (hCG).Immature female rats that were hypophysectcmized on day 24 of age were treated in the following manner. Group 1: control groups were injected once a day with 0.1 ml phosphate buffered saline (PBS) for 3 days; group 2: estradiol (1.5 mg/0.2 ml propylene glycol) once a day for 3 days; group 3: estradiol for 3 days followed by 2 days of hFSH (1 μg/0.1 ml) twice daily, group 4: same as in group 3; group 5: same as in group 3 with a final injection of hCG (5 IU/0.1 ml) on the fifth day.

Effect of verapamil on cytoplasmic distribution of granules and microfilaments in amphibian urinary bladder

1988 ◽  
Vol 46 ◽  
pp. 324-325
Author(s):  
A.J. Mia ◽  
L.X. Oakford ◽  
T. Yorio
Keyword(s):  
Urinary Bladder ◽  
Water Flow ◽  
Morphological Changes ◽  
Granular Cell ◽  
Cytosolic Calcium ◽  
Calcium Storage ◽  
Amphibian Urinary Bladder ◽  
Vesicular Membrane ◽  
High Concentrations ◽  
Cytoskeletal System

The amphibian urinary bladder has been used as a ‘model’ system for studies of the mechanism of action of antidiuretic hormone (ADH) in stimulating transepithelial water flow. The increase in water permeability is accompanied by morphological changes that include the stimulation of apical microvilli, mobilization of microtubules and microfilaments and vesicular membrane fusion events . It has been shown that alterations in the cytosolic calcium concentrations can inhibit ADH transmembrane water flow and induce alterations in the epithelial cell cytomorphology, including the cytoskeletal system . Recently, the subapical granules of the granular cell in the amphibian urinary bladder have been shown to contain high concentrations of calcium, and it was suggested that these cytoplasmic constituents may act as calcium storage sites for intracellular calcium homeostasis. The present study utilizes the calcium antagonist, verapamil, to examine the effect of calcium deprivation on the cytomorphological features of epithelial cells from amphibian urinary bladder, with particular emphasis on subapical granule and microfilament distribution.

Binucleate Spermiogenesis in the Mouse

1972 ◽  
Vol 30 ◽  
pp. 112-113
Author(s):  
John J. Wolosewick
Keyword(s):  
Electron Microscopy ◽  
Phosphate Buffer ◽  
Seminiferous Epithelium ◽  
Mouse Testis ◽  
Germinal Epithelium ◽  
Intercellular Bridges ◽  
Adult Mice ◽  
Cervical Dislocation ◽  
Human And Mouse

Classically, the male germinal epithelium is depicted as synchronously developing uninucleate spermatids conjoined by intercellular bridges. Recently, binucleate and multinucleate spermatids from human and mouse testis have been reported. The present paper describes certain developmental events in one type of binucleate spermatid in the seminiferous epithelium of the mouse.Testes of adult mice (ABP Jax) were removed from the animals after cervical dislocation and placed into 2.5% glutaraldehyde/Millonig's phosphate buffer (pH 7.2). Testicular capsules were gently split and separated, exposing the tubules. After 15 minutes the tissue was carefully cut into cubes (approx. 1mm), fixed for an additional 45 minutes and processed for electron microscopy.

Hydrated form of some clay minerals observed using a film sealed environmental cell

1978 ◽  
Vol 36 (1) ◽  
pp. 72-73
Author(s):  
N. Kohyama ◽  
K. Fukushima ◽  
A. Fukami
Keyword(s):  
Clay Minerals ◽  
Morphological Changes ◽  
Carbon Film ◽  
Electron Microscopic Observation ◽  
Adsorbed Water ◽  
Electron Microscopic ◽  
Hydrated Form ◽  
Thin Carbon ◽  
Environmental Cell ◽  
Thin Carbon Film

Since the interlayer or adsorbed water of some clay minerals are quite easily dehydrated in dried air, in vacuum, or at moderate temperatures even in the atmosphere, the hydrated forms have not been observed by a conventional electron microscope(TEM). Recently, specific specimen chambers, “environmental cells(E.C.),” have been developed and confirmed to be effective for electron microscopic observation of wet specimen without dehydration. we observed hydrated forms of some clay minerals and their morphological changes by dehydration using a TEM equipped with an E.C..The E.C., equipped with a single hole copper-microgrid sealed by thin carbon-film, attaches to a TEM(JEM 7A) with an accelerating voltage 100KV and both gas pressure (from 760 Torr to vacuum) and relative humidity can be controlled. The samples collected from various localities in Japan were; tubular halloysite (l0Å) from Gumma Prefecture, sperical halloysite (l0Å) from Tochigi Pref., and intermediate halloysite containing both tubular and spherical types from Fukushima Pref..

Ultrastructural Study of Rat Colon Following Psyllium Ingestion

1985 ◽  
Vol 43 ◽  
pp. 580-581
Author(s):  
F.G. Lightfoot ◽  
L.E. Grau ◽  
M.M. Cassidy ◽  
G.R. Tadvalkar ◽  
G.V. Vahouny
Keyword(s):  
Electron Microscopy ◽  
Morphological Changes ◽  
Large Population ◽  
Rat Colon ◽  
Gastrointestinal Disorders ◽  
Luminal Surface ◽  
Fecal Material ◽  
Transmission Electron ◽  
Morphologic Analysis ◽  
Irritable Bowel

Psyllium hydrophillic mucilloid is a natural gelling fiber consumed by a large population of our society. It is used as a bulk-producing laxative and in the treatment of gastrointestinal disorders such as “Irritable Bowel Syndrome”. The literature pertaining to the ultrastructural effects of this agent is sparse.This study documents morphological changes induced by psyllium. Animals fed a diet containing 2% psyllium for four weeks were subsequently sacrificed and processed for scanning and transmission electron microscopy. The colon contained fecal material combined with psyllium which conformed to the contour of the luminal surface. This mixture formed surface replicas of the intestinal mucosa. These replicas and their related colonic sites were processed for morphologic analysis.

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