scholarly journals Passive Immunization with Anti-Laminin Immunoglobulin G Modifies the Integrity of the Seminiferous Epithelium and Induces Arrest of Spermatogenesis in the Guinea Pig1

2000 ◽  
Vol 62 (6) ◽  
pp. 1505-1514 ◽  
Author(s):  
Livia Lustig ◽  
Berta Denduchis ◽  
Roberto Ponzio ◽  
Michel Lauzon ◽  
R.-Marc Pelletier
Keyword(s):  
Immunoglobulin G ◽  
Passive Immunization ◽  
Seminiferous Epithelium

scholarly journals Colostrum composition and immunoglobulin G content in dairy and dual-purpose cattle breeds

2020 ◽  
Vol 98 (8) ◽  
Author(s):  
Evelyne C Kessler ◽  
Rupert M Bruckmaier ◽  
Josef J Gross
Keyword(s):  
Dairy Cows ◽  
Immunoglobulin G ◽  
Passive Immunization ◽  
Mature Milk ◽  
Dual Purpose ◽  
Cattle Breeds ◽  
Holstein Friesian ◽  
Essential Components ◽  
Dual Purpose Cattle ◽  
And Performance

Abstract Immunoglobulins (Ig) are essential components in the colostrum of bovine species that enable passive immunization of newborn calves. Concentrations of fat and protein are greater in colostrum compared with mature milk and represent a vital source of energy and nutrients. Colostral IgG was shown to vary between individual dairy cows, but comparative data on different breeds and performance levels are scarce. The objective of the present field study was to investigate the contents of total IgG, fat, protein, and lactose in colostrum in different Swiss and German dairy and dual-purpose breeds. We collected colostrum samples of 458 cows of 13 different breeds (dairy breeds: Brown Swiss, Swiss and German Holstein Friesian, and New Zealand Holstein; dual-purpose breeds: German Fleckvieh, Holstein Friesian × Montbéliarde, Montbéliarde, Murnau-Werdenfels, Original Braunvieh, Pinzgauer, Rhetic Gray, and Simmental; and beef-type crossbred: Charolais × Holstein Friesian). Colostrum samples were obtained between 5 and 900 min after calving and analyzed for total IgG, fat protein, and lactose contents. Immunoglobulin G concentrations varied between 12.7 and 204.0 mg/mL. No effect of breeding purpose (i.e., dairy or dual-purpose) nor of previous lactation yield on IgG content was observed. However, milking of cows for the first time later than 12 h after parturition resulted in lower colostrum IgG concentrations compared with colostrum harvest within 9 h after calving (P < 0.05). Multiparous cows had a higher colostral IgG concentration than primiparous cows (P < 0.0001). Overall, concentrations of IgG and other constituents in colostrum varied widely in the different cattle breeds. High-yielding dairy cows did not have poorer colostrum quality compared with lower-yielding animals or beef and dual-purpose breeds, which suggests an individually different transfer of circulating IgG into colostrum.

scholarly journals Ovarian Localization of Immunoglobulin G and Inhibin .ALPHA.-Subunit in Guinea Pigs after Passive Immunization against the Inhibin .ALPHA.-Subunit.

2000 ◽  
Vol 46 (5) ◽  
pp. 293-299 ◽  
Author(s):  
FangXiong SHI ◽  
Keiji MOCHIDA ◽  
Osamu SUZUKI ◽  
Junichiro MATSUDA ◽  
Atsuo OGURA ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Guinea Pigs ◽  
Passive Immunization ◽  
Alpha Subunit

scholarly journals Colostrum production of Alentejano and Large-White × Landrace sows: consumption, passive immunity and mortality of piglets

2015 ◽  
Vol 13 (4) ◽  
pp. e0611 ◽  
Author(s):  
Rui Charneca ◽  
Maria J. Vila-Viçosa ◽  
Paulo Infante ◽  
José Nunes ◽  
Jean Le Dividich
Keyword(s):  
Birth Weight ◽  
Mortality Rate ◽  
Immunoglobulin G ◽  
Passive Immunization ◽  
Passive Immunity ◽  
Large White ◽  
Factors Influencing ◽  
Main Factors ◽  
Piglet Mortality ◽  
The Difference

<p>Colostrum production and composition of 45 Alentejano (AL) and 45 crossed (Large–White × Landrace, LL) sows together with consumption and passive immunization of piglets, were evaluated. Piglets were weighed at birth, 24h, and 21 and 28 days of age. Colostrum was collected, chemically analysed and immunoglobulin G (IgG) determined. On piglets, serum IgG concentrations were determined at 2d and 28d of age. Alentejano piglets consumed 19% less colostrum than LL piglets (267 <em>vs</em> 328 g; <em>p</em>&lt;0.001), but when adjusted for birth weight, the difference between genotypes was not significant (<em>p</em>=0.891). Alentejano sows produced less colostrum (1985±139 g <em>vs</em> 3761±139 g, <em>p</em>&lt;0.001) than LL sows, even after adjustment for litter birth weight. IgG concentrations were higher in AL colostrum at 12 h and 36 h after the onset of farrowing. They were higher in AL than in LL piglets (<em>p</em>=0.025) and higher at 2d than at 28d of age (<em>p&lt;</em>0.001). Mortality rate of piglets until d21 post–farrowing was higher in AL genotype (27% <em>vs</em> 16.1%, <em>p</em>&lt;0.001). In both genotypes main factors influencing piglet mortality were birth weight and colostrum intake (g/kg birth weight). Higher mortality in AL than in LL genotype was attributable to the higher proportion (28.7% <em>vs</em> 11.6%, <em>p</em>&lt;0.001) of light piglets. It is concluded that for similar birth weight, both AL and LL piglets have a similar ability to ingest colostrum and that AL sows are less able to produce colostrum than LL sows. IgG concentrations of colostrum and of piglets serum were higher in AL than in LL genotype.</p>

Passive immunization against progesterone inhibits endometrial sensitization in pseudopregnant mice and has antifertility effects in pregnant mice which are reversible by steroid treatment

1985 ◽  
Vol 104 (1) ◽  
pp. 153-158 ◽  
Author(s):  
V. Rider ◽  
A. McRae ◽  
R. B. Heap ◽  
A. Feinstein
Keyword(s):  
Monoclonal Antibody ◽  
Immunoglobulin G ◽  
Passive Immunization ◽  
Steroid Treatment ◽  
Uterine Horn ◽  
Decidual Cell ◽  
Cell Reaction ◽  
Antibody Treatment ◽  
Pregnant Mice ◽  
Implantation Sites

ABSTRACT Pregnant mice were injected 32 h post coitum (p.c.) with a monoclonal antibody against progesterone (5·7 or 9·5 nmol immunoglobulin G (IgG)) or 0·9% (w/v) NaCl (controls). Progesterone was injected starting on day 2, 3, 4 or 5 p.c. Progesterone reversed the antifertility effect of the lower dose of antibody when replacement began on day 2, 3 or 4, though the number of implantation sites was reduced when treatment started on day 3 or 4. By day 5 only one of six treated animals remained pregnant, showing that antibody action was reversible only up to day 4. At the higher dose of antibody, exogenous nidatory oestrogen was also required. Pseudopregnant mice were injected 32 h p.c. with this antibody (5·7 nmol IgG) or 0·9% NaCl (controls). At 16.00 h on day 4 p.c., oil was injected into the lumen of one uterine horn and the magnitude of the decidual cell reaction was assessed 72 h later. Injected horns of antibody-treated females did not respond to intraluminal oil, whereas those of control mice increased fivefold in weight. Steroid treatment after the induction stimulus did not promote decidual growth, indicating that passive immunization reduced endometrial sensitivity. The results show that in the event that antibody fails to arrest the development of all embryos, the absence of endometrial sensitization will preclude the initiation of implantation, unless progesterone is given within 48 h of antibody treatment. J. Endocr. (1985) 104, 153–158

PSXII-18 Immunoglobulin G content and composition of colostrum in different cattle breeds

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 419-420
Author(s):  
Evelyne C Kessler ◽  
Rupert M Bruckmaier ◽  
Josef J Gross
Keyword(s):  
Dairy Cows ◽  
Immunoglobulin G ◽  
Fixed Effects ◽  
Passive Immunization ◽  
Mature Milk ◽  
Dual Purpose ◽  
Cattle Breeds ◽  
Holstein Friesian ◽  
Parity Number ◽  
And Performance

Abstract Immunoglobulins (Ig) are essential bioactive components in colostrum that enable passive immunization of newborn calves. Compared to mature milk, concentrations of fat and protein are greater in colostrum, and represent a vital source of energy and nutrients. Colostral IgG was shown to vary between individual dairy cows, but comparative data on different breeds and performance levels are scarce. The objective of the present field study was to investigate the contents of total IgG, fat, protein, and lactose in colostrum in different Swiss and German dairy and dual-purpose breeds. We collected colostrum samples of 458 cows of 13 different breeds (dairy breeds: Brown Swiss, Swiss and German Holstein Friesian, and New Zealand Holstein; dual-purpose breeds: German Fleckvieh, Holstein Friesian × Montbéliarde, Montbéliarde, Murnau-Werdenfels, Original Braunvieh, Pinzgauer, Rhetic Gray, and Simmental; beef type crossbred Charolais × Holstein Friesian). Colostrum samples were obtained between 5 and 900 min after calving and analyzed for total IgG, fat protein, and lactose contents. Statistical analysis was carried out using a GLM model with breed, parity number, and time of colostrum collection as fixed effects. Immunoglobulin G concentrations varied between 12.7 and 204.0 mg/mL. No effect of breeding purpose (i.e., dairy or dual-purpose), nor of previous lactation yield on IgG content was observed. However, milking of cows for the first time later than 12 h after parturition resulted in lower colostrum IgG concentrations compared to colostrum harvest within 9 h after calving (P &lt; 0.05). Multiparous cows had a higher colostral IgG concentration than primiparous cows (P &lt; 0.0001). Overall, concentrations of IgG and other constituents in colostrum varied widely in the different cattle breeds. High-yielding dairy cows did not have poorer colostrum quality compared to lower yielding animals or beef and dual-purpose breeds, which suggests an individually different transfer of circulating IgG into colostrum.

Studies on the supply of immunoglobulin G to newborn camel calves (Camelus dromedarius)

2001 ◽  
Vol 68 (1) ◽  
pp. 1-7 ◽  
Author(s):  
ROLF KAMBER ◽  
ZAKARIA FARAH ◽  
PETER RUSCH ◽  
MICHAEL HASSIG
Keyword(s):  
Immunoglobulin G ◽  
Management Practice ◽  
Passive Immunization ◽  
Critical Value ◽  
Early Mortality ◽  
Life Evaluation ◽  
Newborn Calves ◽  
Calf Mortality ◽  
Igg Level ◽  
Poor Management

A major problem in camel productivity is the high mortality rate of camel calves in the first 3 months. The causes for mortality are mainly poor management practice and infectious diseases. The purpose of this research, carried out on a ranch in Kenya, was to determine the immunoglobulin G (IgG) concentration in camel colostrum as well as the extent of the calves' passive immunization by maternal antibodies. IgG concentration in colostrum and in the serum of the calf were measured during the first 3 d of life. Evaluation was carried out by comparing the respective values with those for horses and cattle. The average IgG concentration in the camel colostrum was higher than that found in literature for horses and cattle. IgG concentration in the serum of the camel calves reached its maximum 24 h after birth. In 39% of the examined calves, this maximum concentration was below 4 g/l, which is considered to be the critical value in horses and cattle. 61% of the calves achieved an IgG concentration of over 4 g/l. Since there is no correlation between IgG level in colostrum and early mortality, the results indicate that low colostrum intake during the first 24 h of life and not low IgG concentration in colostrum is presumably one of the main causes of early calf mortality. Therefore, it was recommended that the care of the newborn calves by herdsmen should be improved.

Use of Protein a Conjugated to Peroxidase to Localize Immunoglobulin G in SSPE Ferret Brain

1982 ◽  
Vol 40 ◽  
pp. 350-351
Author(s):  
Hannah R. Brown ◽  
Anthony F. Nostro ◽  
Halldor Thormar
Keyword(s):  
Immunoglobulin G ◽  
Human Disease ◽  
Measles Virus ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein A ◽  
Inflammatory Lesions ◽  
Sspe Virus ◽  
Specific Immunoglobulin ◽  
Antibody Titers ◽  
The Brain

Subacute sclerosing panencephalitis (SSPE) is a slowly progressing disease of the CNS in children which is caused by measles virus. Ferrets immunized with measles virus prior to inoculation with the cell associated, syncytiogenic D.R. strain of SSPE virus exhibit characteristics very similar to the human disease. Measles virus nucleocapsids are present, high measles antibody titers are found in the sera and inflammatory lesions are prominent in the brains. Measles virus specific immunoglobulin G (IgG) is present in the brain,and IgG/ albumin ratios indicate that the antibodies are synthesized within the CNS.

Binucleate Spermiogenesis in the Mouse

1972 ◽  
Vol 30 ◽  
pp. 112-113
Author(s):  
John J. Wolosewick
Keyword(s):  
Electron Microscopy ◽  
Phosphate Buffer ◽  
Seminiferous Epithelium ◽  
Mouse Testis ◽  
Germinal Epithelium ◽  
Intercellular Bridges ◽  
Adult Mice ◽  
Cervical Dislocation ◽  
Human And Mouse

Classically, the male germinal epithelium is depicted as synchronously developing uninucleate spermatids conjoined by intercellular bridges. Recently, binucleate and multinucleate spermatids from human and mouse testis have been reported. The present paper describes certain developmental events in one type of binucleate spermatid in the seminiferous epithelium of the mouse.Testes of adult mice (ABP Jax) were removed from the animals after cervical dislocation and placed into 2.5% glutaraldehyde/Millonig's phosphate buffer (pH 7.2). Testicular capsules were gently split and separated, exposing the tubules. After 15 minutes the tissue was carefully cut into cubes (approx. 1mm), fixed for an additional 45 minutes and processed for electron microscopy.

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