Toxicity of Intravenous Catheters Tested in a Human Bone Marrow Culture System

2009 ◽  
Vol 202 (1-6) ◽  
pp. 103-106 ◽  
Author(s):  
B. Thing Mortensen ◽  
Lis E. Jensen ◽  
Seren Knudtzon ◽  
Nis I. Nissen
Keyword(s):  
Bone Marrow ◽  
Culture System ◽  
Bone Marrow Culture ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Intravenous Catheters

A Stro-1+ human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serum-free culture system

2006 ◽  
Vol 34 (10) ◽  
pp. 1353-1359 ◽  
Author(s):  
Raquel Gonçalves ◽  
Cláudia Lobato da Silva ◽  
Joaquim M.S. Cabral ◽  
Esmail D. Zanjani ◽  
Graça Almeida-Porada
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Culture System ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Feeder Layer ◽  
Hematopoietic Stem ◽  
Serum Free

The development of a liquid culture system for the growth of human bone marrow

1980 ◽  
Vol 4 (5) ◽  
pp. 449-461 ◽  
Author(s):  
I.R.G. Toogood ◽  
T.M. Dexter ◽  
T.D. Allen ◽  
T. Suda ◽  
L.G. Lajtha
Keyword(s):  
Bone Marrow ◽  
Culture System ◽  
Liquid Culture ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Liquid Culture System

scholarly journals Long-term human bone marrow cultures

Blood ◽  
1980 ◽  
Vol 56 (1) ◽  
pp. 118-124
Author(s):  
WG Hocking ◽  
DW Golde
Keyword(s):  
Bone Marrow ◽  
Culture System ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Murine Bone Marrow ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures

Pronlonged in vitro growth of murine bone marrow has been achieved using a flask culture system. We report the adaption of the technique to the growth of human bone marrow. In this system, CFU-GM and CFU-E are maintained for 4–9 wk. Morphologically recognizable granulopoiesis occurs for 4–6 wk and erythropoiesis for 1.5–2.5 wk. Functional T lymphocytes are maintained for at least 5 wk. The adherent population is composed of macrophages, fibroblast-like cells, and flat pavement- like cells. Fat-containing cells are not prominant. This culture system provides a means to study human hematopoietic cell proliferation and differentiation in vitro, as well as to examine interactions between stromal cells and hematopoietic and lymphoid progenitors.

scholarly journals Response kinetics of radiation-induced micronucleated reticulocytes in human bone marrow culture

2011 ◽  
Vol 718 (1-2) ◽  
pp. 38-43 ◽  
Author(s):  
Hongliang Sun ◽  
Ying Tsai ◽  
Irena Nowak ◽  
Stephen D. Dertinger ◽  
J.H. David Wu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Culture ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Radiation Induced ◽  
Kinetics Of

HUMAN SERUM FACTOR INDUCING GIANT FAT CELL FORMATION IN HUMAN BONE MARROW CULTURE: NORMAL AND LEUKEMIC

1982 ◽  
pp. 379-395
Author(s):  
Inna A. Svet-Moldavskaya ◽  
George J. Svet-Moldavsky ◽  
Svetlana N. Zinzar ◽  
Carol Vergara ◽  
James F. Holland ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Human Serum ◽  
Cell Formation ◽  
Bone Marrow Culture ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Serum Factor ◽  
Fat Cell

scholarly journals Vitamin K stimulates osteoblastogenesis and inhibits osteoclastogenesis in human bone marrow cell culture

2003 ◽  
Vol 176 (3) ◽  
pp. 339-348 ◽  
Author(s):  
Y Koshihara ◽  
K Hoshi ◽  
R Okawara ◽  
H Ishibashi ◽  
S Yamamoto
Keyword(s):  
Bone Marrow ◽  
Vitamin K ◽  
Stromal Cells ◽  
Bone Marrow Cells ◽  
Osteoclast Formation ◽  
Bone Marrow Culture ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Osteogenic Potential ◽  
Marrow Cells

Accumulating evidence indicates that menaquinone-4 (MK-4), a vitamin K(2) with four isoprene units, inhibits osteoclastogenesis in murine bone marrow culture, but the reason for this inhibition is not yet clear, especially in human bone marrow culture. To clarify the inhibitory mechanism, we investigated the differentiation of colony-forming-unit fibroblasts (CFU-Fs) and osteoclasts in human bone marrow culture, to learn whether the enhancement of the differentiation of CFU-Fs from progenitor cells might relate to inhibition of osteoclast formation. Human bone marrow cells were grown in alpha-minimal essential medium with horse serum in the presence of MK-4 until adherent cells formed colonies (CFU-Fs). Colonies that stained positive for alkaline phosphatase activity (CFU-F/ALP(+)) were considered to have osteogenic potential. MK-4 stimulated the number of CFU-F/ALP(+) colonies in the presence or absence of dexamethasone. The stimulation was also seen in vitamin K(1) treatment. These cells had the ability to mineralize in the presence of alpha-glycerophosphate. In contrast, both MK-4 and vitamin K(1) inhibited 1,25 dihydroxyvitamin D(3)-induced osteoclast formation and increased stromal cell formation in human bone marrow culture. These stromal cells expressed ALP and Cbfa1. Moreover, both types of vitamin K treatment decreased the expression of receptor activator of nuclear factor kappaB ligand/osteoclast differentiation factor (RANKL/ODF) and enhanced the expression of osteoprotegerin/osteoclast inhibitory factor (OPG/OCIF) in the stromal cells. The effective concentrations were 1.0 microM and 10 microM for the expression of RANKL/ODF and OPG/OCIF respectively. Vitamin K might stimulate osteoblastogenesis in bone marrow cells, regulating osteoclastogenesis through the expression of RANKL/ODF more than through that of OPG/OCIF.

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