Mixed megakaryocytic-granulocytic differentiation during diffusion chamber culture of peripheral blast cells from the blast crisis of chronic myelocytic leukemia

1985 ◽  
Vol 9 (8) ◽  
pp. 1031-1041 ◽  
Author(s):  
Arnold Ganser ◽  
Felix Carbonell ◽  
Peter M. Lansdorp ◽  
Dieter Hoelzer
Keyword(s):  
Blast Crisis ◽  
Diffusion Chamber ◽  
Chronic Myelocytic Leukemia ◽  
Granulocytic Differentiation ◽  
Myelocytic Leukemia ◽  
Blast Cells ◽  
Diffusion Chamber Culture

scholarly journals Ph1-positive childhood leukemias: spectrum of lymphoid-myeloid expressions

Blood ◽  
1977 ◽  
Vol 49 (4) ◽  
pp. 549-558 ◽  
Author(s):  
EN Forman ◽  
T Padre-Mendoza ◽  
PS Smith ◽  
BE Barker ◽  
P Farnes
Keyword(s):  
Lymphoblastic Leukemia ◽  
Blast Crisis ◽  
Leukemic Cells ◽  
Chronic Myelocytic Leukemia ◽  
Therapeutic Approaches ◽  
Second Child ◽  
Myelocytic Leukemia ◽  
Surface Receptor ◽  
Blast Cells ◽  
Cell Series

Abstract Two children presented with Ph1-positive leukemia, confirmed by Giemsa banding as 22q-. One child showed an initial presentation characteristic of acute lymphoblastic leukemia, followed by development of chronic myelocytic leukemia 2 yr later. A second child presented in blast crisis. Both patients showed blast cells possessing both lymphoid and myeloid characteristics, as demonstrated by histochemical, biochemical, or surface receptor properties of each cell series. The evidence provided supports the assumption of mixed lymphoid-myeloid properties of blast cells in chronic myelocytic leukemia in children. Detailed study of the leukemic cells may aid in the understanding of complex stem cell relationships and suggest more effective therapeutic approaches.

scholarly journals Ph1-positive childhood leukemias: spectrum of lymphoid-myeloid expressions

Blood ◽  
1977 ◽  
Vol 49 (4) ◽  
pp. 549-558 ◽  
Author(s):  
EN Forman ◽  
T Padre-Mendoza ◽  
PS Smith ◽  
BE Barker ◽  
P Farnes
Keyword(s):  
Lymphoblastic Leukemia ◽  
Blast Crisis ◽  
Leukemic Cells ◽  
Chronic Myelocytic Leukemia ◽  
Therapeutic Approaches ◽  
Second Child ◽  
Myelocytic Leukemia ◽  
Surface Receptor ◽  
Blast Cells ◽  
Cell Series

Two children presented with Ph1-positive leukemia, confirmed by Giemsa banding as 22q-. One child showed an initial presentation characteristic of acute lymphoblastic leukemia, followed by development of chronic myelocytic leukemia 2 yr later. A second child presented in blast crisis. Both patients showed blast cells possessing both lymphoid and myeloid characteristics, as demonstrated by histochemical, biochemical, or surface receptor properties of each cell series. The evidence provided supports the assumption of mixed lymphoid-myeloid properties of blast cells in chronic myelocytic leukemia in children. Detailed study of the leukemic cells may aid in the understanding of complex stem cell relationships and suggest more effective therapeutic approaches.

scholarly journals Persistence of chronic myelocytic leukemia despite deletion of rearranged bcr/c-abl sequences in blast crisis.

1986 ◽  
Vol 164 (5) ◽  
pp. 1389-1396 ◽  
Author(s):  
C R Bartram ◽  
J W Janssen ◽  
R Becher ◽  
A de Klein ◽  
G Grosveld
Keyword(s):  
In Situ Hybridization ◽  
T Cell ◽  
Southern Blot ◽  
Blast Crisis ◽  
Cell Lineage ◽  
Chronic Myelocytic Leukemia ◽  
Northern Blots ◽  
Myelocytic Leukemia ◽  
Ph Chromosome

We report on a Ph+ chronic myelocytic leukemia (CML) case, cytogenetically characterized by the occurrence of a second Philadelphia (Ph) chromosome in lymphoid blast crisis of T cell lineage. In situ hybridization analyses showed a deletion of translocated c-abl sequences, present on the Ph during chronic state, from both Ph in acute state. Moreover, Southern blot analyses of blastic cells exhibited a rearrangement within bcr, but a deletion of 5' bcr sequences, and Northern blots failed to detect the hybrid 8.5 kb bcr/c-abl transcript usually observed in Ph+ CML.

scholarly journals Promyelocytic blast crisis of chronic myelocytic leukemia with both t(9;22) and t(15;17) in M3 cells

Cancer ◽  
1984 ◽  
Vol 54 (11) ◽  
pp. 2409-2413 ◽  
Author(s):  
Sylvie Castaigne ◽  
Roland Berger ◽  
Veronique Jolly ◽  
Marie Therese Daniel ◽  
Alain Bernheim ◽  
...  
Keyword(s):  
Blast Crisis ◽  
Chronic Myelocytic Leukemia ◽  
Myelocytic Leukemia

scholarly journals Emergence of a cell line with extreme hypodiploidy in blast crisis of chronic myelocytic leukemia

Blood ◽  
1979 ◽  
Vol 53 (4) ◽  
pp. 707-711 ◽  
Author(s):  
RM Como ◽  
PR Graze
Keyword(s):  
Cell Line ◽  
Blast Crisis ◽  
Chronic Myelocytic Leukemia ◽  
Banding Patterns ◽  
Myelocytic Leukemia ◽  
Modal Chromosome Number ◽  
Response To Chemotherapy ◽  
Cytogenetic Studies ◽  
A Cell ◽  
Complex Chromosome

Abstract Cytogenetic studies in a patient with chronic myelocytic leukemia (CML) demonstrated the emergence of an extremely hypodiploid cell line at the time of blast crisis, a modal chromosome number of 35, with the modal karyotype 35,XY, -3, -4, -5, -7, -9, -11, -12, -13, -15, -16, -17, -19, -20, -22, + t(9;22) (q34;q11, + Mar1, + Mar2, + Mar3. Giemsa-banding confirmed complex chromosome rearrangements and demonstrated distinct banding patterns for the marker chromosomes. Cytologic characteristics of the leukemia blasts were predominantly myeloid. There was no important clinical response to chemotherapy, including vincristine and prednisone, or to radiotherapy.

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