An enzyme-linked immunosorbent assay for myelin basic protein-specific protein methylase I

A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for the rapid detection of Staphylococcus aureus in foods. The assay was based on the detection of protein A which is a specific protein secreted by S. aureus. Following a 24-h incubation in a staphylococcal selective broth containing mannitol as the carbon source, the culture supematant was added to the microtiter plate coated with anti-protein A immunoglobulin G (IgG). After incubation, peroxidase-labeled anti-protein A IgG was used to produce the signal of antigen-antibody reaction. The sensitivity of the assay for protein A was 0.1 ng/ml. For 37 strains of S. aureus studied, all produced protein A, and the amount (13-1,100 ng/ml) of protein A secreted by different strains varied to a large degree. For another 57 strains (including 19 Staphylococcus spp.) of bacteria tested, two strains (5. capilis subsp. capitis CCRC 12161 and S. lentus CCRC 12926) produced very low amounts of protein A (0.6-1 ng/ml) after 24-h incubation. Staphylococcus aureus was detected by the ELISA in all of six samples of precooked foods naturally contaminated with the bacterium. Twenty-two processed foods artificially inoculated with S. aureus at levels of < 2 CFU/g and 10 to 20 CFU/g, respectively, were all positive by the ELISA. As compared to the conventional culture methods which take 5 to 6 days to complete, the ELISA can detect low numbers of S. aureus in processed foods with a total analytical time of only 28 h.

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Changes in myelin basic protein and demyelination in the rat brain within 3 months of single 2-, 10-, or 30-Gy whole-brain radiation treatments

Journal of Neurosurgery ◽

10.3171/jns/2008/109/11/0881 ◽

2008 ◽

Vol 109 (5) ◽

pp. 881-888 ◽

Cited By ~ 17

Author(s):

Ye Tian ◽

Zhige Shi ◽

Shu Yang ◽

Yingzhu Chen ◽

Shiyao Bao

Keyword(s):

Myelin Basic Protein ◽

Brain Tissue ◽

Basic Protein ◽

Enzyme Linked Immunosorbent Assay ◽

Whole Brain ◽

Whole Brain Radiation ◽

Injured Brain ◽

Brain Radiation ◽

The Brain ◽

Myelin Staining

Object The aim of this study was to determine the relation between changes in myelin basic protein (MBP) levels during the acute and subacute phases of central nervous system injury following whole-brain radiation and delayed demyelination in the radiation-injured brain tissue. Methods Adult Sprague–Dawley rats were treated with single fractions of 2, 10, or 30 Gy of whole-brain radiation. The authors measured MBP gene expression and protein levels in the brain tissue by using reverse transcription–polymerase chain reaction and enzyme-linked immunosorbent assay at 1 week and 1–3 months following irradiation to monitor myelin changes in the brain. Demyelination was determined with Luxol fast blue myelin staining and routine histopathological and electron microscopy examination of injured brain tissue. The changes in MBP levels in the different animal groups at specific time points were correlated with demyelination in corresponding dose groups. Results At 1 month after applying the 10 and 30 Gy of radiation, MBP mRNA expression showed a transient but significant decrease, followed by recovery to baseline levels at 3 months after treatment. The MBP levels were decreased by only 70–75% at 1 month after 10 and 30 Gy of radiation. At 2–3 months after applying the higher dose of 30 Gy, however, the MBP levels continued to decline, and typical demyelination changes were observed with myelin staining and ultrastructural examination. Conclusions The authors' results suggest that the early radiation-induced MBP changes between 1 and 3 months after single treatments of 10 and 30 Gy of radiation to the whole brain are indicative of permanent injury shown as demyelination of irradiated brain tissue.

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Identification of unacceptable background caused by non-specific protein adsorption to the plastic surface of 96-well immunoassay plates using a standardized enzyme-linked immunosorbent assay procedure

Journal of Immunological Methods ◽

10.1016/s0022-1759(99)00051-4 ◽

1999 ◽

Vol 226 (1-2) ◽

pp. 85-92 ◽

Cited By ~ 40

Author(s):

Dierk E Rebeski ◽

Eva M Winger ◽

Yeun-Kyung Shin ◽

Mamadou Lelenta ◽

Mark M Robinson ◽

...

Keyword(s):

Protein Adsorption ◽

Immunosorbent Assay ◽

Specific Protein ◽

Enzyme Linked Immunosorbent Assay ◽

Assay Procedure ◽

Plastic Surface

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Studies on myelin basic protein-specific protein methylase I in various dysmyelinating mutant mice

Biochemical and Biophysical Research Communications ◽

10.1016/0006-291x(84)90254-7 ◽

1984 ◽

Vol 123 (2) ◽

pp. 468-474 ◽

Cited By ~ 21

Author(s):

Sangduk Kim ◽

Martin Tuck ◽

Myunghee Kim ◽

Anthony T. Campagnoni ◽

Woon Ki Paik

Keyword(s):

Myelin Basic Protein ◽

Basic Protein ◽

Specific Protein ◽

Mutant Mice

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Myelin basic protein and neurofilament H in postmortem cerebrospinal fluid as surrogate markers of fatal traumatic brain injury

International Journal of Legal Medicine ◽

10.1007/s00414-021-02606-y ◽

2021 ◽

Author(s):

Simone Bohnert ◽

Christoph Wirth ◽

Werner Schmitz ◽

Stefanie Trella ◽

Camelia-Maria Monoranu ◽

...

Keyword(s):

Traumatic Brain Injury ◽

Cerebrospinal Fluid ◽

Brain Injury ◽

Myelin Basic Protein ◽

Immunohistochemical Staining ◽

Basic Protein ◽

Staining Intensity ◽

Enzyme Linked Immunosorbent Assay ◽

Staining Reaction ◽

Surrogate Markers

AbstractThe aim of this study was to investigate if the biomarkers myelin basic protein (MBP) and neurofilament-H (NF-H) yielded informative value in forensic diagnostics when examining cadaveric cerebrospinal fluid (CSF) biochemically via an enzyme-linked immunosorbent assay (ELISA) and comparing the corresponding brain tissue in fatal traumatic brain injury (TBI) autopsy cases by immunocytochemistry versus immunohistochemistry. In 21 trauma and 19 control cases, CSF was collected semi-sterile after suboccipital puncture and brain specimens after preparation. The CSF MBP (p = 0.006) and NF-H (p = 0.0002) levels after TBI were significantly higher than those in cardiovascular controls. Immunohistochemical staining against MBP and against NF-H was performed on cortical and subcortical samples from also biochemically investigated cases (5 TBI cases/5 controls). Compared to the controls, the TBI cases showed a visually reduced staining reaction against MBP or repeatedly ruptured neurofilaments against NF-H. Immunocytochemical tests showed MBP-positive phagocytizing macrophages in CSF with a survival time of > 24 h. In addition, numerous TMEM119-positive microglia could be detected with different degrees of staining intensity in the CSF of trauma cases. As a result, we were able to document that elevated levels of MBP and NF-H in the CSF should be considered as useful neuroinjury biomarkers of traumatic brain injury.

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