Serum levels of major basic protein in patients with or without eosinophilia: measurement by enzyme-linked immunosorbent assay

1994 ◽  
Vol 86 (3) ◽  
pp. 490-495 ◽  
Author(s):  
Harumi Yamamoto ◽  
Haruhiko Ninomiya ◽  
Kentaro Yoshimatsu ◽  
Yasuo Uchiyama ◽  
Masanao Shibasaki ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Basic Protein ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Major Basic Protein

scholarly journals HUBUNGAN KADAR VITAMIN D SERUM DENGAN KUALITAS HIDUP PADA PASIEN EPILEPSI

2018 ◽  
Vol 35 (4) ◽  
Author(s):  
Edith Fitriyana Girsang ◽  
Aris Catur Bintoro ◽  
Dwi Pudjonarko
Keyword(s):  
Quality Of Life ◽  
Vitamin D ◽  
Immunosorbent Assay ◽  
Serum Vitamin ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Epilepsy Patient ◽  
Vitamin D Levels ◽  
Spearman Test

  THE CORRELATION BETWEEN VITAMIN D SERUM LEVELS WITH QUALITY OF LIFE IN EPILEPSY PATIENTABSTRACTIntroduction: Epilepsy affects overall health status and decreases the life quality of epilepsy patient. Seizure frequency, seizure type, daily activity disorder, depression and anxiety also affect the quality of life epilepsy patient. Vitamin D is considered as a neurosteroid modulator of nerve excitability and seizure susceptibility. Studies of vitamin D direct role in epilepsy are limited. Nevertheless, some studies show the role of vitamin D as an anticonvulsant that reduces the incidence of seizures.Aim: To determine the correlation between vitamin D serum levels with quality of life in epilepsy patient.Method: A cross sectional observational study on people with epilepsy in neurology clinic at Dr. Soeselo Hospital, Slawi, Dr Kariadi Hospital, and Tugurejo Hospital, Semarang in July 2017-January 2018. The quality of life was assessed with Quality of Life in Epilepsy 31 (QOLIE-31). Vitamin D serum levels measured using enzyme-linked immunosorbent assay (ELISA) method. Data were analyzed using Spearman test. Results were considered significant if p<0.05.Results: There was no correlation between vitamin D serum levels with quality of life in epilepsy. There was significant correlation between anxiety with quality of life and there were difference between age group with quality of life. Discussion: There was no correlation between serum vitamin D levels and  quality of life in epilepsy patient.Keywords: Epilepsy,quality of life epilepsy, vitamin DABSTRAKPendahuluan: Epilepsi mempengaruhi status kesehatan secara keseluruhan dan menurunkan kualitas hidup pasien epilepsi. Frekuensi bangkitan, tipe bangkitan, gangguan aktivitas harian, depresi dan ansietas, juga memengaruhi kualitas hidup pasien epilepsi. Vitamin D dianggap neurosteroid, sebagai modulator eksitabilitas saraf dan kerentanan bangkitan. Bukti langsung untuk peran vitamin D dalam epilepsi terbatas. Namun beberapa penelitian menunjukkan peran vitamin D sebagai antikonvulsan yang mengurangi kejadian bangkitan.Tujuan: Mengetahui hubungan kadar vitamin D serum dengan kualitas hidup pada pasien epilepsi.Metode: Penelitian potong lintang terhadap pasien epilepsi yang berobat ke Poliklinik Saraf RSUD Dr. Soeselo, Slawi, RSUP Dr. Kariadi, Semarang, dan RS Tugurejo, Semarang pada bulan Juli 2017-Januari 2018. Kualitas hidup dinilai menggunakan kuesioner Quality of Life in Epilepsy 31 (QOLIE-31), pengukuran kadar vitamin D serum menggunakan metode enzyme-linked immunosorbent assay (ELISA). Data dianalisis dengan uji Spearman, hasil dianggap bermakna jika p<0,05.Hasil: Tidak didapatkan hubungan antara kadar vitamin D serum dengan kualitas hidup serta domainnya pada pasien epilepsi. Didapatkan hubungan yang bermakna antara ansietas dengan kualitas hidup dan antara kelompok usia dengan kualitas hidup.Diskusi: Tidak terdapat hubungan bermakna antara kadar vitamin D serum dengan kualitas hidup pada pasien epilepsi.Kata kunci: Epilepsi, kualitas hidup, vitamin D

scholarly journals Analysis ofLeishmaniamimetic neoglycoproteins for the cutaneous leishmaniasis diagnosis

Parasitology ◽  
2018 ◽  
Vol 145 (14) ◽  
pp. 1938-1948 ◽  
Author(s):  
Lígia Moraes Barizon de Souza ◽  
Vanete Thomaz Soccol ◽  
Ricardo Rasmussen Petterle ◽  
Michelle D. Bates ◽  
Paul A. Bates
Keyword(s):  
Cell Surface ◽  
Cutaneous Leishmaniasis ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Individuals ◽  
Cell Surfaces ◽  
Cell Markers ◽  
Antibody Titres

AbstractOligosaccharides are broadly present onLeishmaniacell surfaces. They can be useful for the leishmaniases diagnosis and also helpful in identifying new cell markers for the disease. The disaccharide Galα1-3Galβis the immunodominant saccharide inLeishmaniacell surface and is the unique non-reducing terminal glycosphingolipids structure recognized by anti-α-Gal. This study describes an enzyme-linked immunosorbent assay (ELISA) used to measure serum levels of anti-α-galactosyl (α-Gal) antibodies in patients with cutaneous leishmaniasis (CL). Optimal ELISA conditions were established and two neoglycoproteins (NGP) containing the Galα1-3Gal terminal fraction (Galα1-3Galβ1-4GlcNAc-HAS and Galα1-3Gal-HAS) and one Galα1-3Gal NGP analogue (Galα1-3Galβ1-3GlcNAc-HAS) were used as antigens. Means of anti-α-Gal antibody titres of CL patients were significantly higher (P< 0.05) than the healthy individuals for all NGPs tested. Sensitivity and specificity of all NGPs ranged from 62.2 to 78.4% and 58.3 to 96.7%, respectively. In conclusion, the NGPs can be used for CL diagnosis.

scholarly journals Elevated serum levels of the eosinophil granule major basic protein in patients with eosinophilia.

1981 ◽  
Vol 67 (3) ◽  
pp. 651-661 ◽  
Author(s):  
D L Wassom ◽  
D A Loegering ◽  
G O Solley ◽  
S B Moore ◽  
R T Schooley ◽  
...  
Keyword(s):  
Basic Protein ◽  
Elevated Serum ◽  
Serum Levels ◽  
Major Basic Protein ◽  
Eosinophil Granule

scholarly journals Elevated serum levels in human pregnancy of a molecule immunochemically similar to eosinophil granule major basic protein.

1983 ◽  
Vol 158 (4) ◽  
pp. 1211-1226 ◽  
Author(s):  
D E Maddox ◽  
J H Butterfield ◽  
S J Ackerman ◽  
C B Coulam ◽  
G J Gleich
Keyword(s):  
Guinea Pig ◽  
Pregnant Women ◽  
Dose Response ◽  
Basic Protein ◽  
Serum Levels ◽  
Major Basic Protein ◽  
Response Curves ◽  
Double Antibody ◽  
Eosinophil Granule ◽  
In Pregnancy

We have shown that serum levels of a molecule immunochemically similar to eosinophil granule major basic protein (MBP) are elevated in pregnant women throughout gestation. MBP levels increase during gestation and plateau at approximately 7,500 ng/ml by the 20th wk (greater than 10-fold above normal). Levels return to normal after delivery, with a T1/2 of 13.7 d. The MBP in pregnancy serum is remarkably similar to the eosinophil granule MBP in that: (a) pregnancy MBP fully inhibits the binding of radiolabeled MBP standard in a double antibody radioimmunoassay; (b) this inhibition reaction is specific for human MBP because pregnancy serum produces no inhibition of the binding of radiolabeled guinea pig MBP in the guinea pig MBP radioimmunoassay; (c) in a two-site immunoradiometric assay for MBP, slopes of dose-response curves for pregnancy serum, purified MBP, and serum from a patient with hypereosinophilic syndrome are identical, and maximal binding is comparable; (d) reduction and alkylation of pregnancy sera increases measured MBP 100-fold, as previously shown for eosinophil granule MBP in serum; and (e) the MBP in pregnancy serum demonstrates the same pattern of heat lability as has been previously reported for MBP. Four observations have raised the possibility that the eosinophil is not the source of the MBP in pregnancy serum: (a) no correlation between serum MBP level and peripheral blood eosinophil count exists in pregnant women, in contrast to previous studies of patients with eosinophilia; (b) levels of three other eosinophil-associated proteins are normal or low in pregnancy sera, whereas the serum levels of these proteins are elevated in patients with eosinophilia; (c) the slopes of dose-response curves for pregnancy sera and MBP standards differ in the double antibody radioimmunoassay; and (d) the molecule in pregnancy serum elutes from Sephadex G-50 columns at the void volume, while eosinophil granule MBP and the MBP in serum of patients with eosinophilia elute at a volume consistent with the previously established molecular weight of 9,300. These findings suggest that the MBP in pregnancy serum is derived from a source other than the eosinophil.

scholarly journals Serum interleukin-30 level in patients with psoriasis and its correlation with psoriasis severity: a case-control study

2021 ◽  
Vol 49 (4) ◽  
pp. 030006052110040
Author(s):  
Nergez Sabah Omar ◽  
Xinxin Long ◽  
Jiayi Xian ◽  
Henok Kessete Afewerky ◽  
Saeed Ghulam Hussain ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Immunosorbent Assay ◽  
Case Control Study ◽  
Severity Index ◽  
Serum Levels ◽  
Case Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Psoriasis Severity ◽  
Control Study

Objectives To examine the serum levels of interleukin (IL)-30 in patients with psoriasis and evaluate the correlations with the Psoriasis Area and Severity Index (PASI). Methods Serum was collected from 26 patients with psoriasis and 26 healthy controls in a case–control setting, and the level of IL-30 was determined using an enzyme-linked immunosorbent assay. Statistical analysis of the IL-30 levels among groups and further correlation analyses of IL-30 levels with PASI scores were performed. Results A significant increase in the level of IL-30 in patients with psoriasis compared with healthy controls was observed. In addition, a positive correlation between the IL-30 concentration and PASI scores was found in patients with psoriasis. Conclusion IL-30 is presumably involved in the proliferation of epidermal cells during the development of psoriasis. Further studies with a larger number of participants are required to comprehensively elucidate the biological roles of IL-30 in the pathogenesis of psoriasis.

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