An investigation of allogeneic pregnancy in multiparous mice subjected to in vivo depletion of CD8 (Ly2)-positive lymphocytes by monoclonal antibody treatment

1988 ◽  
Vol 14 (3) ◽  
pp. 235-245 ◽  
Author(s):  
Pirkko Sulila ◽  
Rikard Holmdahl ◽  
Inga Hansson ◽  
Folke Bernadotte ◽  
Anita Mattsson ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Treatment ◽  
Monoclonal Antibody Treatment

scholarly journals The CD154/CD40 Interaction Required for Retrovirus-Induced Murine Immunodeficiency Syndrome Is Not Mediated by Upregulation of the CD80/CD86 Costimulatory Molecules

2002 ◽  
Vol 76 (24) ◽  
pp. 13106-13110 ◽  
Author(s):  
Kathy A. Green ◽  
W. James Cook ◽  
Arlene H. Sharpe ◽  
William R. Green
Keyword(s):  
Monoclonal Antibody ◽  
B Cells ◽  
Molecular Interactions ◽  
T And B Cells ◽  
Antibody Treatment ◽  
Monoclonal Antibody Treatment ◽  
Murine Aids ◽  
Cd40 Expression ◽  
Immunodeficiency Syndrome

ABSTRACT C57BL/6 (B6) mice infected with LP-BM5 retroviruses develop disease, including an immunodeficiency similar to AIDS. This disease, murine AIDS (MAIDS), is inhibited by in vivo anti-CD154 monoclonal antibody treatment. The similar levels of insusceptibility of CD40−/− and CD154−/− B6 mice indicate that CD154/CD40 molecular interactions are required for MAIDS. CD4+ T and B cells, respectively, provide the CD154 and CD40 expression needed for MAIDS induction. Here, the required CD154/CD40 interaction is shown to be independent of CD80 and CD86 expression: CD80/CD86−/− B6 mice develop MAIDS after LP-BM5 infection.

scholarly journals A monoclonal antibody recognizing CD43 (leukosialin) initiates apoptosis of human hematopoietic progenitor cells but not stem cells

Blood ◽  
1996 ◽  
Vol 87 (4) ◽  
pp. 1272-1281 ◽  
Author(s):  
V Bazil ◽  
J Brandt ◽  
S Chen ◽  
M Roeding ◽  
K Luens ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Progenitor Cells ◽  
Lymphoid Cells ◽  
Hematopoietic Progenitor Cells ◽  
Proliferative Potential ◽  
Hematopoietic Progenitor ◽  
Antibody Treatment ◽  
Hematopoietic Stem ◽  
Monoclonal Antibody Treatment

CD43 (the major sialoglycoprotein of leukocytes) is an adhesion molecule broadly expressed on hematopoietic cells. A monoclonal antibody recognizing this molecule induces apoptosis of lineage marker- negative bone marrow hematopoietic progenitor cells (HPCs) that express CD34 at a high density (CD34hiLIN-). However, not all cells within this population undergo apoptosis on stimulation via CD43. Dividing progenitor cells are most highly affected, whereas more primitive quiescent cells survive anti-CD43 monoclonal antibody treatment. These surviving cells (1) are enriched for cobblestone area-forming cells, (2) repopulate fragments for human fetal bone implanted into C.B-17 scid/scid mice, (3) have a potential to differentiate in vivo to myeloid and lymphoid cells, and (4) have a high proliferative potential in long-term stromal cell-free liquid culture. These data indicate that cells with hematopoietic stem cell characteristics are relatively resistant to CD43-mediated apoptosis as compared with HPCs. Thus, CD43 may be specifically involved in the regulation of HPC proliferation.

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