Mutagenic effectiveness and efficiency of sodium azide versus ethyl methanesulfonate in maize: Induction of somatic mutations at the yg2 locus by treatment of seeds differing in metabolic state and cell population

1977 ◽  
Vol 46 (4) ◽  
pp. 285-295 ◽  
Author(s):  
B.V. Conger ◽  
J.V. Carabia
Keyword(s):  
Cell Population ◽  
Sodium Azide ◽  
Somatic Mutations ◽  
Ethyl Methanesulfonate ◽  
Metabolic State ◽  
Effectiveness And Efficiency

Chromium (VI) Potentiates Mutagenesis by Sodium Azide But Not Ethyl Methanesulfonate

1984 ◽  
Vol 6 (3) ◽  
pp. 311-320 ◽  
Author(s):  
James M. Lavelle ◽  
Charlotte M. Witmer
Keyword(s):  
Sodium Azide ◽  
Ethyl Methanesulfonate ◽  
Chromium Vi

scholarly journals Sodium azide enhancement of interleukin-2 production

Blood ◽  
1983 ◽  
Vol 61 (4) ◽  
pp. 815-818 ◽  
Author(s):  
SP Feldman ◽  
R Mertelsmann ◽  
S Venuta ◽  
M Andreeff ◽  
K Welte ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Cell Population ◽  
Sodium Azide ◽  
Human Lymphocytes ◽  
Interleukin 2 ◽  
Final Concentration ◽  
Cell Cycle Analysis ◽  
Normal Human ◽  
Responsive Cell

Abstract The production of large quantities of Interleukin-2 (IL-2) from normal human lymphocytes has been limited by the short production and release period, as well as its absorption by the responsive cell population. We report the utilization of sodium azide (NaN3, 0.01% final concentration) to allow continued production of IL-2 as long as 72–96 hr and thereby increase the yield significantly. Cell cycle analysis performed by flow cytometry indicates that NaN3 blocks cells at the G0- G1 transition and the G1-S transition, depending on the time of addition of NaN3 to the cultures.

scholarly journals The mutation rate in PIG-A is normal in patients with paroxysmal nocturnal hemoglobinuria (PNH)

Blood ◽  
2006 ◽  
Vol 108 (2) ◽  
pp. 734-736 ◽  
Author(s):  
David J. Araten ◽  
Lucio Luzzatto
Keyword(s):  
Cell Division ◽  
Cell Population ◽  
Mutation Rate ◽  
Genetic Instability ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Somatic Mutations ◽  
Intrinsic Rate ◽  
Large Cell ◽  
Normal Range ◽  
Hematopoietic System

Paroxysmal nocturnal hemoglobinuria (PNH) is characterized by the presence in the patient's hematopoietic system of a large cell population with a mutation in the X-linked PIG-A gene. Although this abnormal cell population is often found to be monoclonal, it is not unusual that 2 or even several PIG-A mutant clones coexist in the same patient. Therefore, it has been suggested that the PIG-A gene may be hypermutable in PNH. By a method we have recently developed for measuring the intrinsic rate of somatic mutations (μ) in humans, in which PIG-A itself is used as a sentinel gene, we have found that in 5 patients with PNH, μ ranged from 1.24 × 10–7 to 11.2 × 10–7, against a normal range of 2.4 × 10–7 to 29.6 × 10–7 mutations per cell division. We conclude that genetic instability of the PIG-A gene is not a factor in the pathogenesis of PNH.

CHEMICAL MUTAGENESIS IN TWO Cuphea SPECIES

1987 ◽  
Vol 67 (3) ◽  
pp. 909-917 ◽  
Author(s):  
T. A. CAMPBELL
Keyword(s):  
Sodium Azide ◽  
Lauric Acid ◽  
Ethyl Methanesulfonate ◽  
Chemical Mutagenesis ◽  
Mutation Rates ◽  
Distilled Water ◽  
Medium Chain Triglycerides ◽  
Medium Chain ◽  
Quantitative Characters ◽  
Short Internodes

Presoaked (24 h at 20 °C) seeds of Cuphea tolucana Peyr. and C. wrightii A. Gray were treated with mutagens at 20 °C in two experiments. Experiment 1 treatments were: distilled water (DW), 0.05 M PO4 buffer (pH 7), 0.01 M ethyl methanesulfonate (EMS) (each applied for 8 h), 0.02 M EMS (applied for 4 h), 0.04 EMS M, 0.08 M EMS, or 0.16 M EMS (each applied for 2 h). Experiment 2 treatments were: DW, 0.1 M PO4 buffer (pH 3), 0.0005 M sodium azide (SA), 0.001 M SA, or 0.002 M SA (each applied for 2 h). None of the treatments had significant effects on emergence and height of M1 plants nor were any macro-mutations noted in the M2 generations. In a third experiment, DW, 0.04 M EMS, or 0.001 M SA were applied for 2 h at 30 °C to presoaked (48 or 72 h at 30 °C) seeds of C. tolucana and C. wrightii. Compared to EMS, SA had deleterious effects on height in the M1, emergence was better for C. tolucana than for C. wrightii, and C. wrightii plants grew taller after a 72-h pre-soak than after a 48-h presoak. M2 progenies were evaluated in the field. None of the presoak-treatment combinations increased variation significantly in several quantitative characters, no macro-mutations were detected in C. wrightii, but a fertile, small-leaved, decumbent mutant with very short internodes was noted in C. tolucana. Mutation rates were greatest for the 72 h presoak-EMS combination.Key words: Cuphea tolucana, Cuphea wrightii, ethyl methanesulfonate, sodium azide, medium-chain triglycerides, lauric acid

scholarly journals Mutagenic effectiveness and efficiency of gamma rays and sodium azide in M2 generation of Cowpea [Vigna unguiculata (L.) Walp.]

2020 ◽  
Author(s):  
Aamir Raina ◽  
Samiullah Khan
Keyword(s):  
Genetic Variability ◽  
Gamma Rays ◽  
Sodium Azide ◽  
Crop Improvement ◽  
Arable Land ◽  
Grain Legume ◽  
Nutrition Security ◽  
Cowpea Varieties ◽  
Induced Mutagenesis ◽  
Effectiveness And Efficiency

AbstractLegumes play a pivotal role in combating the chronic hunger and malnutrition in the developing nations and are also ideal crops to achieve global food and nutrition security. In the era of climate change, erratic rainfalls, depleting arable land and water resource, feeding the rapidly growing population is a challenging task. Among breeding programs for crop improvement, induced mutagenesis has proven to be the viable, effective, robust and coherent tool to facilitate the process of creating varieties with improved yield. Like most other pulses, cowpea is a self pollinated, nutritious, versatile and widely adapted grain legume, but harbour a little accessible genetic variability. Lack of sufficient genetic variability and small size of flowers, traditional plant breeding methods are not enough to meet the demands of improvement of cowpea. Hence, induced mutagenesis was employed to induce significant genetic variability across a range of agro-economical traits in two cowpea varieties Gomati VU-89 and Pusa-578 from M1 to M4 generations. The success of induced mutagenesis largely depends on the selection of appropriate mutagen, its dose, effectiveness and efficiency. Hence present study was conduct to assess the effectiveness and efficiency of single and combined doses of sodium azide and gamma rays to set an appropriate protocol for induced mutagenesis experimentation in cowpea.

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