Molecular analysis of contagious pustular dermatitis virus: A simplified method for viral DNA extraction from scab material

1991 ◽  
Vol 35 (3) ◽  
pp. 265-272 ◽  
Author(s):  
C. Mazur ◽  
F.B. Rangel Filho ◽  
R. Galler
Keyword(s):  
Dna Extraction ◽  
Molecular Analysis ◽  
Viral Dna ◽  
Simplified Method ◽  
Contagious Pustular Dermatitis

Comparison of five DNA extraction methods for molecular analysis of Jerusalem artichoke (Helianthus tuberosus)

2012 ◽  
Vol 11 (1) ◽  
pp. 572-581 ◽  
Author(s):  
T. Mornkham ◽  
P.P. Wangsomnuk ◽  
P. Wangsomnuk ◽  
S. Jogloy ◽  
A. Pattanothai ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Molecular Analysis ◽  
Jerusalem Artichoke ◽  
Extraction Methods ◽  
Helianthus Tuberosus ◽  
Dna Extraction Methods

scholarly journals Detection of Badnavirus in pineapple in northeastern Brazil

2020 ◽  
Vol 5 (4) ◽  
pp. 2450-2463
Author(s):  
Edlene Maria da Silva Moraes Santos ◽  
Jaqueline Figueredo de Oliveira Costa ◽  
Mayra Machado de Medeiros Ferro ◽  
Sarah Jacqueline Cavalcanti da Silva ◽  
Iraildes Pereira Assunção ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Molecular Analysis ◽  
Distinct Species ◽  
Northeastern Brazil ◽  
Ananas Comosus ◽  
Pairwise Comparisons ◽  
Sequencing Analysis ◽  
First Report ◽  
Northeastern Region ◽  
Total Dna

ABSTRACT: Brazil is one of the main global producers of pineapple (Ananas comosus L. Merril), with emphasis in the Northeastern region of the country. Pineapple is exposed to several phytosanitary problems, including viruses. Pineapple badnaviruses are caused by two distinct species: Pineapple bacilliform CO virus (PBCoV) and Pineapple bacilliform ER virus (PBErV). The present study aimed to detect possible species of the genus Badnavirus in pineapple samples in Northeastern Brazil, via PCR and sequencing of the RT/RNaseH region. Leaf samples of pineapples were collected in the states of Alagoas, Maranhão, Paraiba and Pernambuco, and subsequently subjected to total DNA extraction and amplification via PCR. Badnavirus positive samples were selected for sequencing. Analysis of pairwise comparisons revealed that all sequences obtained in this work showed an identity greater than 80% with the sequence of the species PBCoV (EU377664), from Australia, fully corroborating with phylogenetic analyzes. These results suggest the widespread of PBCoV in Northeastern Brazil and record the first report of Badnavirus in pineapple culture in Brazil. KEYWORDS: Caulimoviridae, Ananas comosus, molecular analysis.

DNA Extraction from Bacteriophages - 96 well format v1

2021 ◽  
Author(s):  
Frej Larsen
Keyword(s):  
Dna Extraction ◽  
Viral Rna ◽  
Viral Dna

Extraction of viral DNA/RNA from culture lysate or filtered samples free from bacteria. The protocol is based on the Qiagen kit QIAamp Viral RNA Mini kit for 96 well plates and uses consumables from this kit as well as the Pierce Universal Nuclease.

scholarly journals Comparative evaluation of DNA extraction from rice’s root-associated bacterial consortium for population structure study

2021 ◽  
Vol 302 ◽  
pp. 02014
Author(s):  
Nichapat Kittiborwornkul ◽  
S.T Anne Sahithi ◽  
Jirapa Phetsom ◽  
Theerawut Phusantisampan ◽  
Wasinee Pongprayoon ◽  
...  
Keyword(s):  
Population Structure ◽  
Dna Extraction ◽  
Molecular Analysis ◽  
Bacterial Consortium ◽  
Magnetic Bead ◽  
Extraction Methods ◽  
High Yield ◽  
Spin Column ◽  
Column Adsorption ◽  
Adsorption Technology

Understanding in population structure of a plant’s root-associated microbiome is applied in good practices in agricultural activities to improve production yields and enhance plant immune responses. The molecular analysis of bacterial populations inhabited in soil faces difficulties in obtaining high yield and high purity of DNA, and different commercial DNA extraction kits have been developed for this purpose. This study focuses on the comparison of DNA extraction of six different rice root-associated bacterial consortium using three commercial kits with two key technologies, spin-column adsorption and magnetic bead adsorption. The quality and quantity of genomic DNA obtained from these extraction methods were analyzed and compared based on DNA concentration, DNA purity and efficiency to be used as a template for 16sRNA amplification. The results showed that the extraction kit with magnetic bead adsorption technology showed the highest concentration (101.32 ng/μl) compared to other DNA extraction kits (32.67 and 1.89 ng/μl). The purity values (A260/A280) were assessed by using Nano-drop spectrophotometer and resulted in purities of nucleic acids in the range of 1.4-1.7. Thus, it was concluded that the extracted DNA obtained from the extraction kit with magnetic bead adsorption technology can be valuable for molecular analysis of microbial communities present in the soil.

A molecular analysis of prokaryotic and viral DNA sequences in prostate tissue from patients with prostate cancer indicates the presence of multiple and diverse microorganisms

The Prostate ◽  
2008 ◽  
Vol 68 (3) ◽  
pp. 306-320 ◽  
Author(s):  
Karen S. Sfanos ◽  
Jurga Sauvageot ◽  
Helen L. Fedor ◽  
James D. Dick ◽  
Angelo M. De Marzo ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Molecular Analysis ◽  
Dna Sequences ◽  
Prostate Tissue ◽  
Viral Dna
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