Construction of gag, pol, and env specific riboprobes for confirmation of HIV-1 specific polymerase chain reaction products

1994 ◽  
Vol 48 (2-3) ◽  
pp. 167-175
Author(s):  
John Dolana ◽  
U. Desselberger
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction Products ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Hiv 1

Analysis of DNA restriction fragments and polymerase chain reaction products towards detection of the AIDS (HIV-1) virus in blood

1991 ◽  
Vol 559 (1-2) ◽  
pp. 267-283 ◽  
Author(s):  
Herbert E. Schwartz ◽  
Kathi Ulfelder ◽  
Franklin J. Sunzeri ◽  
Michael P. Busch ◽  
Robert G. Brownlee
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction Products ◽  
Chain Reaction ◽  
Restriction Fragments ◽  
Dna Restriction Fragments ◽  
Dna Restriction ◽  
Polymerase Chain ◽  
Hiv 1

Routine identification of four sympatric species of calanoid copepods Pseudocalanus spp. in the Atlantic Arctic using a species-specific polymerase chain reaction

2020 ◽  
Vol 48 (1) ◽  
pp. 62-72
Author(s):  
E. A. Ershova
Keyword(s):  
Polymerase Chain Reaction ◽  
Life Cycles ◽  
The Arctic ◽  
Relative Distribution ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Routine Identification ◽  
Polymerase Chain ◽  
Species Specific ◽  
Plankton Communities

Сalanoid copepods of the genus Pseudocalanus play an important role in the plankton communities of the Arctic and boreal seas, often dominating in numbers and constituting a significant proportion of the biomass of zooplankton. Despite their high presence and significance in the shelf plankton communities, species-specific studies of the biology of these are significantly hampered by extremely small morphological differences between them, especially at the juvenile stages, at which they are virtually indistinguishable. In this paper, we describe a new, routine and low-cost molecular method for identifying all Pseudocalanus species found in the Atlantic sector of the Arctic: the Arctic P. acuspes, P. minutus and the boreal P. moultoni and P. elongatus, and apply it to describe the relative distribution of these species in four locations of the Arctic and sub-Arctic. With this method, species-specific polymerase chain reaction (ssPCR), mass identification of individuals of any developmental stage, including nauplii, is possible. This method can serve as an excellent tool for studying the species-specific biology of this group, describing their life cycles, as well as monitoring changes in Arctic marine ecosystems under the influence of changing climate.

One-Step Directional Cloning of Blunt-Ended Polymerase Chain Reaction Products

2000 ◽  
Vol 287 (2) ◽  
pp. 349-352 ◽  
Author(s):  
Vincent W.S. Liu ◽  
Phillip Nagley ◽  
Hextan Y.S. Ngan
Keyword(s):  
Polymerase Chain Reaction ◽  
Reaction Products ◽  
Chain Reaction ◽  
Directional Cloning ◽  
One Step ◽  
Polymerase Chain
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