Hypersensitivity to ionizing radiation, in vitro, in a new chromosomal breakage disorder, the Nijmegen Breakage syndrome

1983 ◽  
Vol 112 (1) ◽  
pp. 23-32 ◽  
Author(s):  
R.D.F.M. Taalman ◽  
N.G.J. Jaspers ◽  
J.M.J.C. Scheres ◽  
J. de Wit ◽  
T.W.J. Hustinx
Keyword(s):  
Ionizing Radiation ◽  
Nijmegen Breakage Syndrome ◽  
Chromosomal Breakage

scholarly journals Phosphorylation of SDT repeats in the MDC1 N terminus triggers retention of NBS1 at the DNA damage–modified chromatin

2008 ◽  
Vol 181 (2) ◽  
pp. 213-226 ◽  
Author(s):  
Fredrik Melander ◽  
Simon Bekker-Jensen ◽  
Jacob Falck ◽  
Jiri Bartek ◽  
Niels Mailand ◽  
...  
Keyword(s):  
Adaptor Protein ◽  
Nijmegen Breakage Syndrome ◽  
Local Concentration ◽  
Dna Double Strand Breaks ◽  
Phosphate Binding ◽  
Chromosomal Breakage ◽  
Mrn Complex ◽  
N Terminus

DNA double-strand breaks (DSBs) trigger accumulation of the MRE11–RAD50–Nijmegen breakage syndrome 1 (NBS1 [MRN]) complex, whose retention on the DSB-flanking chromatin facilitates survival. Chromatin retention of MRN requires the MDC1 adaptor protein, but the mechanism behind the MRN–MDC1 interaction is unknown. We show that the NBS1 subunit of MRN interacts with the MDC1 N terminus enriched in Ser-Asp-Thr (SDT) repeats. This interaction was constitutive and mediated by binding between the phosphorylated SDT repeats of MDC1 and the phosphate-binding forkhead-associated domain of NBS1. Phosphorylation of the SDT repeats by casein kinase 2 (CK2) was sufficient to trigger MDC1–NBS1 interaction in vitro, and MDC1 associated with CK2 activity in cells. Inhibition of CK2 reduced SDT phosphorylation in vivo, and disruption of the SDT-associated phosphoacceptor sites prevented the retention of NBS1 at DSBs. Together, these data suggest that phosphorylation of the SDT repeats in the MDC1 N terminus functions to recruit NBS1 and, thereby, increases the local concentration of MRN at the sites of chromosomal breakage.

scholarly journals Ionizing radiation affects the composition of the proteome of extracellular vesicles released by head-and-neck cancer cells in vitro

2019 ◽  
Vol 60 (3) ◽  
pp. 289-297 ◽  
Author(s):  
Agata Abramowicz ◽  
Anna Wojakowska ◽  
Lukasz Marczak ◽  
Malgorzata Lysek-Gladysinska ◽  
Mateusz Smolarz ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Ionizing Radiation ◽  
Head And Neck ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Neck Cancer

scholarly journals A novel in vitro survival assay of small intestinal stem cells after exposure to ionizing radiation

2014 ◽  
Vol 55 (2) ◽  
pp. 381-390 ◽  
Author(s):  
Motohiro Yamauchi ◽  
Kensuke Otsuka ◽  
Hisayoshi Kondo ◽  
Nobuyuki Hamada ◽  
Masanori Tomita ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ionizing Radiation ◽  
Intestinal Stem Cells ◽  
Survival Assay ◽  
Small Intestinal ◽  
In Vitro Survival

In Vitro Bioenergetic Changes Associated with Ionizing Radiation (IR), Genotoxins and Redox Toxins

2014 ◽  
Vol 76 ◽  
pp. S135
Author(s):  
Brett Alan Wagner ◽  
Claire M Doskey ◽  
Juan Du ◽  
Joseph J Cullen ◽  
Garry R Buettner
Keyword(s):  
Ionizing Radiation

scholarly journals A study of the effects of therapeutic doses of ionizing radiation in vitro on Lactobacillus isolates originating from the vagina - a pilot study

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Tomasz Gosiewski ◽  
Tomasz Mróz ◽  
Dorota Ochońska ◽  
Wojciech Pabian ◽  
Malgorzata Bulanda ◽  
...  
Keyword(s):  
Pilot Study ◽  
Ionizing Radiation ◽  
Therapeutic Doses
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