Calcification of cartilage matrix in chondrocyte cultures derived from rachitic rat growth plate cartilage

1983 ◽  
Vol 5 (2) ◽  
pp. 87-92 ◽  
Author(s):  
H.K. Väänänen ◽  
D.C. Morris ◽  
H.C. Anderson
Keyword(s):  
Growth Plate ◽  
Cartilage Matrix ◽  
Growth Plate Cartilage ◽  
Chondrocyte Cultures ◽  
Rat Growth

Effect of sodium fluoride (F) on rat growth plate cartilage (GPC)

Bone ◽  
2015 ◽  
Vol 71 ◽  
pp. 258
Author(s):  
B.L. Fina ◽  
S.M. Roma ◽  
F. Bues ◽  
V.E. Di Loreto
Keyword(s):  
Growth Plate ◽  
Sodium Fluoride ◽  
Growth Plate Cartilage ◽  
Rat Growth

Expression of Thyroid Hormone Receptor Isoforms in Rat Growth Plate Cartilage In Vivo

1999 ◽  
Vol 14 (9) ◽  
pp. 1550-1556 ◽  
Author(s):  
R. Tracy Ballock ◽  
Barry C. Mita ◽  
Xiaolan Zhou ◽  
Daniel H.-C. Chen ◽  
Lynn M. Mink
Keyword(s):  
Thyroid Hormone ◽  
Growth Plate ◽  
Hormone Receptor ◽  
Thyroid Hormone Receptor ◽  
Growth Plate Cartilage ◽  
Receptor Isoforms ◽  
Rat Growth

A Fine Structural Study of Mineralization Foci in Antler and Growth Plate Cartilage

1975 ◽  
Vol 33 ◽  
pp. 482-483
Author(s):  
J. W. Newbrey ◽  
W. J. Banks
Keyword(s):  
Growth Plate ◽  
Uranyl Acetate ◽  
Ruthenium Red ◽  
Growth Plate Cartilage ◽  
Transmission Electron ◽  
Cacodylate Buffer ◽  
Rat Growth ◽  
Staining Techniques ◽  
Relationship Of ◽  
Hydroxyapatite Crystals

Established staining techniques for glycosaminoglycans (GAG) and established demineralization techniques were employed to investigate the possible relationship of GAG's to hydroxyapatite crystals in mineralized cartilage. Cervine antler cartilage and rat growth plate cartilage were subjected to glutaraldehyde (6.25%) fixation, demineralized with ethylene diaminotetra acetic acid (EDTA) or acidic cacodylate buffer (pH 5.5), stained and postfixed with a ruthenium red (RR)-osmium tetroxide solution and prepared for routine transmission electron microscopy. Some tissues were subjected to hyaluronidase digestion prior to RR staining. Sections were stained with RR only or RR and uranyl acetate. The sections were examined with a Hitachi HS-8 electron microscope.Matrix granules were positive to RR staining (Fig. 2&3). The granular density of the interterritorial zone was higher than that of the lacunar area; however, the lacunar granules were larger. After EDTA treatment, mineralization foci in the interterritorial zone were devoid of hydroxyapatite crystals.

Improved structural preservation of high-pressure frozen cartilage

1993 ◽  
Vol 51 ◽  
pp. 108-109
Author(s):  
Daniel Studer ◽  
Jeannine Wagner ◽  
Ernst B. Hunziker
Keyword(s):  
Extracellular Matrix ◽  
High Pressure ◽  
High Resolution ◽  
Growth Plate ◽  
High Resolution Imaging ◽  
Chemical Fixation ◽  
Growth Plate Cartilage ◽  
Rat Growth ◽  
Resolution Imaging ◽  
Structural Preservation

Adult cartilage is a unique tissue in that it is avascular and lacks innervation. The chondrocytes are embedded in an extracellular matrix which in most cases occupies 60 to 90% of the tissue volume. The ultrastructure and composition of rat growth plate cartilage has been investigated (1). It was found that during conventional chemical fixation of me tissue (2), about 60% of the extracellular matrix proteoglycans were lost (3). This artefact can be prevented if proteoglycans are precipitated by cationic dyes (eg. ruthenium hexaamine trichloride) during fixation (4) which forms relatively large precipitates by rendering high resolution imaging impossible. High pressure frozen extracellular matrix revealed a fine meshwork which was attributed to proteoglycan distribution (5). A meshwork due to segregation during freezing was not seen in recent experiments where adaequately frozen cartilage was investigated.200μm thick sections of rat growth plate cartilage were excised in a bath of hexadecene (6). The sections were again processed under hexadecene, firstly punched (diameter 1.7mm) and then transferred to an aluminum sandwhich (corresponding in size to the sample).

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