Epitope-specific antibody responses in market-stressed calves to bovine herpesvirus type 1

The potential for plasmids encoding a secreted form of bovine herpesvirus type 1 (BHV-1) glycoprotein D (gD) to elicit immune responses in passively immune mice following intramuscular immunization was investigated. In these experiments, 6- to 8-week-old female C3H/HeN or C57BL/6 mice were passively immunized with hyperimmune antisera raised against BHV-1 recombinant, truncated (secreted) gD immediately prior to immunization with plasmids. A single immunization of passively immune mice with plasmid encoding the secreted form of BHV-1 gD resulted in rapid development of both cell-mediated immunity and antibody responses. Furthermore, 50% of mice immunized with a suboptimal dose of recombinant gD formulated into an adjuvant developed significant levels of serum antibodies if mice were pre-treated with hyperimmune antisera. The apparent failure of passive polyclonal antisera to suppress the induction of immune responses to pSLRSV may be related to the immunoglobulin subtypes present in the hyperimmune sera.

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Secretory expression of bovine herpesvirus type 1/5 glycoprotein E in Pichia pastoris for the differential diagnosis of vaccinated or infected cattle

Protein Expression and Purification ◽

10.1016/j.pep.2016.09.018 ◽

2017 ◽

Vol 130 ◽

pp. 21-27

Author(s):

Bianca S. Siedler ◽

Bárbara C. Roloff ◽

Gizele L. de Sá ◽

Alessandra Neis ◽

Fabrício R. Conceição ◽

...

Keyword(s):

Differential Diagnosis ◽

Pichia Pastoris ◽

Bovine Herpesvirus ◽

Infected Cattle ◽

Secretory Expression ◽

Glycoprotein E ◽

Herpesvirus Type ◽

Bovine Herpesvirus Type 1

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Risk assessment and seroprevalence of bovine herpesvirus type 1 infection in dairy herds in the inner Aegean Region of Turkey

Comparative Immunology Microbiology and Infectious Diseases ◽

10.1016/j.cimid.2021.101741 ◽

2021 ◽

pp. 101741

Author(s):

Ömer Barış İnce ◽

Murat Şevik

Keyword(s):

Risk Assessment ◽

Bovine Herpesvirus ◽

Dairy Herds ◽

Aegean Region ◽

Herpesvirus Type ◽

Bovine Herpesvirus Type 1

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The bovine herpesvirus type 1 envelope protein Us9 acidic domain is crucial for anterograde axonal transport

Veterinary Microbiology ◽

10.1016/j.vetmic.2011.05.012 ◽

2011 ◽

Vol 152 (3-4) ◽

pp. 270-279 ◽

Cited By ~ 6

Author(s):

S.I. Chowdhury ◽

M.C.S. Brum ◽

C. Coats ◽

A. Doster ◽

Huiyong Wei ◽

...

Keyword(s):

Axonal Transport ◽

Envelope Protein ◽

Bovine Herpesvirus ◽

Herpesvirus Type ◽

Acidic Domain ◽

Anterograde Axonal Transport ◽

Bovine Herpesvirus Type 1

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Characterization of Monoclonal Antibodies against Bovine herpesvirus type 1 selected by Phage Display Technology

Semina Ciências Agrárias ◽

10.5433/1679-0359.2017v38n6p3915 ◽

2017 ◽

Vol 38 (6) ◽

pp. 3915

Author(s):

Greice Japolla ◽

Ana Flávia Batista Penido ◽

Greyciele Rodrigues Almeida ◽

Luiz Artur Mendes Bataus ◽

Jair Pereira Cunha Junior ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Phage Display ◽

Bovine Herpesvirus ◽

Single Chain ◽

Phage Display Technology ◽

Herpesvirus Type ◽

Wide Range ◽

Display Technology ◽

Bovine Herpesvirus Type 1

The specificity of monoclonal antibodies (mAbs) to desired targets makes these molecules suitable for therapeutic and diagnostic uses against a wide range of pathogens. Phage display antibody libraries offer one method by which mAbs can be selected for, without the use of conventional hybridoma technology. In this work, phage display technology was used to construct, select and characterize a combinatorial single chain fragment variable (scFv) antibody library against bovine herpesvirus type 1 (BoHV-1) from the immune repertoire of chickens immunized with the virus. In silico analysis of the hypervariable domains of the antibody heavy chains revealed a high frequency of scFv fragments with low variability, suggesting that selection had probably been carried out and favored by a few im-munogenic viral antigens. The reactivity of the scFv fragments selected against BoHV-1 was demon-strated by Phage-ELISA. A significant increase in antibody reactivity to the target was observed after six rounds of library selection, showing its potential use as a molecule for BoHV-1 diagnosis. The strategy described here opens up a field for the use of phage display as a tool for selection of mono-clonal antibodies that could be used for theranostic applications against infectious and parasitic dis-eases of veterinary interest.

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