Evolution of clonal heterogeneity of aneuploid cell populations in carcinoma in situ of the bladder investigated by flow cytometry

1988 ◽  
Vol 24 (9) ◽  
pp. 1550
Keyword(s):  
Flow Cytometry ◽  
Carcinoma In Situ ◽  
Cell Populations ◽  
Aneuploid Cell ◽  
Clonal Heterogeneity

Possibile ruolo della citofluorometria (CFM) nel monitoraggio della risposta alla terapia endocavitaria con BCG nel carcinoma in situ della vescica: Monitoring the response to intravesical BCG in the treatment of cis: A possible role of flow cytometry

1995 ◽  
Vol 62 (2) ◽  
pp. 245-247
Author(s):  
M. De Siati ◽  
D. Grassi ◽  
N. Franzolin ◽  
L.S. Azzolina
Keyword(s):  
Flow Cytometry ◽  
Carcinoma In Situ ◽  
Recurrent Disease ◽  
Intravesical Therapy ◽  
Urinary Cytology ◽  
Bacillus Calmette Guerin ◽  
Intravesical Bcg

We report our experience on the treatment of carcinoma in situ (CIS) using intravesical therapy with the Bacillus Calmette-Guerin (BCG). From November 1992 to September 1994, 18 patients received treatment: 6 had associated CIS and 12 secondary CIS. Ploidy of each tumour was determined by flow cytometry. Aneuploidy was found in 12 cases, diploidy in 6 cases. After treatment, a standard bladder mapping was performed: 14 patients showed no evidence of cystoscopic and histological disease and if previously aneuploid, became diploid. 4 patients has recurrent disease after therapy; they were all aneuploid before treatment. One of these showed a persistent aneuploidy, although both voided urinary cytology and histological samples were negative. Six months later, a recurrent CIS was seen at the time of cystoscopy. These results enhance the interest in flow cytometry as a possible predictor of response to BCG in the treatment of CIS.

scholarly journals Changes in immune Cell Types in Breast Suggest a Decline in Immune Surveillance and Increased Immunosuppression with Age

2021 ◽  
Author(s):  
Arrianna Zirbes ◽  
Jesuchristopher Joseph ◽  
Jennifer C Lopez ◽  
Rosalyn W Sayaman ◽  
Mudaser Basam ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
Epithelial Cell ◽  
Peripheral Blood ◽  
Breast Tissue ◽  
Immune Cell ◽  
Normal Breast Tissue ◽  
Normal Breast ◽  
Cell Populations

Abstract Background : A majority of breast cancers (BC) are age-related and we seek to determine what cellular and molecular changes occur in breast tissue with age that make women more susceptible to cancer initiation. Immune-epithelial cell interactions are important during mammary gland development and the immune system plays an important role in BC progression. The composition of human immune cell populations is known to change in peripheral blood with age and in breast tissue during BC progression. Less is known about changes in immune populations in normal breast tissue and how their interactions with mammary epithelia change with age. Methods : We quantified densities of T cells, B cells, and macrophage subsets in pathologically normal breast tissue from 122 different women who ranged in age from 24 to 74 years old. Donor-matched peripheral blood from a subset of 20 donors was analyzed by flow cytometry. Tissue immune cell densities and localizations relative to the epithelium were quantified in situ with machine learning-based analyses of multiplex immunohistochemistry-stained tissue sections. In situ results were corroborated with flow cytometry analyses of peri-epithelial immune cells from primary organoid preparations and transcriptome analyses of public data from bulk tissue reduction mammoplasties. Results : Proportions of immune cell subsets in breast tissue and donor-matched peripheral blood were not correlated. Density (cells/mm 2 ) of T and B lymphocytes in situ decreased with age. T cells and macrophages preferentially localized near or within epithelial bilayers, rather than the intralobular stroma. M2:M1 macrophage ratio increased with age and was accompanied by an increased density of M2 in the intralobular stroma. Transcriptional signature analyses suggested age-dependent decline in adaptive immune cell populations and functions and increased innate immune cell activity. Conclusions : T cells and macrophages are so intimately associated with the epithelia that they are embedded within the bilayer, suggesting an important role for immune-epithelial cell interactions. Age-associated decreased T cell density in peri-epithelial regions, and increased M2 macrophage density in intralobular stroma suggests the emergence of a tissue microenvironment that is simultaneously immune-senescent and immunosuppressive with age .

scholarly journals Analysis of Gene Expression Profiles of Microdissected Cell Populations Indicates that Testicular Carcinoma In situ Is an Arrested Gonocyte

2009 ◽  
Vol 69 (12) ◽  
pp. 5241-5250 ◽  
Author(s):  
Si Brask Sonne ◽  
Kristian Almstrup ◽  
Marlene Dalgaard ◽  
Agnieszka Sierakowska Juncker ◽  
Daniel Edsgard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Carcinoma In Situ ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Cell Populations ◽  
Testicular Carcinoma

Carcinoma in situ of the testis detected by DNA flow cytometry of testicular fine-needle aspirates

Cytometry ◽  
1994 ◽  
Vol 17 (4) ◽  
pp. 327-331 ◽  
Author(s):  
Anton Hittmair ◽  
Hermann Rogtsch ◽  
Hans Feichtinger ◽  
Alfred Hobisch ◽  
Gregor Mikuz
Keyword(s):  
Flow Cytometry ◽  
Carcinoma In Situ ◽  
Dna Flow Cytometry ◽  
Fine Needle ◽  
Fine Needle Aspirates

Aneuploid and Polyploid Cellular DNA Heterogeneity in Insect Cell Material of Diptera Species Analyzed by Flow Cytometry

1990 ◽  
Vol 45 (9-10) ◽  
pp. 1027-1030 ◽  
Author(s):  
Wolfgang Ulrich
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Insect Cell ◽  
Dna Analysis ◽  
Calliphora Vicina ◽  
Polyploid Cell ◽  
Cell Populations ◽  
Aneuploid Cell ◽  
Flow Cytometric ◽  
Cell Material

Abstract Flow cytometric 1-parameter DNA analysis and 2-parameter DNA /protein analysis have been performed with cell material of the diptera species Chironomus thummi, Drosophila melanogaster, Calliphora vicina and Musca domestica using an impulse cytophotometer with a new quartz objective, that was especially manufactured for cytofluorometric investigations. The occurrence of heterogenous cell populations with aneuploid and polyploid DNA content within the cell material of different developmental stages of diptera species have been determined, whereby in larvae polyploid cell populations and in imagos aneuploid cell populations predominate. Partially separation of 2C cells from other cell populations with higher DNA content can be done by Ficoll-Hypaque centrifugation as demonstrated with cell material from Chironomus larvae. For flow cytometric DNA analysis of insect cell material a simple and rapid cell preparation and staining technique is presented by using the DNA-specific fluorochrome DAPI in combination with the protein fluorochrome sulforhodamine 101. Employment of flow cytometry in diptera genetics might be a new tool for cytological and cytogenetic investigations as shown with the classical genetic objects Chironomus and Drosophila

1252: Impact of Concomitant Carcinoma in situ on Clinical Outcomes after Radical Cystectomy

2006 ◽  
Vol 175 (4S) ◽  
pp. 403-403 ◽  
Author(s):  
Shahrokh F. Shariat ◽  
Ganesh S. Palapattu ◽  
Gilad E. Amiel ◽  
Pierre I. Karakiewicz ◽  
Craig G. Rogers ◽  
...  
Keyword(s):  
Clinical Outcomes ◽  
Radical Cystectomy ◽  
Carcinoma In Situ

Prognoseverbesserung beim DCIS: Weniger invasive Rezidive durch adjuvante Therapien

2011 ◽  
Vol 02 (03) ◽  
pp. 110-110
Author(s):  
Katharina Arnheim
Keyword(s):  
Carcinoma In Situ

Patientinnen mit duktalem Carcinoma in situ (DCIS) müssen nicht mastektomiert werden; sie haben auch nach brusterhaltender Operation ein günstiges Outcome. Eine weitere Prognoseverbesserung wird durch Nachbestrahlung und adjuvante Tamoxifen-Gabe erreicht.

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