Wobble position modified nucleosides evolved to select transfer RNA codon recognition: A modified-wobble hypothesis

Biochimie ◽  
1991 ◽  
Vol 73 (11) ◽  
pp. 1345-1349 ◽  
Author(s):  
P AGRIS
Keyword(s):  
Transfer Rna ◽  
Modified Nucleosides ◽  
Codon Recognition ◽  
Wobble Position ◽  
Wobble Hypothesis

scholarly journals Inosine in Biology and Disease

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 600
Author(s):  
Sundaramoorthy Srinivasan ◽  
Adrian Gabriel Torres ◽  
Lluís Ribas de Pouplana
Keyword(s):  
Human Health ◽  
Purine Biosynthesis ◽  
Messenger Rnas ◽  
Transfer Rnas ◽  
Functional Roles ◽  
Codon Recognition ◽  
Wobble Position ◽  
Biosynthesis Gene ◽  
The Stability ◽  
Cell Signaling Pathways

The nucleoside inosine plays an important role in purine biosynthesis, gene translation, and modulation of the fate of RNAs. The editing of adenosine to inosine is a widespread post-transcriptional modification in transfer RNAs (tRNAs) and messenger RNAs (mRNAs). At the wobble position of tRNA anticodons, inosine profoundly modifies codon recognition, while in mRNA, inosines can modify the sequence of the translated polypeptide or modulate the stability, localization, and splicing of transcripts. Inosine is also found in non-coding and exogenous RNAs, where it plays key structural and functional roles. In addition, molecular inosine is an important secondary metabolite in purine metabolism that also acts as a molecular messenger in cell signaling pathways. Here, we review the functional roles of inosine in biology and their connections to human health.

Preferences of AAA/AAG codon recognition by modified nucleosides, τm5s2U34 and t6A37 present in tRNALys

2017 ◽  
Vol 36 (16) ◽  
pp. 4182-4196 ◽  
Author(s):  
Kailas D. Sonawane ◽  
Asmita S. Kamble ◽  
Prayagraj M. Fandilolu
Keyword(s):  
Modified Nucleosides ◽  
Codon Recognition

Thiolation of uridine carbon-2 restricts the motional dynamics of the transfer RNA wobble position nucleoside

1992 ◽  
Vol 114 (7) ◽  
pp. 2652-2656 ◽  
Author(s):  
Paul F. Agris ◽  
Hanna Sierzputowska-Gracz ◽  
Wanda Smith ◽  
A. Malkiewicz ◽  
Elzbieta Sochacka ◽  
...  
Keyword(s):  
Transfer Rna ◽  
Wobble Position ◽  
Motional Dynamics

Posttranscriptionally modified nucleosides in transfer RNA: Their locations and frequencies

Biochimie ◽  
1995 ◽  
Vol 77 (1-2) ◽  
pp. 139-141 ◽  
Author(s):  
H. Grosjean ◽  
M. Sprinzl ◽  
S. Steinberg
Keyword(s):  
Transfer Rna ◽  
Modified Nucleosides

scholarly journals Absolute requirement for polyamines for growth of Escherichia coli mutants (mnmE/G) defective in modification of the wobble anticodon of transfer-RNA

2019 ◽  
Vol 366 (10) ◽  
Author(s):  
Christopher Keller ◽  
Manas Chattopadhyay ◽  
Herbert Tabor
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Protein Biosynthesis ◽  
Transfer Rna ◽  
Coli Strain ◽  
E Coli ◽  
Wobble Position ◽  
Inhibition Of Growth ◽  
Absolute Requirement

Abstract The genes mnmE and mnmG are responsible for the modification of uridine 34, ‘the wobble position’ of many aminoacyl-tRNAs. Deletion of these genes affects the strength of the codon-anticodon interactions of the aminoacyl-tRNAs with the mRNAs and the ribosomes. However, deletion of these genes does not usually have a significant effect on the growth rate of the standard Escherichia coli strains. In contrast, we have found that if the host E. coli strain is deficient in the synthesis of polyamines, deletion of the mnmE or mnmG gene results in complete inhibition of growth unless the medium contains polyamines. The finding of an absolute requirement for polyamines in our current work will be significant in studies on polyamine function, in studies on the function of the mnmE/G genes, and in studies on the role of aminoacyl-tRNAs in protein biosynthesis.

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