scholarly journals Inosine in Biology and Disease

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 600
Author(s):  
Sundaramoorthy Srinivasan ◽  
Adrian Gabriel Torres ◽  
Lluís Ribas de Pouplana
Keyword(s):  
Human Health ◽  
Purine Biosynthesis ◽  
Messenger Rnas ◽  
Transfer Rnas ◽  
Functional Roles ◽  
Codon Recognition ◽  
Wobble Position ◽  
Biosynthesis Gene ◽  
The Stability ◽  
Cell Signaling Pathways

The nucleoside inosine plays an important role in purine biosynthesis, gene translation, and modulation of the fate of RNAs. The editing of adenosine to inosine is a widespread post-transcriptional modification in transfer RNAs (tRNAs) and messenger RNAs (mRNAs). At the wobble position of tRNA anticodons, inosine profoundly modifies codon recognition, while in mRNA, inosines can modify the sequence of the translated polypeptide or modulate the stability, localization, and splicing of transcripts. Inosine is also found in non-coding and exogenous RNAs, where it plays key structural and functional roles. In addition, molecular inosine is an important secondary metabolite in purine metabolism that also acts as a molecular messenger in cell signaling pathways. Here, we review the functional roles of inosine in biology and their connections to human health.

Selection for soyabeans with high and environmentally stable lutein concentrations

2014 ◽  
Vol 12 (S1) ◽  
pp. S12-S16 ◽  
Author(s):  
Krishna Hari Dhakal ◽  
Myoung-Gun Choung ◽  
Young-Sun Hwang ◽  
Felix B. Fritschi ◽  
J. Grover Shannon ◽  
...  
Keyword(s):  
Human Health ◽  
High Stability ◽  
Planting Dates ◽  
Early Maturity ◽  
Breeding Objective ◽  
Late Maturity ◽  
Selection For ◽  
High Concentrations ◽  
The Stability ◽  
Nutritional Benefits

Lutein has significant nutritional benefits for human health. Therefore, enhancing soybean lutein concentrations is an important breeding objective. However, selection for soybeans with high and environmentally stable lutein concentrations has been limited. The objectives of this study were to select soybeans with high seed lutein concentrations and to determine the stability of lutein concentrations across environments. A total of 314 genotypes were screened and 18 genotypes with high lutein concentrations and five genotypes with low lutein concentrations were selected for further examination. These 23 genotypes and two check varieties were evaluated under six environments (two planting dates for 2 years at one location and two planting dates for 1 year at another location). Lutein concentrations were influenced by genotype, environment and genotype × environment interactions. Genotypes with late maturity and low lutein concentrations were more stable than those with early maturity and high concentrations. Early (May) planting resulted in greater lutein concentrations than late (June) planting. Among the genotypes evaluated, PI603423B (7.7 μg/g) and PI89772 (5.8 μg/g) had the greatest mean lutein concentrations and exhibited medium and high stability across the six environments, respectively. Thus, these genotypes may be useful for breeding soybeans with high and stable seed lutein concentrations.

CONSTANT AND VARIABLE IN THE STYLE OF A RUSSIAN PIANO QUARTET

2020 ◽  
Vol 1 (2) ◽  
pp. 69-74
Author(s):  
N. K. Samoilova ◽  
Keyword(s):  
Chamber Music ◽  
New Techniques ◽  
Functional Roles ◽  
Russian Music ◽  
Piano Quartet ◽  
Music Making ◽  
The Difference ◽  
The Stability ◽  
Transformation Processes ◽  
Different Levels

The process of the steady rooting of invariant genre features of a piano quartet is considered through the ratio between stable and mobile features. Here there is either the complete independence of the instruments, but on the principles of equal participation in the embodiment of musical content, or the variability in the relations of instrumental parts. As the main ones in the genre, the musical norms of the classic-romantic style and the stability of the timbre composition are established. It is noted that in Russian music the evolution of the piano quartet passed through different phases: genre stabilization and recovery, temporary stop and subsequent active development. Transformation processes went through different levels: structure, content, instrumental- timbre solutions, rethinking the functional roles of partner instruments. The movement from the traditional normativity of instrumental compositions to the difference of timbre combinations in the XXth–XXIst centuries was primarily predetermined by such factors as polyphonization of texture, the active introduction of polyphonic forms, new techniques of instrumentation. In the modern piano quartet, the central idea of chamber music making, the idea of co-creation generates both an extremely individualized form of embodiment and a free timbre composition. In conclusion, it is noted that the piano quartet genre has the ability to accumulate the leading style trends in chamber music of different eras.

ROLE OF THE POLYADENYLATE SEGMENT IN THE STABILITY OF EUKARYOTIC MESSENGER RNAs

Gene Function ◽  
1979 ◽  
pp. 427-436 ◽  
Author(s):  
G. Marbaix ◽  
G. Huez ◽  
E. Hubert ◽  
Y. Cleuter ◽  
H. Soreq ◽  
...  
Keyword(s):  
Messenger Rnas ◽  
The Stability

Is protein context responsible for peptide-mediated interactions?

2019 ◽  
Vol 15 (4) ◽  
pp. 280-295 ◽  
Author(s):  
Peng Zhou ◽  
Qingqing Miao ◽  
Fugang Yan ◽  
Zhongyan Li ◽  
Qianhu Jiang ◽  
...  
Keyword(s):  
Cell Signaling ◽  
Signaling Pathways ◽  
The Stability ◽  
Cell Signaling Pathways

Many cell signaling pathways are orchestrated by the weak, transient, and reversible peptide-mediated interactions (PMIs). Here, the role of protein context in contributing to the stability and specificity of PMIs is investigated systematically.

scholarly journals Degradation of the Transcription Factor Gcn4 Requires the Kinase Pho85 and the SCFCDC4 Ubiquitin–Ligase Complex

2000 ◽  
Vol 11 (3) ◽  
pp. 915-927 ◽  
Author(s):  
Ariella Meimoun ◽  
Tsvi Holtzman ◽  
Ziva Weissman ◽  
Helen J. McBride ◽  
David J. Stillman ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Transcription Factor ◽  
Ubiquitin Ligase ◽  
Protein A ◽  
Purine Biosynthesis ◽  
Cell Cycle Regulators ◽  
Rich Medium ◽  
Ubiquitin Ligase Complex ◽  
Ubiquitin Conjugating Enzyme ◽  
The Stability

Gcn4, a yeast transcriptional activator that promotes the expression of amino acid and purine biosynthesis genes, is rapidly degraded in rich medium. Here we report that SCFCDC4, a recently characterized protein complex that acts in conjunction with the ubiquitin-conjugating enzyme Cdc34 to degrade cell cycle regulators, is also necessary for the degradation of the transcription factor Gcn4. Degradation of Gcn4 occurs throughout the cell cycle, whereas degradation of the known cell cycle substrates of Cdc34/SCFCDC4 is cell cycle regulated. Gcn4 ubiquitination and degradation are regulated by starvation for amino acids, whereas the degradation of the cell cycle substrates of Cdc34/SCFCDC4 is unaffected by starvation. We further show that unlike the cell cycle substrates of Cdc34/SCFCDC4, which require phosphorylation by the kinase Cdc28, Gcn4 degradation requires the kinase Pho85. We identify the critical target site of Pho85 on Gcn4; a mutation of this site stabilizes the protein. A specific Pho85-Pcl complex that is able to phosphorylate Gcn4 on that site is inactive under conditions under which Gcn4 is stable. Thus, Cdc34/SCFCDC4 activity is constitutive, and regulation of the stability of its various substrates occurs at the level of their phosphorylation.

IDENTIFICATION AND CHARACTERIZATION OF PIGMENTED BACTERIA ISOLATED FROM MALAYSIAN SEAWATER

2019 ◽  
Vol 7 (4) ◽  
pp. 01-08
Author(s):  
Nur Afifah Mursyida Zaujan ◽  
Mohamad Zohdi Othman ◽  
Fatin Najihah Mohd Lutfi ◽  
Kamarul Rahim Kamarudin ◽  
Hanina Mohd Noor ◽  
...  
Keyword(s):  
Human Health ◽  
Pcr Amplification ◽  
Fluorescent Light ◽  
Rrna Gene ◽  
Yellow Orange ◽  
Exiguobacterium Profundum ◽  
The Stability ◽  
Antimicrobial Metabolites ◽  
Pigmented Bacteria

Purpose of study: Bacteria can naturally produce pigments that can be useful for various applications as they possess antimicrobial metabolites among other numerous benefits towards the human health. This study was carried out to identify the species of marine bacterial isolates PMA, PM3C1 and PM5C1 exhibiting yellow, orange and green colors respectively. Methodology: The current study is using Polymerase Chain Reaction (PCR) amplification and sequence analysis of their 16S rRNA gene. The stability of pigments extracted from the bacterial samples was also analyzed against different temperature and light conditions. Main Findings: Sequence alignment using BLAST revealed that the yellow, orange, and green-pigmented bacteria have 84% similarity with Staphylococcus aureus, 85% similarity with Exiguobacterium profundum and 95% similarity with Pseudomonas aeruginosa respectively. The green pigment showed major changes in color following exposure to sunlight and fluorescent light, and when incubated at 24°C and 50°C. Exposure to direct sunlight also results in the reduction of color for the yellow and orange extracts, while no effect was observed for both pigments under fluorescent light. Incubation at 50°C results in the reduction of the orange color, while the yellow pigment was observed to be unaffected suggesting its stability at high temperature. Implications: Natural pigments production can provide many advantages including reduction of pollution generation, ease of disposal and other benefits to the human health.

scholarly journals Plasmid location of Borrelia purine biosynthesis gene homologs.

1994 ◽  
Vol 176 (21) ◽  
pp. 6427-6432 ◽  
Author(s):  
N Margolis ◽  
D Hogan ◽  
K Tilly ◽  
P A Rosa
Keyword(s):  
Purine Biosynthesis ◽  
Biosynthesis Gene

scholarly journals A Timely Review of Cross-Kingdom Regulation of Plant-Derived MicroRNAs

2021 ◽  
Vol 12 ◽  
Author(s):  
Dan Li ◽  
Jianhui Yang ◽  
Yong Yang ◽  
Jianxin Liu ◽  
Hui Li ◽  
...  
Keyword(s):  
Immune Modulation ◽  
Current Knowledge ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Messenger Rnas ◽  
Plant Mirnas ◽  
Complementary Oligonucleotide ◽  
Density Lipoprotein Receptor ◽  
The Cross ◽  
The Stability

MicroRNAs (miRNAs) belong to a class of non-coding RNAs that suppress gene expression by complementary oligonucleotide binding to the sites in target messenger RNAs. Numerous studies have demonstrated that miRNAs play crucial role in virtually all cellular processes of both plants and animals, such as cell growth, cell division, differentiation, proliferation and apoptosis. The study of rice MIR168a has demonstrated for the first time that exogenous plant MIR168a influences cholesterol transport in mice by inhibiting low-density lipoprotein receptor adapter protein 1 expression. Inspired by this finding, the cross-kingdom regulation of plant-derived miRNAs has drawn a lot of attention because of its capability to provide novel therapeutic agents in the treatment of miRNA deregulation-related diseases. Notably, unlike mRNA, some plant miRNAs are robust because of their 3′ end modification, high G, C content, and the protection by microvesicles, miRNAs protein cofactors or plant ingredients. The stability of these small molecules guarantees the reliability of plant miRNAs in clinical application. Although the function of endogenous miRNAs has been widely investigated, the cross-kingdom regulation of plant-derived miRNAs is still in its infancy. Herein, this review summarizes the current knowledge regarding the anti-virus, anti-tumor, anti-inflammatory, anti-apoptosis, immune modulation, and intestinal function regulation effects of plant-derived miRNAs in mammals. It is expected that exploring the versatile role of plant-derived miRNAs may lay the foundation for further study and application of these newly recognized, non-toxic, and inexpensive plant active ingredients.

scholarly journals How Many Messenger RNAs Can Be Translated by the START Mechanism?

2020 ◽  
Vol 21 (21) ◽  
pp. 8373
Author(s):  
Laurence Despons ◽  
Franck Martin
Keyword(s):  
Messenger Rna ◽  
Mrna Translation ◽  
Start Codon ◽  
Messenger Rnas ◽  
Coding Sequences ◽  
Coding Sequence ◽  
Rna Translation ◽  
Codon Recognition ◽  
G Quadruplex ◽  
Stable Structures

Translation initiation is a key step in the protein synthesis stage of the gene expression pathway of all living cells. In this important process, ribosomes have to accurately find the AUG start codon in order to ensure the integrity of the proteome. “Structure Assisted RNA Translation”, or “START”, has been proposed to use stable secondary structures located in the coding sequence to augment start site selection by steric hindrance of the progression of pre-initiation complex on messenger RNA. This implies that such structures have to be located downstream and at on optimal distance from the AUG start codon (i.e., downstream nucleotide +16). In order to assess the importance of the START mechanism in the overall mRNA translation process, we developed a bioinformatic tool to screen coding sequences for such stable structures in a 50 nucleotide-long window spanning the nucleotides from +16 to +65. We screened eight bacterial genomes and six eukaryotic genomes. We found stable structures in 0.6–2.5% of eukaryotic coding sequences. Among these, approximately half of them were structures predicted to form G-quadruplex structures. In humans, we selected 747 structures. In bacteria, the coding sequences from Gram-positive bacteria contained 2.6–4.2% stable structures, whereas the structures were less abundant in Gram-negative bacteria (0.2–2.7%). In contrast to eukaryotes, putative G-quadruplex structures are very rare in the coding sequence of bacteria. Altogether, our study reveals that the START mechanism seems to be an ancient strategy to facilitate the start codon recognition that is used in different kingdoms of life.

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