In vivo and in vitro growth-stimulatory effects of pigeon milk

In order to increase the availability of SCLC cells derived from biopsies, in vivo and in vitro growth methods were investigated. The cells grown in both conditions were periodically monitored for reactivity with 2 monoclonal antibodies (MAbs): MLuC1 directed against SCLC cells and IM1 which recognizes the class II antigen on activated lymphocytes and macrophages. About 50 % of the 28 analyzed SCLC specimens were found to proliferate in one or both systems. The in vitro-grown cells exhibited the same heterogeneity found in the original cell suspensions and moreover, in some cases only normal cells were recovered after several in vitro passages. From the subcutaneous transplanted tumors a large number of MLuC1-positive tumor cells could easily be recovered, thus indicating the validity of the in vivo methodology. The MBr1 MAb, directed against an epithelial antigen, was found to react with about 50 % of the 26 tested tumors, mainly those which demonstrated in vivo and/or in vitro growth capacity. These data suggest that only some tumors, presumably with peculiar biological characteristics, can efficiently grow in these artificial systems.

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Ophiobolin A, a sesterterpenoid fungal phytotoxin, displays higher in vitro growth-inhibitory effects in mammalian than in plant cells and displays in vivo antitumor activity

International Journal of Oncology ◽

10.3892/ijo.2013.1979 ◽

2013 ◽

Vol 43 (2) ◽

pp. 575-585 ◽

Cited By ~ 24

Author(s):

MARINA BURY ◽

ESTHER NOVO-UZAL ◽

ANNA ANDOLFI ◽

SARA CIMINI ◽

NATHALIE WAUTHOZ ◽

...

Keyword(s):

Antitumor Activity ◽

Plant Cells ◽

In Vitro Growth ◽

Inhibitory Effects ◽

Growth Inhibitory ◽

In Vivo Antitumor Activity

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In vitro growth stimulatory and in vivo wound healing studies on cycloartane-type saponins of Astragalus genus

Journal of Ethnopharmacology ◽

10.1016/j.jep.2011.01.030 ◽

2011 ◽

Vol 134 (3) ◽

pp. 844-850 ◽

Cited By ~ 39

Author(s):

Canan Sevimli-Gür ◽

İlyas Onbaşılar ◽

Pergin Atilla ◽

Rükan Genç ◽

Nur Çakar ◽

...

Keyword(s):

Wound Healing ◽

In Vitro Growth

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Glucan Is a Component of the Mycobacterium tuberculosis Surface That Is Expressed In Vitro and In Vivo

Infection and Immunity ◽

10.1128/iai.70.5.2566-2575.2002 ◽

2002 ◽

Vol 70 (5) ◽

pp. 2566-2575 ◽

Cited By ~ 41

Author(s):

J. Reid Schwebach ◽

Aharona Glatman-Freedman ◽

Leslie Gunther-Cummins ◽

Zhongdong Dai ◽

John B. Robbins ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Cell Envelope ◽

Tween 80 ◽

Enzyme Linked Immunosorbent Assay ◽

In Vitro Growth ◽

Comprehensive Picture ◽

The Media ◽

Surface Polysaccharide

ABSTRACT The outermost layer of Mycobacterium tuberculosis is composed primarily of two polysaccharides, glucan (GC) and arabinomannan. To analyze the surface polysaccharide composition of M. tuberculosis, we generated a monoclonal antibody (MAb) that binds M. tuberculosis GC and is known as MAb 24c5. Immunofluorescence and whole-mount immunoelectron microscopy indicated that GC is on the outermost portion of the bacteria. M. tuberculosis strains Erdman and CDC 1551 were analyzed for their ability to bind MAb 24c5 after in vitro growth in media with and without the detergent Tween 80. MAb 24c5 bound to Erdman and CDC 1551 at all culture times with only slightly greater apparent affinity after extended culture in the absence of Tween 80, indicating that a stable amount of GC polysaccharide antigen is associated with the cell surface of M. tuberculosis. An enzyme-linked immunosorbent assay indicated that GC is antigenically similar to glycogen, and the amount of GC antigen increased in the media of M. tuberculosis cultures grown either with or without the detergent Tween 80. Other nontuberculosis mycobacteria have antigenically similar GCs on their surfaces after in vitro growth. Inoculation of mice with live bacilli but not inoculation with dead bacilli elicited a strong antibody response to GC consistent with production of this antigen in vivo. Our results provide a more comprehensive picture of the M. tuberculosis cell envelope and the conditions that allow expression of M. tuberculosis GC.

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