Construction and evaluation of a frequency-domain epifluorescence microscope for lifetime and anisotropy decay measurements in subcellular domains

1991 ◽  
Vol 40 (1) ◽  
pp. 117-125 ◽  
Author(s):  
A.S. Verkman ◽  
Michael Armijo ◽  
Kiyohide Fushimi
Keyword(s):  
Frequency Domain ◽  
Anisotropy Decay ◽  
Subcellular Domains ◽  
Epifluorescence Microscope

Demonstration of an associated anisotropy decay by frequency-domain fluorometry

1987 ◽  
Vol 27 (3) ◽  
pp. 233-241 ◽  
Author(s):  
Henryk Szmacinski ◽  
Ranjith Jayaweera ◽  
Henryk Cherek ◽  
Joseph R. Lakowicz
Keyword(s):  
Frequency Domain ◽  
Anisotropy Decay ◽  
Frequency Domain Fluorometry

Anisotropie Rotation of Yt-Base in n-Propanol at – 20°C Studied by Frequency-Domain Fluorometry and Global Analysis

1991 ◽  
Vol 46 (3) ◽  
pp. 269-274
Author(s):  
Ignacy Gryczynski ◽  
S. Paszyc ◽  
Wieslaw M. Wiczk ◽  
Gabor Laczko ◽  
Nanda Joshi, ◽  
...  
Keyword(s):  
Frequency Response ◽  
Frequency Domain ◽  
Decay Time ◽  
Additional Data ◽  
Global Analysis ◽  
Correlation Times ◽  
Anisotropy Decay ◽  
Polarized Emission ◽  
The Mean ◽  
Frequency Domain Fluorometry

AbstractThe frequency response of the polarized emission of Yt-base in n-propanol at -20°C was measured. Excitation wavelengths of 346, 312 and 285 nm were used for which the fundamental anisotropics were 0.32, 0.19 and 0.04, respectively. Additional data were obtained using CCl4 to decrease the mean decay time from 10 ns to 0.7 ns. These nine sets of data were analyzed globally to recover the anisotropy decay law. Two correlation times, of about 146 ps and 502 ps, were needed for a good fit

Review of fluorescence anisotropy decay analysis by frequency-domain fluorescence spectroscopy

1993 ◽  
Vol 3 (2) ◽  
pp. 103-116 ◽  
Author(s):  
Joseph R. Lakowicz ◽  
Henryk Cherek ◽  
Józef Kuśba ◽  
Ignacy Gryczynski ◽  
Michael L. Johnson
Keyword(s):  
Fluorescence Spectroscopy ◽  
Frequency Domain ◽  
Fluorescence Anisotropy ◽  
Fluorescence Anisotropy Decay ◽  
Anisotropy Decay

Elucidating cyclic AMP signaling in subcellular domains with optogenetic tools and fluorescent biosensors

2019 ◽  
Vol 47 (6) ◽  
pp. 1733-1747 ◽  
Author(s):  
Christina Klausen ◽  
Fabian Kaiser ◽  
Birthe Stüven ◽  
Jan N. Hansen ◽  
Dagmar Wachten
Keyword(s):  
Cyclic Amp ◽  
Adenosine Monophosphate ◽  
Adenylyl Cyclases ◽  
Temporal Precision ◽  
Fluorescent Biosensors ◽  
Subcellular Domains ◽  
Spatio Temporal ◽  
Hydrolysis Of ◽  
Shed Light ◽  
Cyclic Amp Signaling

The second messenger 3′,5′-cyclic nucleoside adenosine monophosphate (cAMP) plays a key role in signal transduction across prokaryotes and eukaryotes. Cyclic AMP signaling is compartmentalized into microdomains to fulfil specific functions. To define the function of cAMP within these microdomains, signaling needs to be analyzed with spatio-temporal precision. To this end, optogenetic approaches and genetically encoded fluorescent biosensors are particularly well suited. Synthesis and hydrolysis of cAMP can be directly manipulated by photoactivated adenylyl cyclases (PACs) and light-regulated phosphodiesterases (PDEs), respectively. In addition, many biosensors have been designed to spatially and temporarily resolve cAMP dynamics in the cell. This review provides an overview about optogenetic tools and biosensors to shed light on the subcellular organization of cAMP signaling.

Correction to "The analysis of slug tests in the frequency domain" (WRR 25(11), pp. 2388-2396

1990 ◽  
Vol 26 (8) ◽  
pp. 1863-1863
Author(s):  
Paul Marschall ◽  
Baldur Barczewski
Keyword(s):  
Frequency Domain ◽  
Slug Tests

Methods of calibration of high-resolution optical reflectometers operating in the frequency domain

2018 ◽  
pp. 40-43
Author(s):  
V.V. GRIGORIEV ◽  
◽  
V.E. KRAVTSOV ◽  
A.K. MITUEV ◽  
E.A. MOROZ ◽  
...  
Keyword(s):  
High Resolution ◽  
Frequency Domain
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