Characterization of alveolar secretory cells from lactating cow mammary glands by Peanut Agglutinin (PNA)

1993 ◽  
Vol 35 (1-2) ◽  
pp. 185
Keyword(s):  
Mammary Glands ◽  
Secretory Cells ◽  
Peanut Agglutinin

scholarly journals Analysis of EGFR and HER-2 expressions in ductal carcinomas in situ in canine mammary glands

2014 ◽  
Vol 66 (3) ◽  
pp. 763-768 ◽  
Author(s):  
I.L.D. Silva ◽  
A.P.M. Dias ◽  
A.C. Bertagnolli ◽  
G.D. Cassali ◽  
E. Ferreira
Keyword(s):  
Ductal Carcinoma ◽  
Mammary Glands ◽  
Statistical Correlation ◽  
Low Grade ◽  
Neoplastic Progression ◽  
Her 2 ◽  
Epidermal Growth ◽  
Ductal Carcinomas

Biomolecular evidence has shown that ductal carcinoma in situ(DCIS) may develop into invasive carcinoma of the canine mammary gland, and mutations in proto-oncogenes HER2 and EGFR; two members of the family of epidermal growth factor receptors, may be involved in this process. The purpose of this study was the characterization of the immunohistochemical expression of the EGFR and HER2 proteins in the process of neoplastic transformation, supposedly present in ductal carcinomas in situin canine mammary glands. Fifteen cases of DCIS were evaluated, with a higher expression of HER2 and EGFR being observed in low-grade carcinomas when compared with high-grade neoplasms, and with a high positive statistical correlation in the latter. Results suggest that aggressive tumors tend to lose the expression of EGFR and HER2 simultaneously. The loss of the expression of these markers may be related to the process of neoplastic progression in canine mammary tumors.

scholarly journals Microscopical characterization of the salivary glands of the carnivorous cephalaspidean Philinopsis depicta (Mollusca, Opisthobranchia)

2009 ◽  
Vol 15 (S3) ◽  
pp. 39-40
Author(s):  
A. Lobo-da-Cunha ◽  
I. Ferreira ◽  
G. Calado
Keyword(s):  
Salivary Glands ◽  
Secretory Cells ◽  
Apical Region ◽  
Ciliated Cells ◽  
Transmission Electron ◽  
Herbivorous Species ◽  
Small Clusters ◽  
The Relationship ◽  
Apical Vacuole

AbstractCephalaspideans are a group of opisthobranch gastropods comprising carnivorous and herbivorous species, allowing an investigation of the relationship between these diets and the morphofunctional features of the salivary glands.In this study, the salivary glands of the carnivorous cephalaspidean Philinopsis depicta were observed by light microscopy using semithin sections and by transmission electron microscopy. A central duct runs along the length of these thin ribbon-shaped glands dividing them in two halves, each formed by a single row of tubules perpendicularly attached to the central duct. The simple epithelium of the central duct and lateral tubes contains ciliated cells and two types of secretory cells, named granular cells and cells with apical vacuole (Fig. 1). A very thin outer layer of connective tissue covers the epithelium (Fig. 1). The ciliated cells are numerous but very thin, forming small clusters between secretory cells. The nucleus, several mitochondria and a few lysosomes are located in the apical region were the cells are wider. A very thin cytoplasmic stalk reaches the base of the epithelium and contains bundles of filaments in addition to some mitochondria.

Characterization of the exposed carbohydrates on the surface membrane of adult Schistosoma mansoni by analysis of lectin binding

Parasitology ◽  
1980 ◽  
Vol 81 (1) ◽  
pp. 1-15 ◽  
Author(s):  
A. J. G. Simpson ◽  
S. R. Smithers
Keyword(s):  
Schistosoma Mansoni ◽  
Adult Male ◽  
Wheat Germ ◽  
Ricinus Communis ◽  
Specific Binding ◽  
Lectin Binding ◽  
Surface Membrane ◽  
Soybean Agglutinin ◽  
Peanut Agglutinin

SUMMARYThe surface architecture of adult male Schistosoma mansoni was explored using a range of lectins with differing carbohydrate specificities. Highest specific binding was achieved with concanavalin A and the agglutinin of molecular weight 60000 from Ricinus communis; the binding of wheat germ agglutinin was mostly non-specific. Small amounts of peanut agglutinin and soybean agglutinin binding were observed and the binding of these lectins was increased by pre-treating the parasite with neuraminidase. The fucose binding protein of Lotus tetragonolobus failed to bind. These results indicate that mannose and/or glucose, galactose, N-acetyl-glucosamine, N-acetyl-galactosamine and sialic acid are exposed on the surface of the adult male schistosome.

scholarly journals Comparative Genomic Characterization of the Multimammate Mouse Mastomys coucha

2019 ◽  
Vol 36 (12) ◽  
pp. 2805-2812
Author(s):  
Aaron Hardin ◽  
Kimberly A Nevonen ◽  
Walter L Eckalbar ◽  
Lucia Carbone ◽  
Nadav Ahituv
Keyword(s):  
Functional Characterization ◽  
Mammary Glands ◽  
Comparative Genomic ◽  
Lassa Virus ◽  
Enhancer Activity ◽  
A Genome ◽  
Genomic Tool ◽  
Mastomys Coucha ◽  
Comparative Genomic Tool

Abstract Mastomys are the most widespread African rodent and carriers of various diseases such as the plague or Lassa virus. In addition, mastomys have rapidly gained a large number of mammary glands. Here, we generated a genome, variome, and transcriptomes for Mastomys coucha. As mastomys diverged at similar times from mouse and rat, we demonstrate their utility as a comparative genomic tool for these commonly used animal models. Furthermore, we identified over 500 mastomys accelerated regions, often residing near important mammary developmental genes or within their exons leading to protein sequence changes. Functional characterization of a noncoding mastomys accelerated region, located in the HoxD locus, showed enhancer activity in mouse developing mammary glands. Combined, our results provide genomic resources for mastomys and highlight their potential both as a comparative genomic tool and for the identification of mammary gland number determining factors.

scholarly journals Growth and Characterization of Irradiated Organoids from Mammary Glands

10.3791/59293 ◽  
2019 ◽  
Author(s):  
Benjamin C. Hacker ◽  
Javier D. Gomez ◽  
Carlos A. Silvera Batista ◽  
Marjan Rafat
Keyword(s):  
Mammary Glands

Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution

2017 ◽  
Vol 54 ◽  
pp. 51-60 ◽  
Author(s):  
Carolina S. Andreotti ◽  
Celina Baravalle ◽  
Sofía C. Sacco ◽  
Melisa Lovato ◽  
Elizabet A.L. Pereyra ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Immune Response ◽  
Mammary Glands

Histochemical and Ultrastructural Characterization of the Posterior Esophagus of Bulla striata (Mollusca, Opisthobranchia)

2010 ◽  
Vol 16 (6) ◽  
pp. 688-698 ◽  
Author(s):  
Alexandre Lobo-da-Cunha ◽  
Elsa Oliveira ◽  
Íris Ferreira ◽  
Rita Coelho ◽  
Gonçalo Calado
Keyword(s):  
Digestive System ◽  
Light And Electron Microscopy ◽  
Secretory Cells ◽  
Secretory Vesicles ◽  
Ultrastructural Characterization ◽  
Herbivorous Species ◽  
Columnar Cells ◽  
Electron Lucent ◽  
Dense Vesicles

AbstractThe posterior esophagus of Bulla striata, running from the gizzard to the stomach, was investigated with light and electron microscopy to obtain new data for a comparative analysis of the digestive system in cephalaspidean opisthobranchs. In this species, the posterior esophagus can be divided into two regions. In the first, the epithelium is formed by columnar cells with apical microvilli embedded in a cuticle. Many epithelial and subepithelial secretory cells are present in this region. In both, electron-lucent secretory vesicles containing filaments and a peripheral round mass of secretory material fill the cytoplasm. These acid mucus-secreting cells may also contain a few dense secretory vesicles. In the second part of the posterior esophagus, the cuticle is absent and the epithelium is ciliated. In this region, epithelial cells may contain larger lipid droplets and glycogen reserves. Subepithelial secretory cells are not present, and in epithelial secretory cells the number of dense vesicles increases, but most secretory cells still contain some electron-lucent vesicles. These cells secrete a mixture of proteins and acid polysaccharides and should be considered seromucous. The secretory cells of the posterior esophagus are significantly different from those previously reported in the anterior esophagus of this herbivorous species.

Characterization of glucose transporters in lactating rat mammary glands

1990 ◽  
Vol 18 (4) ◽  
pp. 618-619
Author(s):  
SALLY MARTIN ◽  
ANTHONY DAVIES ◽  
HILARY A. C. FAWCETT ◽  
RUSTOM J. MADON ◽  
DAVID J. FLINT ◽  
...  
Keyword(s):  
Glucose Transporters ◽  
Mammary Glands

scholarly journals Purification and characterization of a differentiation-specific sialoglycoprotein of lactating-guinea-pig mammary tissue

1988 ◽  
Vol 251 (2) ◽  
pp. 507-514 ◽  
Author(s):  
V G Johnson ◽  
D E Greenwalt ◽  
P J Madara ◽  
I H Mather
Keyword(s):  
Sialic Acid ◽  
Guinea Pig ◽  
T Antigen ◽  
Mammary Tissue ◽  
Secretory Cells ◽  
Apical Surface ◽  
Mammary Cells ◽  
Purification And Characterization ◽  
Fat Globule

A large acidic glycoprotein, PAS-I, was purified from the fat-globule membrane of guinea-pig milk. Threonine and serine accounted for over 30 mol% of the amino acids, and galactose, N-acetylgalactosamine, N-acetylglucosamine, mannose and sialic acid were the principal sugars detected. On a molar basis, sialic acid accounted for over 60% of the total sugar. Removal of sialic acid by treatment with neuraminidase revealed the presence of binding sites for peanut (Arachis hypogaea) agglutinin, a lectin specific for the sugar sequence beta-D-Gal-(beta 1→3)-D-GalNac (the T antigen). The distribution of PAS-I-related epitopes, defined by five monoclonal antibodies, was determined in the mammary gland and in other guinea-pig tissues. PAS-I was maximally expressed on the apical surfaces of secretory cells in lactating mammary tissue and was either absent, or present in much lower amounts, in the glands of virgin or pregnant animals. PAS-I epitopes were not detected in liver, heart, spleen, pancreas, ovary, uterus, lung or intestine, either by immunofluorescence microscopy or by immunoblotting techniques. Several of the PAS-I-specific antibodies bound to mucins of high Mr in human fat-globule membrane, and similarities and differences between PAS-I and the human mucins are discussed. PAS-I and epitopes of this glycoprotein will be useful as indicators of differentiation in mammary cells and of markers of the apical surface of these cells during lactation.

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