Plant regeneration from callus cultures of salt marsh hay, Spartina patens, and its cellular-based salt tolerance

1995 ◽  
Vol 51 (1-2) ◽  
pp. 103-113 ◽  
Author(s):  
Xianggan Li ◽  
Denise M. Seliskar ◽  
Jennifer A. Moga ◽  
John L. Gallagher
Keyword(s):  
Salt Marsh ◽  
Plant Regeneration ◽  
Salt Tolerance ◽  
Callus Cultures ◽  
Spartina Patens

Isolation, Culture and Plant Regeneration From Protoplasts of Nicotiana debneyi

1980 ◽  
Vol 7 (6) ◽  
pp. 635 ◽  
Author(s):  
WR Scowcroft ◽  
PJ Larkin
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Indole Acetic Acid ◽  
High Efficiency ◽  
Protoplast Culture ◽  
Callus Cultures ◽  
Dna Uptake ◽  
Mesophyll Protoplasts ◽  
Nicotiana Debneyi ◽  
Protoplast Division

Mesophyll protoplasts of two genetically distinct genotypes of N. debneyi were cultured with sustained division following a plating efficiency in excess of 50%. Fully fertile mature plants were regenerated from callus cultures derived from protoplasts. Shoots were induced in medium containing 1 mg/l 6-benzylaminopurine and 0.5 mg/I indole acetic acid. The repeatably high efficiency of protoplast culture was used to evaluate the quantitative effects of two drugs, kanamycin and trimethoprim, which effectively inhibited colony formation at concentrations of 100 and 50 �g/ml, respectively. An enhancer of DNA uptake, poly-L-ornithine, had virtually no effect on sustained protoplast division at a concentration of 7.5 �g/ml or less.

Sign in / Sign up

Export Citation Format

Share Document