Preparation of specifically labeled milk proteins using bovine mammary-cell cultures

1965 ◽  
Vol 104 (2) ◽  
pp. 462-469 ◽  
Author(s):  
T.D.D. Groves ◽  
B.L. Larson
Keyword(s):  
Cell Cultures ◽  
Milk Proteins ◽  
Mammary Cell

GENE ACTIVATION IN MAMMARY CELLS

1972 ◽  
Vol 71 (2_Suppla) ◽  
pp. S346-S368 ◽  
Author(s):  
Roger W. Turkington ◽  
Nobuyuki Kadohama
Keyword(s):  
Cell Differentiation ◽  
Gene Transcription ◽  
Hormonal Regulation ◽  
Gene Activation ◽  
Milk Proteins ◽  
Mouse Mammary Gland ◽  
Nuclear Proteins ◽  
Mammary Cells ◽  
Alveolar Cells ◽  
Mammary Cell

ABSTRACT Hormonal activation of gene transcription has been studied in a model system, the mouse mammary gland in organ culture. Transcriptive activity is stimulated in mammary stem cells by insulin, and in mammary alveolar cells by prolactin and insulin. Studies on the template requirement for expression of the genes for milk proteins demonstrate that DNA methylation has an obligatory dependence upon DNA synthesis, but is otherwise independent from hormonal regulation of mammary cell differentiation. Incorporation of 5-bromo-2′deoxyuridine into DNA selectively inhibits expression of the genes for specific milk proteins. Undifferentiated mammary cells activate the synthesis of specific acidic nuclear proteins when stimulated by insulin. Several of these induced acidic nuclear proteins are undetectable in unstimulated undifferentiated cells, but appear to be characteristic components of the nuclei of differentiated cells. These results indicate that mammary cell differentiation is associated with a change in acidic nuclear proteins, and they provide evidence to support the concept that acidic nuclear proteins may be involved in the regulation of gene transcription and of mammary cell differentiation.

Cultivation and properties of bovine mammary cell cultures

1961 ◽  
Vol 23 (2) ◽  
pp. 373-385 ◽  
Author(s):  
K.E. Ebner ◽  
C.R. Hoover ◽  
E.C. Hageman ◽  
B.L. Larson
Keyword(s):  
Cell Cultures ◽  
Mammary Cell

scholarly journals Regulation of mouse mammary cell differentiation by extracellular milk proteins

1991 ◽  
Vol 76 (3) ◽  
pp. 379-387 ◽  
Author(s):  
CJ Wilde ◽  
DR Blatchford ◽  
M Peaker
Keyword(s):  
Cell Differentiation ◽  
Milk Proteins ◽  
Mammary Cell

Horse Milk Exosomes: Isolation, Microscopic and Biochemical Analysis, and Prospects of Use

2020 ◽  
Vol 36 (5) ◽  
pp. 62-71
Author(s):  
A.E. Kuleshova ◽  
L.V. Purvinsh ◽  
E.E. Burkova ◽  
A.E. Grigorieva ◽  
E.G. Evtushenko ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Nucleic Acids ◽  
Cell Cultures ◽  
Human Cell ◽  
Biochemical Analysis ◽  
Gel Filtration ◽  
Milk Proteins ◽  
Russian Scientific ◽  
Human Cell Cultures ◽  
Tof Ms

Horse milk exosomes have been isolated and purified via the developed technology, and their microscopic and biochemical analyzes have been carried out. It was shown that the gel-filtration on the Ultrogel A4 resin can significantly reduce the amount of milk proteins co-isolated with exosomes. Methods for the isolation of nucleic acids from the preparations at various purification stages were proposed, and the content of nucleic acids in horse milk exosomes was analyzed. It was demonstrated that horse milk exosome preparations are not toxic to human cell cultures. The prospects of using horse milk exosomes for drug delivery into cell cultures are discussed. exosomes, horse milk, exosome isolation, nucleic acids The research carried out by S.E. Sedykh, A.E. Kuleshova and E.E. Burkova was financially supported by the Russian Scientific Foundation (project no. 18-74-10055 to S.E. Sedykh); the research by G.A. Nevinskii (MALDI TOF MS/MS analysis) was supported by the basic budget financing project no. ICBFM SB RAS # АААА-А17-117020210023-1 (to G.A. Nevinsky).

scholarly journals Effect of Cell Density on Lactose Synthesis in Bovine Mammary Cell Cultures

1975 ◽  
Vol 58 (2) ◽  
pp. 159-163 ◽  
Author(s):  
Ramkishan D. Rao ◽  
Helen M. Hegarty ◽  
B.L. Larson
Keyword(s):  
Cell Density ◽  
Cell Cultures ◽  
Mammary Cell ◽  
Lactose Synthesis

Effects of two different forage sources on mammary gland growth, mammary cell turnover and activity in early lactation dairy cows

2015 ◽  
Vol 55 (5) ◽  
pp. 630 ◽  
Author(s):  
Tong Qin ◽  
Haoyu Wang ◽  
Dengpan Bu ◽  
Haisheng Hao ◽  
Dong Wang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Mammary Gland ◽  
Dairy Cows ◽  
Milk Proteins ◽  
Secretory Activity ◽  
Early Lactation ◽  
Cell Turnover ◽  
Mammary Cell ◽  
Proliferation And Apoptosis ◽  
Dietary Treatments

The objective of this study was to evaluate the effects of two different forage sources on mammary gland growth, mammary cell turnover and activity in early lactation dairy cows. Twelve early lactation cows were randomly assigned to a CS (33.8% corn straw as sole forage) or MF diet (3.7% Chinese wildrye + 28.4% alfalfa hay + 26.5% corn silage as mixed forage). All cows were fed from Week –3 to Week 8, and mammary biopsies were taken on 16 days postpartum. Mammary cell proliferation and apoptosis were determined by immunohistology, and genes expression in mammary were detected by real-time PCR. Results showed that cell proliferation, gene expression of milk proteins and proteins involved in the synthesis of milk components did not differ between two dietary treatments (P > 0.05). However, cows fed the MF diet had a higher IGF-1 receptor (IGF-1R) expression (P = 0.02), and lower rate of cell apoptosis (P = 0.003) relative to cows fed the CS diet. Collectively, these results suggest that the mammary secretory activity probably was not affected by the dietary treatments, but high quality and mixed forages led to the increased expression of IGF-1R and a larger number of cells in mammary glands, which may be responsible for the higher milk production in early lactation dairy cows.

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