DNA flow cytometric analysis of human nasopharyngeal carcinoma in nude mice

20105 Background: To observe the effect and mechanism of gemcitabine-induced radiosensitization on CNE-1 nasopharyngeal carcinoma cell line. Methods: The cell survival curves were obtained with clonogenic assay. The cell cycle phases were measured with flow cytometric analysis. Results: No cytotoxic effect was observed in 0.1 μM and 2 μM gemcitabine alone group. The radiosensitivities were observed in the groups treated with radiation and gemcitabine exposed in different consistencies and times. The SER were 1.35,1.82; 1.55 and 2.16 respectively. The G1 cell cycle phase block was observed in the two consistency groups after exposing to gemcitabine for 24 hours. Dq were rather lower in 2 μM groups especially that exposed for 24 hours. Conclusions: The radiosensitivity was obviously observed in the groups treated with two consistencies of gemcitabine. The effect was more obviously as the consistency and the exposing time increased. The block of cell cycle from G1 to S and the decrease of sublethal damage repair might be contributed to the mechanism. No significant financial relationships to disclose.

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Phenotyping of Lymphocytes in Hetero- and Homozygous Athymic NMRI Nude Mice by Computerized Two-Colored Flow-Cytometric Analysis

Contributions to Oncology - Immunodeficient Animals: Models for Cancer Research ◽

10.1159/000425221 ◽

1996 ◽

pp. 23-27

Author(s):

W. Schröder ◽

R. Strohmeier ◽

F. Alfen ◽

M. Stegmüller ◽

Z. Abasi

Keyword(s):

Nude Mice ◽

Flow Cytometric Analysis ◽

Flow Cytometric

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Diffusion kurtosis imaging and tumour microstructure for monitoring response to radiotherapy in human nasopharyngeal carcinoma xenografts

Japanese Journal of Clinical Oncology ◽

10.1093/jjco/hyaa002 ◽

2020 ◽

Vol 50 (5) ◽

pp. 548-555

Author(s):

Xiang Zheng ◽

Yunbin Chen ◽

Dechun Zheng ◽

Youping Xiao ◽

Jiayou Chen ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Nude Mice ◽

Tumour Size ◽

Diffusion Kurtosis Imaging ◽

X Ray ◽

Human Nasopharyngeal Carcinoma ◽

Xenograft Models ◽

D Values ◽

Diffusion Kurtosis ◽

Fractional Irradiation

Abstract Objective To investigate the correlations and feasibility of diffusion kurtosis imaging (DKI) parameters and tumour histopathology after radiotherapy in human nasopharyngeal carcinoma (NPC) xenografts on nude mice. Materials and Methods Seventy-two nude mice were used for the construction of CNE-1 (radio-insensitive) and CNE-2 (radio-sensitive) NPC xenograft models, followed by fraction irradiation at different doses of X-ray. The nude mice were randomly divided into six groups in each cell line models according to the dose of X-ray they have received and with six mice in each group. DKI scan was performed after radiation. DKI parameters, tumour histopathology and AQP-1 biomarkers were detected. One-way ANOVA and Pearson’s correlation analysis were used in statistical analysis. Results In CNE-1 and CNE-2 NPC xenografts, D values were increased (P < 0.01 and P < 0.001), while K values (P < 0.01 and P < 0.001) and tumour size (P < 0.001 and P < 0.001) were reduced during fraction irradiation. Additionally, cell density (CD) and AQP-1 expressions were decreased, and necrosis ratio (NR) was increased in CNE-2 xenografts after fraction irradiation (P < 0.001). The changes in D values were negatively correlated with tumour size (r = −0.856, P < 0.001), CD (r = −0.918, P < 0.001), AQP-1 mRNA (r = −0.856, P < 0.001) and protein (r = −0.381, P = 0.022) expressions while positively correlated with NR (r = 0.908, P < 0.001) in CNE-2 xenografts. The changes in K values were positively correlated with tumour size (r = 0.964, P < 0.001), CD (r = 0.888, P < 0.001), AQP-1 mRNA (r = 0.955, P < 0.001) and protein (r = 0.330, P = 0.049) expression levels while negatively correlated with NR (r = −0.930, P < 0.001). However, in CNE-1 xenografts, there were no correlation between DKI parameters and the expression of AQP-1. Conclusion Changes in D and K parameters after fractional irradiation are closely related with NPC cellular and pathological characteristics, especially size reduction and necrosis induction. These parameters exhibit potential abilities of monitoring the response to fractional irradiation in radio-sensitive NPC xenografts.

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The Flow-Cytometric Analysis of Nasopharyngeal Carcinoma Treated with Stereotactic Radiosurgery

Stereotactic and Functional Neurosurgery ◽

10.1159/000056460 ◽

2000 ◽

Vol 74 (1) ◽

pp. 30-36

Author(s):

Zhengqing Hu ◽

Renhe Dong ◽

Kangmei Huang ◽

Jiazhong Dai ◽

Li Pang

Keyword(s):

Nasopharyngeal Carcinoma ◽

Stereotactic Radiosurgery ◽

Flow Cytometric Analysis ◽

Flow Cytometric

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Suppression of human nasopharyngeal carcinoma cell growth in nude mice by the wild-type p53 gene

Journal of Cancer Research and Clinical Oncology ◽

10.1007/bf01209487 ◽

1992 ◽

Vol 119 (1) ◽

pp. 46-48 ◽

Cited By ~ 11

Author(s):

Weiping Chen ◽

Yang Lee ◽

Hui Wang ◽

Geng-Geng Yu ◽

Wei Jiao ◽

...

Keyword(s):

Cell Growth ◽

Nasopharyngeal Carcinoma ◽

Nude Mice ◽

Carcinoma Cell ◽

P53 Gene ◽

Wild Type ◽

Nasopharyngeal Carcinoma Cell ◽

Human Nasopharyngeal Carcinoma ◽

Wild Type P53

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Sequential IVIM-Parametric as a Potential Image Biomarker in Evaluating the Radiosensitivity of Human Nasopharyngeal Carcinoma Xenografts

10.21203/rs.3.rs-1050632/v1 ◽

2021 ◽

Author(s):

Youping Xiao ◽

Yunbin Chen ◽

Jianji Pan ◽

Ying Chen ◽

Xiang Zheng ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Cell Lines ◽

Nude Mice ◽

Experimental Approach ◽

Intravoxel Incoherent Motion ◽

Dynamic Changes ◽

Positive Correlation ◽

Histopathological Features ◽

Non Invasive ◽

Human Nasopharyngeal Carcinoma

Abstract BACKGROUND AND PURPOSEIntravoxel incoherent motion (IVIM) is a considerable functional MR sequence in evaluated the tumor’s response to chemo-radiotherapy. Our aim was to investigate the sequential IVIM parametric in assessing the dynamic changes of histopathological features on nasopharyngeal carcinoma (NPC) xenografts after receiving the fractional radiations.EXPERIMENTAL APPROACH: Sixty BALB/c-nu nude mice were transplanted with NPC cell lines of CNE-1 and CNE-2 to raise xenografts which further received the fractional radiations. On 3.0T MR system, IVIM (14 b-factors: 0~1000 s/mm2) was performed on xegnorafts after radiations. IVIM-parametric of D, f and D* accompanied with the cellularity and necrosis proportion of NPC xenografts were calculated and analyzed respectively.KEY RESULTSNPC xenografts exhibited a larger D and necrosis proportion coupled with a smaller D*, f and cellularity after fractional radiations, and CNE-2 xenografts presented greater changes than CNE-1 xenografts (all P<0.01). Parametric D and f correlated negatively (r=-0.824, P<0.001) while D* correlated positively with f (r=0.758, P<0.001) and D (r=0.625, P=0.042). Moreover, D correlated negatively with cellularity (rs=-0.861, P<0.001) and positively with necrosis proportion (rs=0.952, P<0.001), f behaved a positive correlation with cellularity (rs=0.627, P<0.001) and negative with necrosis proportion (rs=-0.649, P<0.001). CONCLUSIONS AND IMPLICATIONS: Sequential parametric derived from IVIM are valuable in characterizing the dynamic changes in histopathological features of NPC xenografts and can be utilized as a potential image biomarker for non-invasive assessment of tumor’s radiosensitivity.

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