Arsenic Trioxide Induced Differentiation and Apoptosis in 
      Human Nasopharyngeal Carcinoma Xenografts in BALB/C Nude Mice

Abstract Objective To investigate the correlations and feasibility of diffusion kurtosis imaging (DKI) parameters and tumour histopathology after radiotherapy in human nasopharyngeal carcinoma (NPC) xenografts on nude mice. Materials and Methods Seventy-two nude mice were used for the construction of CNE-1 (radio-insensitive) and CNE-2 (radio-sensitive) NPC xenograft models, followed by fraction irradiation at different doses of X-ray. The nude mice were randomly divided into six groups in each cell line models according to the dose of X-ray they have received and with six mice in each group. DKI scan was performed after radiation. DKI parameters, tumour histopathology and AQP-1 biomarkers were detected. One-way ANOVA and Pearson’s correlation analysis were used in statistical analysis. Results In CNE-1 and CNE-2 NPC xenografts, D values were increased (P < 0.01 and P < 0.001), while K values (P < 0.01 and P < 0.001) and tumour size (P < 0.001 and P < 0.001) were reduced during fraction irradiation. Additionally, cell density (CD) and AQP-1 expressions were decreased, and necrosis ratio (NR) was increased in CNE-2 xenografts after fraction irradiation (P < 0.001). The changes in D values were negatively correlated with tumour size (r = −0.856, P < 0.001), CD (r = −0.918, P < 0.001), AQP-1 mRNA (r = −0.856, P < 0.001) and protein (r = −0.381, P = 0.022) expressions while positively correlated with NR (r = 0.908, P < 0.001) in CNE-2 xenografts. The changes in K values were positively correlated with tumour size (r = 0.964, P < 0.001), CD (r = 0.888, P < 0.001), AQP-1 mRNA (r = 0.955, P < 0.001) and protein (r = 0.330, P = 0.049) expression levels while negatively correlated with NR (r = −0.930, P < 0.001). However, in CNE-1 xenografts, there were no correlation between DKI parameters and the expression of AQP-1. Conclusion Changes in D and K parameters after fractional irradiation are closely related with NPC cellular and pathological characteristics, especially size reduction and necrosis induction. These parameters exhibit potential abilities of monitoring the response to fractional irradiation in radio-sensitive NPC xenografts.

Download Full-text

DNA flow cytometric analysis of human nasopharyngeal carcinoma in nude mice

International Journal of Radiation Oncology*Biology*Physics ◽

10.1016/0360-3016(89)90327-1 ◽

1989 ◽

Vol 16 (2) ◽

pp. 343-345 ◽

Cited By ~ 10

Author(s):

Cao Shilong ◽

Liu Taifu ◽

Wang Zhenhua ◽

Zhou Jue ◽

Huang Kangmei ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Nude Mice ◽

Flow Cytometric Analysis ◽

Flow Cytometric ◽

Human Nasopharyngeal Carcinoma

Download Full-text

Suppression of human nasopharyngeal carcinoma cell growth in nude mice by the wild-type p53 gene

Journal of Cancer Research and Clinical Oncology ◽

10.1007/bf01209487 ◽

1992 ◽

Vol 119 (1) ◽

pp. 46-48 ◽

Cited By ~ 11

Author(s):

Weiping Chen ◽

Yang Lee ◽

Hui Wang ◽

Geng-Geng Yu ◽

Wei Jiao ◽

...

Keyword(s):

Cell Growth ◽

Nasopharyngeal Carcinoma ◽

Nude Mice ◽

Carcinoma Cell ◽

P53 Gene ◽

Wild Type ◽

Nasopharyngeal Carcinoma Cell ◽

Human Nasopharyngeal Carcinoma ◽

Wild Type P53

Download Full-text

Sequential IVIM-Parametric as a Potential Image Biomarker in Evaluating the Radiosensitivity of Human Nasopharyngeal Carcinoma Xenografts

10.21203/rs.3.rs-1050632/v1 ◽

2021 ◽

Author(s):

Youping Xiao ◽

Yunbin Chen ◽

Jianji Pan ◽

Ying Chen ◽

Xiang Zheng ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Cell Lines ◽

Nude Mice ◽

Experimental Approach ◽

Intravoxel Incoherent Motion ◽

Dynamic Changes ◽

Positive Correlation ◽

Histopathological Features ◽

Non Invasive ◽

Human Nasopharyngeal Carcinoma

Abstract BACKGROUND AND PURPOSEIntravoxel incoherent motion (IVIM) is a considerable functional MR sequence in evaluated the tumor’s response to chemo-radiotherapy. Our aim was to investigate the sequential IVIM parametric in assessing the dynamic changes of histopathological features on nasopharyngeal carcinoma (NPC) xenografts after receiving the fractional radiations.EXPERIMENTAL APPROACH: Sixty BALB/c-nu nude mice were transplanted with NPC cell lines of CNE-1 and CNE-2 to raise xenografts which further received the fractional radiations. On 3.0T MR system, IVIM (14 b-factors: 0~1000 s/mm2) was performed on xegnorafts after radiations. IVIM-parametric of D, f and D* accompanied with the cellularity and necrosis proportion of NPC xenografts were calculated and analyzed respectively.KEY RESULTSNPC xenografts exhibited a larger D and necrosis proportion coupled with a smaller D*, f and cellularity after fractional radiations, and CNE-2 xenografts presented greater changes than CNE-1 xenografts (all P<0.01). Parametric D and f correlated negatively (r=-0.824, P<0.001) while D* correlated positively with f (r=0.758, P<0.001) and D (r=0.625, P=0.042). Moreover, D correlated negatively with cellularity (rs=-0.861, P<0.001) and positively with necrosis proportion (rs=0.952, P<0.001), f behaved a positive correlation with cellularity (rs=0.627, P<0.001) and negative with necrosis proportion (rs=-0.649, P<0.001). CONCLUSIONS AND IMPLICATIONS: Sequential parametric derived from IVIM are valuable in characterizing the dynamic changes in histopathological features of NPC xenografts and can be utilized as a potential image biomarker for non-invasive assessment of tumor’s radiosensitivity.

Download Full-text

Inhibitory effects of myocardial cells culture medium on growth of human nasopharyngeal carcinoma and its mechanism in nude mice

Cell Biology International ◽

10.1042/cbi034s053d ◽

2010 ◽

Vol 34 (8) ◽

pp. S53-S53

Author(s):

Jing‑Bo Wu ◽

Yu Zheng ◽

Ling‑lin Yang ◽

Qing‑Lian Wen ◽

Zhou Su ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Nude Mice ◽

Culture Medium ◽

Myocardial Cells ◽

Inhibitory Effects ◽

Human Nasopharyngeal Carcinoma

Download Full-text

Inhibition of metastasis to lung of a human nasopharyngeal carcinoma cell line CNE- 2L2 transfected with pRc/ CMV-antisense 6A8 cDNA in nude mice

Science in China Series C Life Sciences ◽

10.1007/bf02880058 ◽

1999 ◽

Vol 42 (2) ◽

pp. 209-215

Author(s):

Lixin Zhang ◽

Yuqin Liu ◽

Fengrong Ma ◽

Pei Gu ◽

Gengxian Shi ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Cell Line ◽

Nude Mice ◽

Carcinoma Cell ◽

Carcinoma Cell Line ◽

Nasopharyngeal Carcinoma Cell ◽

Human Nasopharyngeal Carcinoma

Download Full-text

Effects of different combined regimens of cisplatin, metformin, and quercetin on nasopharyngeal carcinoma cells and subcutaneous xenografts

Scientific Reports ◽

10.1038/s41598-020-80198-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Zhongwei Chen ◽

Zhen Zeng ◽

Shanshan Zhu ◽

Ying Zeng ◽

Qihuang Lin ◽

...

Keyword(s):

Nasopharyngeal Carcinoma ◽

Nude Mice ◽

Drug Combination ◽

Drug Application ◽

Inhibitory Effects ◽

Single Drug ◽

Combined Application ◽

Subcutaneous Xenograft ◽

Recurrent Nasopharyngeal Carcinoma ◽

Combination Regimens

AbstractCisplatin, metformin, and quercetin are all reliable anticancer drugs. However, it is unclear how effective their different combination regimens are on the growth of nasopharyngeal carcinoma cell line Sune-1 and subcutaneous xenograft in nude mice. This study evaluated the effects of single-drug, two-drug, and three-drug simultaneous or sequential combined application of these drugs on the growth of Sune-1 cells and subcutaneous xenograft tumors in nude mice. The results showed that the different combination regimens of cisplatin, metformin and quercetin all had significant inhibitory effects on the proliferation of Sune-1 cells and the growth of subcutaneous xenografts in nude mice (P < 0.01), and the inhibition rate of the three drugs simultaneous combined application was significant Higher than the two-drug combination or single-drug application (P < 0.05), the contribution level of each drug in the three-drug combination application from high to low were cisplatin > metformin > quercetin. In summary, our results indicate that the simultaneous combination of cisplatin, metformin, and quercetin may synergistically inhibit the growth of Sune-1 cells and subcutaneous xenografts in nude mice through their different anticancer mechanisms, which may be clinically refractory and provide reference for chemotherapy of patients with recurrent nasopharyngeal carcinoma.

Download Full-text