scholarly journals Sequential IVIM-Parametric as a Potential Image Biomarker in Evaluating the Radiosensitivity of Human Nasopharyngeal Carcinoma Xenografts

2021 ◽  
Author(s):  
Youping Xiao ◽  
Yunbin Chen ◽  
Jianji Pan ◽  
Ying Chen ◽  
Xiang Zheng ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Cell Lines ◽  
Nude Mice ◽  
Experimental Approach ◽  
Intravoxel Incoherent Motion ◽  
Dynamic Changes ◽  
Positive Correlation ◽  
Histopathological Features ◽  
Non Invasive ◽  
Human Nasopharyngeal Carcinoma

Abstract BACKGROUND AND PURPOSEIntravoxel incoherent motion (IVIM) is a considerable functional MR sequence in evaluated the tumor’s response to chemo-radiotherapy. Our aim was to investigate the sequential IVIM parametric in assessing the dynamic changes of histopathological features on nasopharyngeal carcinoma (NPC) xenografts after receiving the fractional radiations.EXPERIMENTAL APPROACH: Sixty BALB/c-nu nude mice were transplanted with NPC cell lines of CNE-1 and CNE-2 to raise xenografts which further received the fractional radiations. On 3.0T MR system, IVIM (14 b-factors: 0~1000 s/mm2) was performed on xegnorafts after radiations. IVIM-parametric of D, f and D* accompanied with the cellularity and necrosis proportion of NPC xenografts were calculated and analyzed respectively.KEY RESULTSNPC xenografts exhibited a larger D and necrosis proportion coupled with a smaller D*, f and cellularity after fractional radiations, and CNE-2 xenografts presented greater changes than CNE-1 xenografts (all P<0.01). Parametric D and f correlated negatively (r=-0.824, P<0.001) while D* correlated positively with f (r=0.758, P<0.001) and D (r=0.625, P=0.042). Moreover, D correlated negatively with cellularity (rs=-0.861, P<0.001) and positively with necrosis proportion (rs=0.952, P<0.001), f behaved a positive correlation with cellularity (rs=0.627, P<0.001) and negative with necrosis proportion (rs=-0.649, P<0.001). CONCLUSIONS AND IMPLICATIONS: Sequential parametric derived from IVIM are valuable in characterizing the dynamic changes in histopathological features of NPC xenografts and can be utilized as a potential image biomarker for non-invasive assessment of tumor’s radiosensitivity.

Inhibiting ERp29 expression enhances radiosensitivity in human nasopharyngeal carcinoma cell lines

2011 ◽  
Vol 29 (2) ◽  
pp. 721-728 ◽  
Author(s):  
Lin Qi ◽  
Ping Wu ◽  
Xin Zhang ◽  
Yuanzheng Qiu ◽  
Weihong Jiang ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Nasopharyngeal Carcinoma Cell ◽  
Carcinoma Cell Lines ◽  
Human Nasopharyngeal Carcinoma

Diffusion kurtosis imaging and tumour microstructure for monitoring response to radiotherapy in human nasopharyngeal carcinoma xenografts

2020 ◽  
Vol 50 (5) ◽  
pp. 548-555
Author(s):  
Xiang Zheng ◽  
Yunbin Chen ◽  
Dechun Zheng ◽  
Youping Xiao ◽  
Jiayou Chen ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Nude Mice ◽  
Tumour Size ◽  
Diffusion Kurtosis Imaging ◽  
X Ray ◽  
Human Nasopharyngeal Carcinoma ◽  
Xenograft Models ◽  
D Values ◽  
Diffusion Kurtosis ◽  
Fractional Irradiation

Abstract Objective To investigate the correlations and feasibility of diffusion kurtosis imaging (DKI) parameters and tumour histopathology after radiotherapy in human nasopharyngeal carcinoma (NPC) xenografts on nude mice. Materials and Methods Seventy-two nude mice were used for the construction of CNE-1 (radio-insensitive) and CNE-2 (radio-sensitive) NPC xenograft models, followed by fraction irradiation at different doses of X-ray. The nude mice were randomly divided into six groups in each cell line models according to the dose of X-ray they have received and with six mice in each group. DKI scan was performed after radiation. DKI parameters, tumour histopathology and AQP-1 biomarkers were detected. One-way ANOVA and Pearson’s correlation analysis were used in statistical analysis. Results In CNE-1 and CNE-2 NPC xenografts, D values were increased (P &lt; 0.01 and P &lt; 0.001), while K values (P &lt; 0.01 and P &lt; 0.001) and tumour size (P &lt; 0.001 and P &lt; 0.001) were reduced during fraction irradiation. Additionally, cell density (CD) and AQP-1 expressions were decreased, and necrosis ratio (NR) was increased in CNE-2 xenografts after fraction irradiation (P &lt; 0.001). The changes in D values were negatively correlated with tumour size (r = −0.856, P &lt; 0.001), CD (r = −0.918, P &lt; 0.001), AQP-1 mRNA (r = −0.856, P &lt; 0.001) and protein (r = −0.381, P = 0.022) expressions while positively correlated with NR (r = 0.908, P &lt; 0.001) in CNE-2 xenografts. The changes in K values were positively correlated with tumour size (r = 0.964, P &lt; 0.001), CD (r = 0.888, P &lt; 0.001), AQP-1 mRNA (r = 0.955, P &lt; 0.001) and protein (r = 0.330, P = 0.049) expression levels while negatively correlated with NR (r = −0.930, P &lt; 0.001). However, in CNE-1 xenografts, there were no correlation between DKI parameters and the expression of AQP-1. Conclusion Changes in D and K parameters after fractional irradiation are closely related with NPC cellular and pathological characteristics, especially size reduction and necrosis induction. These parameters exhibit potential abilities of monitoring the response to fractional irradiation in radio-sensitive NPC xenografts.

DNA flow cytometric analysis of human nasopharyngeal carcinoma in nude mice

1989 ◽  
Vol 16 (2) ◽  
pp. 343-345 ◽  
Author(s):  
Cao Shilong ◽  
Liu Taifu ◽  
Wang Zhenhua ◽  
Zhou Jue ◽  
Huang Kangmei ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Nude Mice ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Human Nasopharyngeal Carcinoma
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