This paper originates from an address at the 8th International Symposium on
Nitrogen Fixation with Non-Legumes, Sydney, NSW, December 2000
We examined growth responses of rice seedlings
(Oryza sativaL. cv. Pelde) to specific
Rhizobium strains and their mutants, to investigate the
molecular basis of colonization and the stimulation or inhibition of rice
growth and development by rhizobia. Inoculation experiments with rice
seedlings showed that specific Rhizobium isolates of
these rice-associated and legume-associated rhizobia could either promote,
inhibit, or have no influence on rice plant growth. There are genes on certain
plasmids of Rhizobium leguminosarum bv.
trifolii and R. leguminosarum bv.
viciae that affect the growth and development of rice
root morphology. Additionally, we found that bacteria can intimately associate
with, and enter into, rice seedling roots by alternative mechanisms to those
encoded by the symbiotic (pSym) and the tumour-inducing (Ti) plasmids.
Investigations suggest an involvement of the phytohormone auxin, and possibly
nitrate, in this complex rice–Rhizobium
interaction.