Effects of sinefungin and 5′-deoxy-5′-S-isobutyl-adenosine on lipopolysaccharide-induced proliferation and protein N-methylation of arginyl residues in murine splenic lymphocytes

1991 ◽  
Vol 59 (1-3) ◽  
pp. 109-116 ◽  
Author(s):  
Bok Ryang Kim ◽  
Kyu Hwan Yang
Keyword(s):  
Splenic Lymphocytes ◽  
Arginyl Residues

The Effect of Partial Splenectomy on Platelet Production in Mice

1981 ◽  
Vol 46 (03) ◽  
pp. 602-603 ◽  
Author(s):  
H Bessler ◽  
I Notti ◽  
M Djaldetti
Keyword(s):  
Peripheral Blood ◽  
Blood Platelets ◽  
Operative Procedures ◽  
Partial Splenectomy ◽  
Splenic Lymphocytes ◽  
Platelet Production ◽  
Platelet Number ◽  
Spleen Lymphocytes

SummaryThe effect of total and partial splenectomy on the number and production of circulating platelets was studied in mice. Five days after total and partial splenectomy the number of the peripheral blood platelets increased by 87% and 60%, respectively and the incorporation of 75selenium methionine (75Se-Met) into platelets was enhanced indicating that the thrombocytosis was due to increased platelet production. The results obtained by the two operative procedures were compared.Since previous work from our laboratory has shown that a factor produced by splenic lymphocytes affects the platelet number in mice, it is suggested that the differences in the number of circulating platelets observed in animals after total and partial splenectomy may reflect a difference in the number of spleen lymphocytes removed.

scholarly journals p-Azidophenylglyoxal. A heterobifunctional photoactivable cross-linking reagent selective for arginyl residues.

1981 ◽  
Vol 256 (21) ◽  
pp. 11313-11318
Author(s):  
T.T. Ngo ◽  
C.F. Yam ◽  
H.M. Lenhoff ◽  
J. Ivy
Keyword(s):  
Cross Linking ◽  
Arginyl Residues

scholarly journals Immunologic abnormalities in an animal model of chronic hypoplastic marrow failure induced by busulfan

Blood ◽  
1978 ◽  
Vol 51 (4) ◽  
pp. 601-610 ◽  
Author(s):  
CA Pugsley ◽  
IJ Forbes ◽  
AA Morley
Keyword(s):  
B Lymphocytes ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Cell Injury ◽  
Cell Types ◽  
Peripheral Blood Lymphocytes ◽  
Delayed Type Hypersensitivity ◽  
Splenic Lymphocytes ◽  
Hypoplastic Marrow ◽  
Experimental Murine Model

Abstract The immunology of chronic hypoplastic marrow failure (CHMF, aplastic anemia) was studied in an experimental murine model of the disease induced by busulfan. B lymphocytes of peripheral blood, spleen, and bone marrow were reduced to 30%–40% and T lymphocytes of thymus, spleen, marrow, and blood were decreased to 20%–70% of control values. IgG and IgM antibody titer to sheep red blood cells were reduced to one- third of control levels, and splenic IgG, but not IgM, plaque-forming cells were fewer on day 7 after antigen stimulation. The proliferative responses to phytohemagglutinin or concanavalin A were reduced in cultures of peripheral blood lymphocytes, splenic lymphocytes, and thymocytes, and cutaneous delayed-type hypersensitivity induced by dinitrofluorobenze was not detected in mice with CHMF. The results demonstrate disturbance of a variety of cellular and humoral functions and suggest that the disturbance was due to quantitative and possibly qualitative abnormalities of the cell types subserving these functions. The results suggest that residual cell injury, the lesion underlying experimental CHMF, is not confined to the myeloid stem cell but also involved cells of the lymphoid series.

scholarly journals CELL SURFACE IMMUNOGLOBULIN

1972 ◽  
Vol 135 (6) ◽  
pp. 1392-1405 ◽  
Author(s):  
Charles J. Sherr ◽  
Sonia Baur ◽  
Inge Grundke ◽  
Joseph Zeligs ◽  
Barbara Zeligs ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Plasma Membrane ◽  
Cell Surface ◽  
Noncovalent Interactions ◽  
Subcellular Fractionation ◽  
Splenic Lymphocytes ◽  
Surface Immunoglobulin ◽  
Established Line ◽  
Intracellular Proteins ◽  
Daudi Cells

Cells from an established line of Burkitt lymphoma (Daudi) were enzymatically radioiodinated, and labeled Ig from the cell surface was isolated and studied. Subcellular fractionation of labeled cells confirmed that intracellular proteins from the cytoplasm are not iodinated by this method. Radioactive Ig was identified as monomeric (8S) IgM, and an average of 105 Ig molecules was found per cell. Ig molecules could be released from the plasma membrane by detergent lysis under nonreducing conditions indicating that attachment of Ig to the plasma membrane occurs via noncovalent interactions. The ratio of µ/L radioactivity in surface Ig was the same as that of total cellular Ig radioiodinated in solution suggesting that a large portion of the Fc fragment is not buried within the membrane. In contrast to the results obtained with cell surface Ig, most intracellular Ig was found as "free" µ- and L chains regardless of whether lysates were labeled with 125I or cells were labeled with leucine-3H. The results indicate that only a small percentage of the total Ig of Daudi cells is associated with the cell surface and suggest that covalent assembly of Ig occurs at or near the time that the molecule becomes part of the plasma membrane. Similarities between cell surface Ig on normal splenic lymphocytes and Daudi cells suggest that the latter is a neoplasm of bone marrow-derived lymphocytes.

SPLENIC LYMPHOCYTES IN CHRONIC GRANULOCYTIC LEUKÆMIA

The Lancet ◽  
1975 ◽  
Vol 306 (7923) ◽  
pp. 40
Author(s):  
Juginder Kaur ◽  
A.S.D. Spiers ◽  
D.A.G. Galton
Keyword(s):  
Splenic Lymphocytes ◽  
Chronic Granulocytic Leukaemia
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