Effect of aging on lipid peroxide levels induced by L-glutamic acid and estimated by means of a thiobarbituric acid test in rat brain tissue

Erythrocytes from rats on tocopherol-deficient and tocopherol-supplemented diets gave similar low values for lipid peroxides by the thiobarbituric acid test. Treatment of the cells from tocopherol-deficient rats with dialuric acid caused a marked increase in lipid peroxides and this closely paralleled the degree of hemolysis. Shaking of the red cells from tocopherol-deficient animals in an atmosphere of oxygen also resulted in a slow lipid peroxidation which again paralleled the degree of hemolysis. Addition of α-tocopherol to a suspension of erythrocytes from tocopherol-deficient rats decreased or completely prevented (depending upon concentration) hemolysis by dialuric acid and also decreased lipid peroxide formation.It is concluded that dialuric acid probably acts by catalyzing the formation of lipid peroxides in the unsaturated lipids of the membrane of erythrocytes from tocopherol-deficient animals. Tocopherol presumably inhibits peroxide formation, and therefore hemolysis, by virtue of its antioxidant action.

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Fluorimetric Assay for the Determination of Glutamic Acid Decarboxylase Activity in Subregions of Rat Brain Tissue

Analytical Letters ◽

10.1080/00032718008081370 ◽

1980 ◽

Vol 13 (15) ◽

pp. 1333-1344 ◽

Cited By ~ 6

Author(s):

Mack R. Holdiness ◽

Joseph B. Justice ◽

Darryl B. Neill ◽

John D. Salamone

Keyword(s):

Glutamic Acid ◽

Rat Brain ◽

Brain Tissue ◽

Glutamic Acid Decarboxylase ◽

Acid Decarboxylase ◽

Decarboxylase Activity ◽

Fluorimetric Assay ◽

Rat Brain Tissue

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Lipid Peroxidation in Rat Brain Cortical Slices as Measured by the Thiobarbituric Acid Test

Journal of Neurochemistry ◽

10.1111/j.1471-4159.1980.tb09022.x ◽

1980 ◽

Vol 35 (6) ◽

pp. 1449-1452 ◽

Cited By ~ 74

Author(s):

G. B. Kovachich ◽

O. P. Mishra

Keyword(s):

Lipid Peroxidation ◽

Rat Brain ◽

Thiobarbituric Acid ◽

Thiobarbituric Acid Test ◽

Acid Test ◽

Cortical Slices

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THE PROTECTIVE ACTION OF TOCOPHEROL AGAINST HEMOLYSIS OF RAT ERYTHROCYTES BY DIALURIC ACID

Canadian Journal of Biochemistry and Physiology ◽

10.1139/y60-119 ◽

1960 ◽

Vol 38 (1) ◽

pp. 957-964 ◽

Cited By ~ 4

Author(s):

C. C. Tsen ◽

H. B. Collier

Keyword(s):

Protective Action ◽

Lipid Peroxide ◽

Lipid Peroxides ◽

Thiobarbituric Acid ◽

Peroxide Formation ◽

Rat Erythrocytes ◽

Thiobarbituric Acid Test ◽

Unsaturated Lipids ◽

Acid Test ◽

Test Treatment

Erythrocytes from rats on tocopherol-deficient and tocopherol-supplemented diets gave similar low values for lipid peroxides by the thiobarbituric acid test. Treatment of the cells from tocopherol-deficient rats with dialuric acid caused a marked increase in lipid peroxides and this closely paralleled the degree of hemolysis. Shaking of the red cells from tocopherol-deficient animals in an atmosphere of oxygen also resulted in a slow lipid peroxidation which again paralleled the degree of hemolysis. Addition of α-tocopherol to a suspension of erythrocytes from tocopherol-deficient rats decreased or completely prevented (depending upon concentration) hemolysis by dialuric acid and also decreased lipid peroxide formation.It is concluded that dialuric acid probably acts by catalyzing the formation of lipid peroxides in the unsaturated lipids of the membrane of erythrocytes from tocopherol-deficient animals. Tocopherol presumably inhibits peroxide formation, and therefore hemolysis, by virtue of its antioxidant action.

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Liquid Chromatography Analysis of Clozapine in Rat Brain Tissue, Using its Molecularly Imprinted Polymer after Administration of Toxic Dose of Drug and Lipid Emulsion Therapy

Current Pharmaceutical Analysis ◽

10.2174/1573412914666180111160741 ◽

2019 ◽

Vol 15 (3) ◽

pp. 251-257

Author(s):

Bahareh Sadat Yousefsani ◽

Seyed Ahmad Mohajeri ◽

Mohammad Moshiri ◽

Hossein Hosseinzadeh

Keyword(s):

Rat Brain ◽

Brain Tissue ◽

Molecularly Imprinted Polymer ◽

Lipid Emulsion ◽

Limit Of Detection ◽

Imprinted Polymer ◽

Toxic Dose ◽

Molecularly Imprinted ◽

The Brain ◽

Rat Brain Tissue

Background:Molecularly imprinted polymers (MIPs) are synthetic polymers that have a selective site for a given analyte, or a group of structurally related compounds, that make them ideal polymers to be used in separation processes.Objective:An optimized molecularly imprinted polymer was selected and applied for selective extraction and analysis of clozapine in rat brain tissue.Methods:A molecularly imprinted solid-phase extraction (MISPE) method was developed for preconcentration and cleanup of clozapine in rat brain samples before HPLC-UV analysis. The extraction and analytical process was calibrated in the range of 0.025-100 ppm. Clozapine recovery in this MISPE process was calculated between 99.40 and 102.96%. The limit of detection (LOD) and the limit of quantification (LOQ) of the assay were 0.003 and 0.025 ppm, respectively. Intra-day precision values for clozapine concentrations of 0.125 and 0.025 ppm were 5.30 and 3.55%, whereas inter-day precision values of these concentrations were 9.23 and 6.15%, respectively. In this study, the effect of lipid emulsion infusion in reducing the brain concentration of drug was also evaluated.Results:The data indicated that calibrated method was successfully applied for the analysis of clozapine in the real rat brain samples after administration of a toxic dose to animal. Finally, the efficacy of lipid emulsion therapy in reducing the brain tissue concentration of clozapine after toxic administration of drug was determined.Conclusion:The proposed MISPE method could be applied in the extraction and preconcentration before HPLC-UV analysis of clozapine in rat brain tissue.

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