5091304 Whole blood activated partial thromboplastin time test and associated apparatus

1992 ◽  
Vol 10 (2) ◽  
pp. 323
Keyword(s):  
Whole Blood ◽  
Partial Thromboplastin Time ◽  
Activated Partial Thromboplastin Time ◽  
Time Test

Monitoring Anticoagulant Therapy by Activated Partial Thromboplastin Time: Hirudin Assessment

1994 ◽  
Vol 72 (05) ◽  
pp. 685-692 ◽  
Author(s):  
Michael T Nurmohamed ◽  
René J Berckmans ◽  
Willy M Morriën-Salomons ◽  
Fenny Berends ◽  
Daan W Hommes ◽  
...  
Keyword(s):  
Whole Blood ◽  
Partial Thromboplastin Time ◽  
Ex Vivo ◽  
Fold Increase ◽  
Heparin Therapy ◽  
Activated Partial Thromboplastin Time ◽  
Recombinant Hirudin ◽  
Interindividual Differences ◽  
The Relationship

SummaryBackground. Recombinant hirudin (RH) is a new anticoagulant for prophylaxis and treatment of venous and arterial thrombosis. To which extent the activated partial thromboplastin time (APTT) is suitable for monitoring of RH has not been properly evaluated. Recently, a capillary whole blood device was developed for bed-side monitoring of the APTT and it was demonstrated that this device was suitable to monitor heparin therapy. However, monitoring of RH was not evaluated.Study Objectives. To evaluate in vitro and ex vivo the responsiveness and reproducibility for hirudin monitoring of the whole blood monitor and of plasma APTT assays, which were performed with several reagents and two conventional coagulometers.Results. Large interindividual differences in hirudin responsiveness were noted in both the in vitro and the ex vivo experiments. The relationship between the APTT, expressed as clotting time or ratio of initial and prolonged APTT, and the hirudin concentration was nonlinear. A 1.5-fold increase of the clotting times was obtained at 150-200 ng/ml plasma. However, only a 2-fold increase was obtained at hirudin levels varying from 300 ng to more than 750 ng RH/ml plasma regardless of the assays. The relationship linearized upon logarithmic conversion of the ratio and the hirudin concentration. Disregarding the interindividual differences, and presuming full linearity of the relationship, all combinations were equally responsive to hirudin.Conclusions. All assays were equally responsive to hirudin. Levels up to 300 ng/ml plasma can be reliably estimated with each assay. The manual device may be preferable in situations where rapid availability of test results is necessary.

Time test

1998 ◽  
Vol 79 (3) ◽  
pp. 227-231
Author(s):  
L. N. Tarasova ◽  
E. Yu. Savinykh ◽  
G. K. Platonov ◽  
L. L.N. Tonin ◽  
O. I. Rechkin
Keyword(s):  
Partial Thromboplastin Time ◽  
Raw Materials ◽  
Test Tube ◽  
Activated Partial Thromboplastin Time ◽  
Therapy Control ◽  
The World ◽  
Time Test

The data on standardization of the activated partial thromboplastin time test all over the world and in Russia are given. The method is used as a screening one and it is of importance for revealing disorders in the first coagulation phase (hemophilia diagnosis) and heparinotherapy control. Two lyophilized forms of partial thromboplastin made of cadaverine raw materials are developed. Their specificity in revealing hemophilia, therapy control by heparin fit for a year is confirmed. The diagnosticum of one of them is a basis of the kit for determining the activated partial thromboplastin time. The possibility of its use not only in performing the test by test tube methods but as well by semiautomatic and automatic machines.

Haemorrhage in seven cats with suspected anticoagulant rodenticide intoxication

2003 ◽  
Vol 5 (5) ◽  
pp. 295-304 ◽  
Author(s):  
B Kohn ◽  
C Weingart ◽  
U Giger
Keyword(s):  
Body Weight ◽  
Blood Plasma ◽  
Vitamin K ◽  
Whole Blood ◽  
Partial Thromboplastin Time ◽  
Reference Range ◽  
Activated Partial Thromboplastin Time ◽  
Anticoagulant Rodenticide ◽  
Plasma Coagulation ◽  
Adult Cats

Clinical features were evaluated in seven adult cats (six males, one female) with haemorrhage and presumptive anticoagulant rodenticide intoxication. Haemorrhage appeared as thoracic haemorrhage, otic bleeding, haematoma, melena, haematochezia, and petechiation. The most common other presenting signs were lethargy, anorexia, and tachypnoea or dyspnoea. Six cats were anaemic, four cats were mildly thrombocytopenic (58 000–161 000/μl), and three had slightly decreased plasma protein or albumin values. The prothrombin time (30.3–>100 s, reference range: 16.5–27.5 s) and activated partial thromboplastin time values (32.6–>100 s; reference range: 14–25 s) were markedly prolonged in all cats. All cats received vitamin K1 subcutaneously or orally (3.7–5 mg/kg body weight initially) and depending on severity of signs five cats were transfused with fresh whole blood. Plasma coagulation times improved in all cats and returned to normal in 1–5 days. Rodenticide poisons represent an important but relatively rare cause of haemorrhage in cats and can be effectively treated.

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