Induction of a humoral immune response to a Shiga toxin B subunit epitope expressed as a chimeric LamB protein in a Shigella flexneri live vaccine strain

1992 ◽  
Vol 12 (6) ◽  
pp. 399-407 ◽  
Author(s):  
Marie Ryd ◽  
Naresh Verma ◽  
Alf A. Lindberg
Keyword(s):  
Immune Response ◽  
Vaccine Strain ◽  
Shiga Toxin ◽  
Humoral Immune Response ◽  
Shigella Flexneri ◽  
Live Vaccine ◽  
Live Vaccine Strain ◽  
Toxin B ◽  
Humoral Immune ◽  
B Subunit

Expression and characterization of cholera toxin B—pneumococcal surface adhesin A fusion protein in Escherichia coli: ability of CTB-PsaA to induce humoral immune response in mice

2004 ◽  
Vol 321 (1) ◽  
pp. 192-196 ◽  
Author(s):  
Ana Paula Mattos Arêas ◽  
Maria Leonor Sarno Oliveira ◽  
Eliane Namie Miyaji ◽  
Luciana Cezar Cerqueira Leite ◽  
Karina Araújo Aires ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Immune Response ◽  
Fusion Protein ◽  
Cholera Toxin ◽  
Humoral Immune Response ◽  
Toxin B ◽  
Cholera Toxin B ◽  
Humoral Immune

B subunit ofEscherichia coliheat-labile enterotoxin as adjuvant of humoral immune response in recombinant BCG vaccination

2008 ◽  
Vol 54 (8) ◽  
pp. 677-686 ◽  
Author(s):  
Andréa da Silva Ramos Rocha ◽  
Fabricio Rochedo Conceição ◽  
André Alex Grassmann ◽  
Valeska Lizzi Lagranha ◽  
Odir Antônio Dellagostin
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Biological Properties ◽  
Adjuvant Effect ◽  
Strong Immune Response ◽  
Humoral Immune ◽  
B Subunit ◽  
Th2 Immune Response ◽  
Antibody Levels ◽  
Recombinant Bcg

The B subunit of Escherichia coli heat-labile enterotoxin (LTB), a nontoxic molecule with potent biological properties, is a powerful mucosal and parenteral adjuvant that induces a strong immune response against co-administered or coupled antigens. In this paper, the effect of LTB on the humoral immune response to recombinant BCG (rBCG) vaccination was evaluated. Isogenic mice were immunized with rBCG expressing the R1 repeat region of the P97 adhesin of Mycoplasma hyopneumoniae alone (rBCG/R1) or fused to LTB (rBCG/LTBR1). Anti-R1 systemic antibody levels (IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA) were measured by ELISA using recombinant R1 as antigen. With the exception of IgM, LTB doubled the anti-R1 antibody levels in rBCG vaccination. The IgG1/IgG2a mean ratio showed that both rBCG/LTBR1 and rBCG/R1 induced a mixed Th1/Th2 immune response. Interestingly, anti-R1 serum IgA was induced only by rBCG/LTBR1. These results demonstrate that LTB has an adjuvant effect on the humoral immune response to recombinant antigens expressed in BCG.

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