Safety evaluation of petroleum products using an in vitro eye irritation test battery

1994 ◽  
Vol 8 (4) ◽  
pp. 715-717
Author(s):  
S.A. Martin ◽  
T.A. Roy ◽  
K.A. Saladdin ◽  
B.A. Fleming ◽  
C.R. Mackerer
Keyword(s):  
Safety Evaluation ◽  
Petroleum Products ◽  
Test Battery ◽  
Eye Irritation ◽  
Irritation Test

The CTFA evaluation of alternatives program: An evaluation of in vitro alternatives to the draize primary eye irritation test. (Phase II) oil/water emulsions

1994 ◽  
Vol 32 (10) ◽  
pp. 943-976 ◽  
Author(s):  
S.D. Gettings ◽  
L.C. Dipasquale ◽  
D.M. Bagley ◽  
P.L. Casterton ◽  
M. Chudkowski ◽  
...  
Keyword(s):  
Phase Ii ◽  
Test Phase ◽  
Eye Irritation ◽  
Irritation Test ◽  
Oil Water

Me-too validation study for in vitro eye irritation test with 3D-reconstructed human cornea epithelium, MCTT HCETM

2019 ◽  
Vol 55 ◽  
pp. 173-184 ◽  
Author(s):  
Song-E Lim ◽  
Su Jin Ha ◽  
Won-Hee Jang ◽  
Kyung-Mi Jung ◽  
Mi-Sook Jung ◽  
...  
Keyword(s):  
Validation Study ◽  
Human Cornea ◽  
Eye Irritation ◽  
Irritation Test ◽  
Cornea Epithelium

scholarly journals GREEN TEA EXTRACT IN AN EYELASH GROWTH ENHANCER GEL FORMULATION: STABILITY TEST, EYE IRRITATION TEST, AND HUMAN EYELASH GROWTH ACTIVITY

2017 ◽  
Vol 10 (6) ◽  
pp. 243
Author(s):  
Indra Syahputra Roes Lie ◽  
Joshita Djajadisastra ◽  
Fadlina Chany Saputri
Keyword(s):  
Green Tea ◽  
Chorioallantoic Membrane ◽  
Test Group ◽  
Green Tea Extract ◽  
Stability Test ◽  
Eye Irritation ◽  
Before And After ◽  
Irritation Test ◽  
Tea Extract

Objective: To formulate a green tea extract (GTE), which is often used as a hair growth product, to produce an eyelash gel with good stability, effectiveness, and safety for growing eyelashes.Methods: GTE was formulated into a gel. A stability test was performed at a high temperature (40±2°C), room temperature (25±2°C), low temperature (4±2°C), and a cycling temperature. An in vitro hen’s egg test-chorioallantoic membrane assay was performed to evaluate potential eye irritation. An eyelash growth test was conducted by length measurement using an eyelash ruler before and after 2 mo of application in human volunteers. Results: The GTE gel was stable in storage at high, room, and low temperatures and at cycling temperatures and did not cause eye irritation. Eyelashes grew significantly more in the test group than in the placebo group after 2 mo of application (p<0.05). Conclusion: GTE gel provides a new, safe, and effective option for growing natural eyelashes.

Development of the short time exposure (STE) test: An in vitro eye irritation test using SIRC cells

2008 ◽  
Vol 22 (3) ◽  
pp. 760-770 ◽  
Author(s):  
Yutaka Takahashi ◽  
Mirei Koike ◽  
Hiroshi Honda ◽  
Yuichi Ito ◽  
Hitoshi Sakaguchi ◽  
...  
Keyword(s):  
Eye Irritation ◽  
Time Exposure ◽  
Irritation Test ◽  
Short Time

Evaluation of the bovine corneal opacity-permeability assay as an in vitro alternative to the Draize eye irritation test

1993 ◽  
Vol 7 (4) ◽  
pp. 471-476 ◽  
Author(s):  
Ph. Vanparys ◽  
Gh. Deknudt ◽  
M. Sysmans ◽  
G. Teuns ◽  
W. Coussement ◽  
...  
Keyword(s):  
Corneal Opacity ◽  
Eye Irritation ◽  
Irritation Test ◽  
Permeability Assay

Toxicity of Detergent-based Commercial Products on Cells of a Mouse Line in Suspension Culture as a Possible Screen for the Draize Rabbit Eye Irritation Test

1983 ◽  
Vol 11 (1) ◽  
pp. 15-21 ◽  
Author(s):  
R.B. Kemp ◽  
R.W.J. Meredith ◽  
S. Gamble ◽  
M. Frost
Keyword(s):  
In Vitro Test ◽  
Commercial Products ◽  
Eye Irritation ◽  
Rabbit Eye ◽  
Irritation Test ◽  
Vitro Test ◽  
Mild Irritants ◽  
Cells Cultured

Summary The Draize rabbit eye irritation test has several disadvantages and inadequacies when used as an indicator for potential irritancy of detergent-based commercial products. As a possible in vitro screen, it was decided to use mouse LS cells cultured in suspension, taking 50% cell death (CD50) after exposure to the product for 4h as the endpoint. This figure for 11 formulations was compared with eye irritation data ranked using an arbitrary classification of mild, moderate and severe. All samples with a CD50 less than 0.5mg/ml were severe eye irritants, while most of those with a CD50 greater than 1.0mg/ml were mild irritants. Samples between these two cytotoxicity levels were, in general, moderately irritant to the rabbit eye. It would appear that this in vitro test is a possible screen for the irritancy of detergent-based products.

Validation and Regulatory Acceptance of Alternatives

1999 ◽  
Vol 8 (1) ◽  
pp. 73-79 ◽  
Author(s):  
RICHARD N. HILL ◽  
WILLIAM S. STOKES
Keyword(s):  
Ad Hoc ◽  
Toxicity Testing ◽  
Test Method ◽  
Regulatory Agencies ◽  
Eye Irritation ◽  
Current Test ◽  
Irritation Test ◽  
Test Guideline

For years there was no focus within the U.S. federal government for alternatives to animal toxicity testing. Questions coming to regulatory agencies fell upon individuals to address in the best way they could. Given this void, the ad hoc Interagency Regulatory Alternatives Group was founded by staff in a number of federal agencies in the late 1980s to coalesce efforts in the field. The group sponsored two international workshops on eye irritation, the first making proposals for change in the current test method in rabbits, the second reviewing available data on in vitro alternatives. The result has been that the Organization for Economic Cooperation and Development (OECD) is considering revision of the in vivo eye irritation test guideline to incorporate a number of the workshop deliberations. However, movement of the in vitro eye irritation alternatives has been disappointing; attempts to determine their practical testing significance have thus far been unrewarding.

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