The role of autoreactive T-cell lines in the restoration of the immune response to sheep red blood cells in C57B16-lprlpr mice

Irradiated rabbits given allogeneic bone marrow cells from normal adult donors responded to an injection of sheep red blood cells by forming circulating antibodies. Their spleen cells were also capable of forming many plaques using the hemolysis in gel technique, and were also capable of undergoing blastogenesis and mitosis and of incorporating tritiated thymidine upon exposure to the specific antigen in vitro. However, irradiated rabbits injected with allogeneic bone marrow obtained from rabbits injected with sheep red blood cells 24 hr prior to sacrifice (primed donors) were incapable of mounting an immune response after stimulation with sheep red cells. This loss of reactivity by the bone marrow from primed donors is specific for the antigen injected, since the immune response of the irradiated recipients to a non-cross-reacting antigen, the horse red blood cell, is unimpaired. Treatment of the bone marrow donors with high-titered specific antiserum to sheep red cells for 24 hr prior to sacrifice did not result in any diminished ability of their bone marrow cells to transfer antibody-forming capacity to sheep red blood cells. The significance of these results, with respect to the origin of the antigen-reactive and antibody-forming cells in the rabbit, is discussed.

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Role of mitochondrial glucocorticoid receptor in glucocorticoid-induced apoptosis

Journal of Experimental Medicine ◽

10.1084/jem.20050433 ◽

2006 ◽

Vol 203 (1) ◽

pp. 189-201 ◽

Cited By ~ 87

Author(s):

Ronit Vogt Sionov ◽

Orly Cohen ◽

Shlomit Kfir ◽

Yael Zilberman ◽

Eitan Yefenof

Keyword(s):

T Cell ◽

Glucocorticoid Receptor ◽

Cell Lines ◽

Cell Types ◽

Thymic Epithelial Cells ◽

Dependent Manner ◽

Localization Signal ◽

Induced Apoptosis ◽

T Cell Lines

The mechanisms by which glucocorticoid receptor (GR) mediates glucocorticoid (GC)-induced apoptosis are unknown. We studied the role of mitochondrial GR in this process. Dexamethasone induces GR translocation to the mitochondria in GC-sensitive, but not in GC-resistant, T cell lines. In contrast, nuclear GR translocation occurs in all cell types. Thymic epithelial cells, which cause apoptosis of the PD1.6 T cell line in a GR-dependent manner, induce GR translocation to the mitochondria, but not to the nucleus, suggesting a role for mitochondrial GR in eliciting apoptosis. This hypothesis is corroborated by the finding that a GR variant exclusively expressed in the mitochondria elicits apoptosis of several cancer cell lines. A putative mitochondrial localization signal was defined to amino acids 558–580 of human GR, which lies within the NH2-terminal part of the ligand-binding domain. Altogether, our data show that mitochondrial and nuclear translocations of GR are differentially regulated, and that mitochondrial GR translocation correlates with susceptibility to GC-induced apoptosis.

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Acute Inflammatory Response to Sheep Red Blood Cells in Mice Treated with 2,3,7,8-Tetrachlorodibenzo-p-dioxin: The Role of Proinflammatory Cytokines, IL-1 and TNF

Toxicology and Applied Pharmacology ◽

10.1006/taap.1994.1169 ◽

1994 ◽

Vol 127 (2) ◽

pp. 331-335 ◽

Cited By ~ 27

Author(s):

A.B. Moos ◽

L. Baechersteppan ◽

N.I. Kerkvliet

Keyword(s):

Inflammatory Response ◽

Red Blood Cells ◽

Proinflammatory Cytokines ◽

Blood Cells ◽

Acute Inflammatory Response ◽

Sheep Red Blood Cells ◽

Tetrachlorodibenzo P Dioxin

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Gamma gene rearrangement and expression in autoreactive helper T cells.

Journal of Experimental Medicine ◽

10.1084/jem.163.5.1314 ◽

1986 ◽

Vol 163 (5) ◽

pp. 1314-1318 ◽

Cited By ~ 24

Author(s):

M Zauderer ◽

A Iwamoto ◽

T W Mak

Keyword(s):

T Cell ◽

Cell Lines ◽

Class Ii ◽

Cytotoxic T Cell ◽

Specific Class ◽

Helper T Cell ◽

Autoreactive T Cell ◽

Cell Clones ◽

Class Ii Mhc ◽

T Cell Lines

gamma gene rearrangements similar to those described for cytotoxic T cell lines are found in L3T4+, autoreactive, or KLH-specific cloned helper T cell lines. High levels of gamma RNA transcripts were, in addition, detected in four out of five L3T4+, class II MHC-specific, autoreactive T cell clones, and in at least one of three KLH-specific, class II MHC-restricted clones. This contrasts with previously reported (9) expression of gamma RNA in only 1 of 11 antigen-specific helper T cell lines.

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