Photoreactivity of biologically active compounds VII. Interaction of antimalarial drugs with melanin in vitro as part of phototoxicity screening

The aim of the research was to establish efficient microclonal propagation conditions of endangered Crambe mitridatis plants in vitro and to study the possible effect of aseptic cultivation on biochemical composition (hydroxycinnamic acids, flavonoids, phenolic compounds) of plants. Methods. In vitro plant culture methods were applied. Seeds were used for aseptic culture initiation. Morphogenic potential of root, leaf and petiole explants was studied on Murashige-Skoog medium with addition of plant growth regulators. The content of biologically active compounds was measured using spectrometry in plants grown in aseptic conditions and in the greenhouse. Results. Morphogenic potential of root, leaf and petiole explants was studied and the highest regeneration frequency of plantlets was established for root explants (80 %), for petiole explants (50 %) and the lowest for leaf explants (20 %). It was found that plants cultivated in aseptic conditions have higher hydroxycinnamic acids, flavonoids and phenolic compounds compared to plants grown in vivo. Conclusions. It is advisable to multiply C. mitridatis plants in vitro via root and petiole explants. Aseptic cultivation contributes to synthesis of biologically active compounds (auxin synergists) in C. mitridatis plants.Keywords: in vitro culture, hydroxycinnamic acids, flavonoids, phenolic compounds, Crambe mіtridatis.

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Broccoli isothiocyanates content and in vitro availability according to variety and origin

Macedonian Journal of Chemistry and Chemical Engineering ◽

10.20450/mjcce.2013.354 ◽

2013 ◽

Vol 32 (1) ◽

pp. 251 ◽

Cited By ~ 7

Author(s):

M. Carmen Rodríguez-Hernández ◽

Sonia Medina ◽

Angel Gil-Izquierdo ◽

Carmen Martínez-Ballesta ◽

Diego A. Moreno

Keyword(s):

Cancer Chemoprevention ◽

Biologically Active Compounds ◽

Biologically Active ◽

Active Compounds ◽

In Vitro Availability ◽

Different Sources

Broccoli is considered one of the healthiest vegetables own to its high content of beneficial biologically active compounds – the breakdown products of the glucosinolates, the isothiocyanates. The aim of this work was to characterize the production of isothiocyanates (sulforaphane and iberin and related metabolites) from different sources of glucosinolates by means of comparison between different broccoli cultivars and commercial samples, investigating their composition and in vitro bioavailability. Differences in the major intact glucosinolates were observed between the different experimental and commercial samples showing lower concentrations of glucosinolates the latest. The simulation of digestion reduced the concentration of parental phytochemicals (glucosinolates) producing the hydrolysis to biologically active isothiocyanates. The commercial samples showed lower levels of ITCs than experimental broccoli cultivars. Measurement of the glucosinolates ingested by UPLC-QqQ-MS/MS has allowed an exact quantifying of these compounds, particularly isothiocyanates, which will help to futures cancer chemoprevention studies.

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IN VITRO EFECTS OF SELECTED BIOLOGICALLY ACTIVE COMPOUNDS ON RABBIT SPERMATOZOA MOTILITY BEHAVIOUR

Journal of Microbiology Biotechnology and Food Sciences ◽

10.15414/jmbfs.2017.6.6.1290-1294 ◽

2017 ◽

Vol 6 (6) ◽

pp. 1290-1294 ◽

Cited By ~ 1

Author(s):

Michal Ďuračka ◽

Marek Halenár ◽

Eva Tvrdá

Keyword(s):

Biologically Active Compounds ◽

Biologically Active ◽

Active Compounds ◽

Spermatozoa Motility ◽

Rabbit Spermatozoa

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Modified approach for preparing (E)-stilbenes related to resveratrol, and evaluation of their potential immunobiological effects

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc2009531 ◽

2010 ◽

Vol 75 (2) ◽

pp. 175-186 ◽

Cited By ~ 11

Author(s):

Jan Šmidrkal ◽

Juraj Harmatha ◽

Miloš Buděšínský ◽

Karel Vokáč ◽

Zdeněk Zídek ◽

...

Keyword(s):

Molecular Structure ◽

Nitric Oxide ◽

Interferon Γ ◽

Biologically Active Compounds ◽

Biologically Active ◽

Active Compounds ◽

Boron Trichloride ◽

Stilbene Derivatives ◽

Boron Tribromide

Resveratrol and closely related stilbenoids belong to the most intensively studied biologically active compounds. This interest evoked several attempts to prepare such compounds in a convenient synthetic way. Our approach allowed obtaining largely methoxystilbenes, formed as E-isomers only (using Wittig–Horner synthesis as the key step), which were further demethylated by boron tribromide. The hydroxymethoxystilbenes (e.g. pterostilbene) were prepared using isopropyl protection, later selectively deprotected by boron trichloride. The method is suitable for preparing such compounds in a large amount. Effects of the obtained stilbene derivatives on immunobiological responses triggered by lipopolysacharide and interferon-γ were tested under in vitro conditions. Namely production of nitric oxide (NO) was investigated, and relation between the molecular structure and immunobiological activity was assessed.

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Downregulation of duodenal SLC transporters and activation of proinflammatory signaling constitute the early response to high altitude in humans

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00353.2013 ◽

2014 ◽

Vol 307 (7) ◽

pp. G673-G688 ◽

Cited By ~ 10

Author(s):

Kacper A. Wojtal ◽

Alexandra Cee ◽

Silvia Lang ◽

Oliver Götze ◽

Heiko Frühauf ◽

...

Keyword(s):

High Altitude ◽

Serum Levels ◽

Biologically Active Compounds ◽

Biologically Active ◽

Matrix Metalloproteinase 2 ◽

Mrna Levels ◽

Active Compounds ◽

Slc Transporters ◽

Ifn Γ

Solute carrier (SLC) transporters mediate the uptake of biologically active compounds in the intestine. Reduced oxygenation (hypoxia) is an important factor influencing intestinal homeostasis. The aim of this study was to investigate the pathophysiological consequences of hypoxia on the expression and function of SLCs in human intestine. Hypoxia was induced in human intestinal epithelial cells (IECs) in vitro (0.2; 1% O2 or CoCl2). For human in vivo studies, duodenal biopsies and serum samples were obtained from individuals ( n = 16) acutely exposed to 4,554 meters above sea levels. Expression of relevant targets was analyzed by quantitative PCR, Western blotting, or immunofluorescence. Serum levels of inflammatory mediators and nucleosides were determined by ELISA and LC/MS-MS, respectively. In the duodenum of volunteers exposed to high altitude we observed decreased mRNA levels of apical sodium-dependent bile acid transporter (ASBT), concentrative nucleoside transporters 1/2 (CNT1/2), organic anion transporting polypeptide 2B1 (OATP2B1), organic cation transporter 2 (OCTN2), peptide transporter 1 (PEPT1), serotonin transporter (SERT), and higher levels of IFN-γ, IL-6, and IL-17A. Serum levels of IL-10, IFN-γ, matrix metalloproteinase-2 (MMP-2), and serotonin were elevated, whereas the levels of uridine decreased upon exposure to hypoxia. Hypoxic IECs showed reduced levels of equilibrative nucleoside transporter 2 (ENT2), OCTN2, and SERT mRNAs in vitro, which was confirmed on the protein level and was accompanied by activation of ERK1/2, increase of hypoxia-inducible factor (HIF) proteins, and production of IL-8 mRNA. Costimulation with IFN-γ and IL-6 during hypoxia further decreased the expression of SERT, ENT2, and CNT2 in vitro. Reduced oxygen supply affects the expression pattern of duodenal SLCs that is accompanied by changes in serum levels of proinflammatory cytokines and biologically active compounds demonstrating that intestinal transport is affected during systemic exposure to hypoxia in humans.

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