scholarly journals The effect of aseptic cultivation of Crambe mitridatis Juz. plants on its biochemical composition

2020 ◽  
Vol 17 (2) ◽  
pp. 172-178
Author(s):  
N. O. Pushkarova ◽  
T. M. Kyrpa-Nesmiian ◽  
M. V. Kuchuk
Keyword(s):  
Phenolic Compounds ◽  
Biochemical Composition ◽  
Leaf Explants ◽  
Biologically Active Compounds ◽  
Hydroxycinnamic Acids ◽  
Biologically Active ◽  
Active Compounds ◽  
Petiole Explants ◽  
Aseptic Conditions

The aim of the research was to establish efficient microclonal propagation conditions of endangered Crambe mitridatis plants in vitro and to study the possible effect of aseptic cultivation on biochemical composition (hydroxycinnamic acids, flavonoids, phenolic compounds) of plants. Methods. In vitro plant culture methods were applied. Seeds were used for aseptic culture initiation. Morphogenic potential of root, leaf and petiole explants was studied on Murashige-Skoog medium with addition of plant growth regulators. The content of biologically active compounds was measured using spectrometry in plants grown in aseptic conditions and in the greenhouse. Results. Morphogenic potential of root, leaf and petiole explants was studied and the highest regeneration frequency of plantlets was established for root explants (80 %), for petiole explants (50 %) and the lowest for leaf explants (20 %). It was found that plants cultivated in aseptic conditions have higher hydroxycinnamic acids, flavonoids and phenolic compounds compared to plants grown in vivo. Conclusions. It is advisable to multiply C. mitridatis plants in vitro via root and petiole explants. Aseptic cultivation contributes to synthesis of biologically active compounds (auxin synergists) in C. mitridatis plants.Keywords: in vitro culture, hydroxycinnamic acids, flavonoids, phenolic compounds, Crambe mіtridatis.

IN VITRO INVESTIGATION OF THE PERMEABILITY OF BIOLOGICALLY ACTIVE COMPOUNDS

2016 ◽  
Vol 33 (59) ◽  
pp. 41-45
Author(s):  
Tatyana A. Grigoreva ◽  
◽  
Aleksandr V. Garabadzhiu ◽  
Vyacheslav G. Tribulovich ◽  
◽  
...  
Keyword(s):  
Biologically Active Compounds ◽  
Biologically Active ◽  
Active Compounds ◽  
In Vitro Investigation

scholarly journals Biologically active compounds from cyanobacteria extracts:in vivo and in vitro aspects

2013 ◽  
Vol 23 (3) ◽  
pp. 471-480 ◽  
Author(s):  
Luciana R. Carvalho ◽  
Adriana Costa-Neves ◽  
Geanne A.A. Conserva ◽  
Rafael L. Brunetti ◽  
Guilherme S. Hentschke ◽  
...  
Keyword(s):  
Biologically Active Compounds ◽  
Biologically Active ◽  
Active Compounds

scholarly journals Biologically Active Compounds and Antioxidant Activity of the Plants from CSBG SB RAS Collection of the Asteraceae Family

2021 ◽  
Vol 38 ◽  
pp. 00055
Author(s):  
Elena Khramova ◽  
Tatyana Kukushkina ◽  
Tatyana Shaldaeva ◽  
Yuliya Pshenichkina
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Achillea Millefolium ◽  
Artemisia Absinthium ◽  
Tanacetum Vulgare ◽  
Active Compounds ◽  
Artemisia Dracunculus ◽  
Pectin Substances

The article presents Data on the content of biologically active compounds (BAC) and the total phenolic antioxidants activity (TPA) evaluated for leaves and inflorescences of Achillea millefolium, Anthemis tinctoria, Artemisia absinthium, A. dracunculus, Leucanthemum vulgare, Pyrethrum balsamita, P. macrophyllum, Tanacetum vulgare. The leaves and inflorescences of the plants contain flavonols, catechins, tannins, carotenoids, pectin substances. High content of phenolic compounds was found in Artemisia dracunculus and Achillea millefolium (352.34 – 398.99 mg/g of DW). The highest level of catechins was registered in inflorescences (8.01 mg/g) and leaves (4.58 mg/g) of Artemisia absinthium. The content of pectin substances in the studied plants was quite high, with the biggest share of protopectins (50.6 – 95.9 mg/g). The maximum quantity of pectins was found in the leaves and inflorescences of Leucanthemum vulgare (11.2-12.7 mg/g), protopectins – in the leaves of Tanacetum vulgare (95.9 mg/g). The highest content of carotenoids was found in the leaves of plants, except for Leucanthemum vulgare. The highest antioxidant activity was recorded for water-ethanol extracts from leaves of Achillea millefolium (up to 1.09 mg/g) and inflorescences of Artemisia dracunculus (0.94 mg/g) plants due to the increased content of phenolic compounds, in particular flavonols and tannins, compared to the other species.

scholarly journals Research of biologically active compounds of catalpa (Catalpa bignonioides Walt.)

2020 ◽  
pp. 52-58
Author(s):  
O. V. Demeshko ◽  
S. V. Romanova ◽  
V. V. Kovalyov ◽  
I. I. Batiuchenko
Keyword(s):  
Acetic Acid ◽  
Phenolic Compounds ◽  
Chromatographic Analysis ◽  
Skin Diseases ◽  
Biologically Active Compounds ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Active Compounds ◽  
Chromatographic Paper ◽  
Active Substances

Catalpa bignonioides Walt. (catalpa) the family Bignoniaceae – a tree, which originates from North America. Catalpa bark contains resins and tannins. Monoterpene glycosides were found in the leaf. Catalpa seeds contain up to 30% of eleostearic acid. Decoctions are made of the bark of the tree. They serve as a gastric agent and have an anthelmintic effect. Decoction of the bark exhibits bronchodilator action, is used in the complex treatment of cancer. The leaves are used to treat skin diseases. The infusion of fruits has anti-herpetic and hypoglycemic properties. The aim of the work is to investigate biologically active compounds of the leaves of catalpa bignini. The object of the study was the leaves of common catalpa harvested in the summer of 2018 in Sevastopol. Chemical identification reactions and methods of chromatographic analysis were used to determine the major groups of biologically active substances of the leaves of the catalpa (chromatographic paper Filtrak FN No. 4, Munktell, Czech Republic). Chromatography was performed in pre-selected solvent systems: n-butanol‒acetic acid‒water (BAW) (4:1:2) ‒ I direction and 15% acetic acid ‒ II direction. Identification reactions were performed with aqueous and aqueous-alcoholic extracts of catalpa leaves. The qualitative composition of the phenolic compounds was investigated by paper chromatography. The content of the sum of phenolic compounds, hydroxycyclic acids and flavonoids was determined by the spectrophotometric method. Based on the held reactions and chromatographic analysis, by the characteristic fluorescence in the filtered UV light before and after treatment with chromogenic reagents and the values of Rf in the raw material the presence of the following groups of biologically active substances were determined: flavonoids, phenol glycosides, hydroxycoric acids. According to the chromatographic analysis, at least 9 substances of phenolic nature have been detected in the alcohol-aqueous extracts. For the first time, the presence of the following groups of biologically active substances: hydroxycoric acids, phenologic glycosides, flavonoids was determined in the leaves of catalpa. Quantitative content of natural compounds in the leaves of common catalpa: the sum of phenolic compounds (4,76 ± 0,04%), of hydroxycoric acids (3,42 ± 0,02%), of flavonoids (1,88 ± 0,02%). The results of the study will be used in the development of the draft quality control methods for medicinal plant raw materials.

scholarly journals Broccoli isothiocyanates content and in vitro availability according to variety and origin

2013 ◽  
Vol 32 (1) ◽  
pp. 251 ◽  
Author(s):  
M. Carmen Rodríguez-Hernández ◽  
Sonia Medina ◽  
Angel Gil-Izquierdo ◽  
Carmen Martínez-Ballesta ◽  
Diego A. Moreno
Keyword(s):  
Cancer Chemoprevention ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Active Compounds ◽  
In Vitro Availability ◽  
Different Sources

Broccoli is considered one of the healthiest vegetables own to its high content of beneficial biologically active compounds – the breakdown products of the glucosinolates, the isothiocyanates. The aim of this work was to characterize the production of isothiocyanates (sulforaphane and iberin and related metabolites) from different sources of glucosinolates by means of comparison between different broccoli cultivars and commercial samples, investigating their composition and in vitro bioavailability. Differences in the major intact glucosinolates were observed between the different experimental and commercial samples showing lower concentrations of glucosinolates the latest. The simulation of digestion reduced the concentration of parental phytochemicals (glucosinolates) producing the hydrolysis to biologically active isothiocyanates. The commercial samples showed lower levels of ITCs than experimental broccoli cultivars. Measurement of the glucosinolates ingested by UPLC-QqQ-MS/MS has allowed an exact quantifying of these compounds, particularly isothiocyanates, which will help to futures cancer chemoprevention studies.

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