Three-dimensional reconstruction using homocentric spherical spatiotemporal image analysis

1991 ◽  
Vol 2 (4) ◽  
pp. 365-372 ◽  
Author(s):  
Takayuki Yasuno ◽  
Teruo Hamano
Keyword(s):  
Image Analysis ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction

The interpretation of granitic textures from serial thin sectioning, image analysis and three-dimensional reconstruction

1995 ◽  
Vol 59 (395) ◽  
pp. 203-211 ◽  
Author(s):  
D. N. Bryon ◽  
M. P. Atherton ◽  
R. H. Hunter
Keyword(s):  
Image Analysis ◽  
Three Dimensional ◽  
Three Dimensional Reconstruction ◽  
Mineral Phase ◽  
Thin Sections ◽  
Thin Sectioning ◽  
Potential Problems ◽  
Dimensional Reconstruction ◽  
Textural Development

AbstractTextural development of the felsic phases in two granodioritic rocks from the zoned Linga superunit of the Peruvian Coastal Batholith has been characterized using serial thin sectioning, image analysis and three-dimensional reconstruction. The study has shown how each mineral phase contributed to the texture during the formation and development of a contiguous crystal framework and during subsequent restriction, isolation and occlusion of the melt-filled porosity. The work highlights the important factors and potential problems in the use of serial thin sections and imaging in the analysis of complex polyphase rock textures.

scholarly journals Three-dimensional reconstruction of thin filaments by electron cryo-microscopy and image analysis using back projection

1999 ◽  
Vol 39 (supplement) ◽  
pp. S187
Author(s):  
Akihiro Narita ◽  
Takashi Ishikawa ◽  
Kota Mayamagi ◽  
Takuo Yasunaga ◽  
Takeyuki Wakabayashi
Keyword(s):  
Image Analysis ◽  
Three Dimensional ◽  
Back Projection ◽  
Three Dimensional Reconstruction ◽  
Thin Filaments ◽  
Dimensional Reconstruction

scholarly journals From high symmetry to high resolution in biological electron microscopy: a commentary on Crowther (1971) ‘Procedures for three-dimensional reconstruction of spherical viruses by Fourier synthesis from electron micrographs’

2015 ◽  
Vol 370 (1666) ◽  
pp. 20140345 ◽  
Author(s):  
Peter B. Rosenthal
Keyword(s):  
Image Analysis ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Biological Macromolecules ◽  
High Symmetry ◽  
Three Dimensional Reconstruction ◽  
Fourier Synthesis ◽  
Negative Stain ◽  
Dimensional Reconstruction ◽  
Computational Procedures

Elucidation of the structure of biological macromolecules and larger assemblies has been essential to understanding the roles they play in living processes. Methods for three-dimensional structure determination of biological assemblies from images recorded in the electron microscope were therefore a key development. In his paper published in Philosophical Transactions B in 1971, Crowther described new computational procedures applied to the first three-dimensional reconstruction of an icosahedral virus from images of virus particles preserved in negative stain. The method for determining the relative orientation of randomly oriented particles and combining their images for reconstruction exploited the high symmetry of the virus particle. Computational methods for image analysis have since been extended to include biological assemblies without symmetry. Further experimental advances, combined with image analysis, have led to the method of cryomicroscopy, which is now used by structural biologists to study the structure and dynamics of biological machines and assemblies in atomic detail. This commentary was written to celebrate the 350th anniversary of the journal Philosophical Transactions of the Royal Society .

Methods for three-dimensional reconstruction of cellular components within a thick section

1986 ◽  
Vol 44 ◽  
pp. 18-21
Author(s):  
J. Frank ◽  
B. F. McEwen ◽  
M. Radermacher ◽  
C. L. Rieder
Keyword(s):  
Tilt Angle ◽  
Tomographic Reconstruction ◽  
3D Structure ◽  
Three Dimensional ◽  
Thick Section ◽  
Three Dimensional Reconstruction ◽  
Axis Ratio ◽  
Dimensional Reconstruction ◽  
Cellular Components

The tomographic reconstruction from multiple projections of cellular components, within a thick section, offers a way of visualizing and quantifying their three-dimensional (3D) structure. However, asymmetric objects require as many views from the widest tilt range as possible; otherwise the reconstruction may be uninterpretable. Even if not for geometric obstructions, the increasing pathway of electrons, as the tilt angle is increased, poses the ultimate upper limitation to the projection range. With the maximum tilt angle being fixed, the only way to improve the faithfulness of the reconstruction is by changing the mode of the tilting from single-axis to conical; a point within the object projected with a tilt angle of 60° and a full 360° azimuthal range is then reconstructed as a slightly elliptic (axis ratio 1.2 : 1) sphere.

Three-Dimensional Reconstruction of Native Androctonus Australis Hemocyanin

1990 ◽  
Vol 48 (1) ◽  
pp. 452-453
Author(s):  
Nicolas Boisset ◽  
Jean-Christophe Taveau ◽  
Jean Lamy ◽  
Terence Wagenknecht ◽  
Michael Radermacher ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Solid Body ◽  
Body Surface ◽  
Three Dimensional ◽  
Staining Technique ◽  
Three Dimensional Reconstruction ◽  
Dimensional Reconstruction ◽  
View Reconstruction ◽  
Surface Representations ◽  
Top View

Hemocyanin, the respiratory pigment of the scorpion Androctonus australis is composed of 24 kidney shaped subunits. A model of architecture supported by many indirect arguments has been deduced from electron microscopy (EM) and immuno-EM. To ascertain, the disposition of the subunits within the oligomer, the 24mer was submitted to three-dimensional reconstruction by the method of single-exposure random-conical tilt series.A sample of native hemocyanin, prepared with the double layer negative staining technique, was observed by transmisson electron microscopy under low-dose conditions. Six 3D-reconstructions were carried out indenpendently from top, side and 45°views. The results are composed of solid-body surface representations, and slices extracted from the reconstruction volume.The main two characters of the molecule previously reported by Van Heel and Frank, were constantly found in the solid-body surface representations. These features are the presence of two different faces called flip and flop and a rocking of the molecule around an axis passing through diagonnally opposed hexamers. Furthermore, in the solid-body surface of the top view reconstruction, the positions and orientations of the bridges connecting the half molecules were found in excellent agreement with those predicted by the model.

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