Radioactive tracer methods

Author(s):  
Douglas C. Eberle
Author(s):  
H. W. Hahn ◽  
K. Radermacher

In recent years the hydrodynamic theory of dynamically loaded engine bearings has been developed to such an extent that calculations of bearing behaviour under all kinds of loads can be made with good correlation to experimental results and field experience. In addition to these calculations, experiments have been performed to determine the influence of variables in practical bearings such as oil-inlet pressure or temperature, the shape of the oil inlet, and the quantity of the lubricant. At the engine-design stage, engine-bearing behaviour can be predicted by using calculations based on hydrodynamic theory. Some examples are given to show that the load-carrying capacity and consequently the reliability of engine bearings can be increased by using these calculation methods on a digital computer and with a variation of bearing characteristics, such as dimensions, clearance, oil pressure, temperature, and viscosity, or with a variation of load characteristics such as magnitude or direction of counterweight-mass forces, gas pressure, and firing order. Very good correlation is obtained between these predictions and real engine-bearing behaviour in fast-running diesel engines, as measured by radioactive tracer methods. Many German diesel-engine producers are using these calculations in an early stage of engine design.


1965 ◽  
Vol 49 (3) ◽  
pp. 471-478 ◽  
Author(s):  
Truls Brinck-Johnsen ◽  
Thomas F. Dougherly

ABSTRACT The effects of cortisol and ACTH in vivo on various mouse lymphatic tissues were studied, using the current techniques for isolation of adenine from nucleic acids and quantitative measurement, including chromatography, spectrophotometry and radioactive tracer methods. The incorporation of adenine-8-14C into both RNA and DNA of lymphatic tissues of intact mice was reduced following prolonged treatment with cortisol or ACTH. In the absence of the adrenals ACTH enhanced the incorporation of adenine-8-14C into the RNA of lymph nodes, spleen and thymus, although a clearcut effect on increased incorporation of the purine into DNA was limited to the spleen. It was concluded that ACTH has an extraadrenal effect on lymphatic tissues.


1960 ◽  
Vol 38 (1) ◽  
pp. 1235-1248 ◽  
Author(s):  
L. Gyenes ◽  
A. H. Sehon

Antigenically specific adsorbents were prepared by coupling soluble protein antigens to a polystyrene-supporting medium by azo bonds. These immunosorbents were shown to combine specifically with homologous precipitating rabbit antibodies. Elution of precipitating antibodies from the immunosorbents was achieved by dissociation with hydrochloric acid at pH 3. Under optimal conditions 35% of the antibodies removed from the antisera were recovered with a purity of over 80%. Electrophoretic analyses showed that precipitating rabbit antibodies were localized primarily in the region of gamma-globulins. The heterogeneity of the antigen–antibody systems studied was demonstrated by a combination of immunochemical, physicochemical, and radioactive tracer methods.


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